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The map label for this gene is mtnN

Identifier: 238920971

GI number: 238920971

Start: 2991066

End: 2991764

Strand: Direct

Name: mtnN

Synonym: NT01EI_3101

Alternate gene names: 238920971

Gene position: 2991066-2991764 (Clockwise)

Preceding gene: 238920968

Following gene: 238920972

Centisome position: 78.46

GC content: 64.52

Gene sequence:

>699_bases
ATGAAAGTAGGCATCATTGGCGCCATGGAGCAGGAAGTTACCCTGCTGCGCGACCGCATCGAAAATCGTCAGACCCTTAG
ACTGGCCAGCGGCGAAATCTATACTGGCCGCCTGCACGGCGTTGAGGTTGCGCTGCTGAAGTCCGGTATCGGCAAAGTCT
CTGCGGCGATGGGAACCACCCTGCTGCTGGATCACTGCCGCCCGGACGTGGTGATCAACACCGGCTCCGCCGGCGGCCTG
GCCAGCACCCTGCGGGTCGGCGACATCGTTATCTCCGACGAGGTGCGCTACCACGACGCCGACGTCACCGCCTTTGGCTA
TCAGCCGGGACAGATGGCCGGCTGTCCGGCGGCCTTTAGCGCCGATGCGGCGCTGATCGCCCTGGCCGAACGCTGCATCA
TCGCGCTGCAGCTGAACGCCGTGCGCGGCCTGATCTGCAGCGGTGACGCCTTCATCAACGGCGCCGAGCCGTTGGCCCGC
ATTCGCCGAACCTTCCCGCAGGTCGCCGCGGTAGAGATGGAAGCGGCGGCCATCGGCCACGTTTGCCACGCCTTCCAGAC
CCCGTTCGTGGTGGTGCGTGCCATCTCCGACGTCGCCGACCAGGAGTCCCATATCAGCTTTGATGAGTTCCTCAAGGTCG
CCGCCGAGCAGTCTACCCTGATGGTGGAAGCAATGCTGCGCGCAATGGCCTCCCAGTAA

Upstream 100 bases:

>100_bases
ATAATTTCCACCATTGCTGCCTTGCTCAGCAGTCCGGGAGCGAGCTATGGTAAACTGCGCATCCAGATTCCCGTTATTAC
TCCCAATTACAGGTATTGAT

Downstream 100 bases:

>100_bases
TCCATGGCCCTGCGACGCGCCAAACGCCGCCGCGGATCGGGCGGCATACTGTCGCTGGCTGCGCTGCTGCTGGGCCTCGG
TCTGAGCCTATCCCCGCTGC

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKVGIIGAMEQEVTLLRDRIENRQTLRLASGEIYTGRLHGVEVALLKSGIGKVSAAMGTTLLLDHCRPDVVINTGSAGGL
ASTLRVGDIVISDEVRYHDADVTAFGYQPGQMAGCPAAFSADAALIALAERCIIALQLNAVRGLICSGDAFINGAEPLAR
IRRTFPQVAAVEMEAAAIGHVCHAFQTPFVVVRAISDVADQESHISFDEFLKVAAEQSTLMVEAMLRAMASQ

Sequences:

>Translated_232_residues
MKVGIIGAMEQEVTLLRDRIENRQTLRLASGEIYTGRLHGVEVALLKSGIGKVSAAMGTTLLLDHCRPDVVINTGSAGGL
ASTLRVGDIVISDEVRYHDADVTAFGYQPGQMAGCPAAFSADAALIALAERCIIALQLNAVRGLICSGDAFINGAEPLAR
IRRTFPQVAAVEMEAAAIGHVCHAFQTPFVVVRAISDVADQESHISFDEFLKVAAEQSTLMVEAMLRAMASQ
>Mature_232_residues
MKVGIIGAMEQEVTLLRDRIENRQTLRLASGEIYTGRLHGVEVALLKSGIGKVSAAMGTTLLLDHCRPDVVINTGSAGGL
ASTLRVGDIVISDEVRYHDADVTAFGYQPGQMAGCPAAFSADAALIALAERCIIALQLNAVRGLICSGDAFINGAEPLAR
IRRTFPQVAAVEMEAAAIGHVCHAFQTPFVVVRAISDVADQESHISFDEFLKVAAEQSTLMVEAMLRAMASQ

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=74.3478260869565, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_EDWI9 (C5BAP4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934486.1
- GeneID:   7961870
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3101
- OMA:   AMEQEVT
- ProtClustDB:   PRK05584
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 24636; Mature: 24636

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 152-152 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIIGAMEQEVTLLRDRIENRQTLRLASGEIYTGRLHGVEVALLKSGIGKVSAAMGTT
CCEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEHEEHHHCCHHHHHHHHHH
LLLDHCRPDVVINTGSAGGLASTLRVGDIVISDEVRYHDADVTAFGYQPGQMAGCPAAFS
HHHHCCCCCEEEECCCCCCHHHHHEECEEEEECCCEEECCCEEEECCCCCCCCCCCCCCC
ADAALIALAERCIIALQLNAVRGLICSGDAFINGAEPLARIRRTFPQVAAVEMEAAAIGH
CCHHHHHHHHHHHEEEEEHHHHEEEECCCCEECCCHHHHHHHHHCHHHHHHHHHHHHHHH
VCHAFQTPFVVVRAISDVADQESHISFDEFLKVAAEQSTLMVEAMLRAMASQ
HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGIIGAMEQEVTLLRDRIENRQTLRLASGEIYTGRLHGVEVALLKSGIGKVSAAMGTT
CCEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEHEEHHHCCHHHHHHHHHH
LLLDHCRPDVVINTGSAGGLASTLRVGDIVISDEVRYHDADVTAFGYQPGQMAGCPAAFS
HHHHCCCCCEEEECCCCCCHHHHHEECEEEEECCCEEECCCEEEECCCCCCCCCCCCCCC
ADAALIALAERCIIALQLNAVRGLICSGDAFINGAEPLARIRRTFPQVAAVEMEAAAIGH
CCHHHHHHHHHHHEEEEEHHHHEEEECCCCEECCCHHHHHHHHHCHHHHHHHHHHHHHHH
VCHAFQTPFVVVRAISDVADQESHISFDEFLKVAAEQSTLMVEAMLRAMASQ
HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA