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The map label for this gene is mrdB [H]

Identifier: 238920817

GI number: 238920817

Start: 2831498

End: 2832610

Strand: Direct

Name: mrdB [H]

Synonym: NT01EI_2943

Alternate gene names: 238920817

Gene position: 2831498-2832610 (Clockwise)

Preceding gene: 238920816

Following gene: 238920818

Centisome position: 74.27

GC content: 58.94

Gene sequence:

>1113_bases
ATGACTGAGAATCAACAAAAGCGCTCAATCTGGGCCAAGATGCATATCGACCTGCCCTTCCTGCTCTGCATTCTGGCGGT
GCTGGTCTACAGCGCCATCGTCATGTGGAGCGCCAGCGGCCAGGATCTCGGCATGATGGAGCGCAAGATCGGCCAAATCG
TCATGGGCCTGCTGGTCATGATCGTCATGGCGCAGATACCGCCGCGCGTTTACGAACACTGGGCGCCCTATCTGTATATC
CTCTGCGTCATCCTGCTGATCCTGGTGGATGCCTTCGGCCAGATCAGTAAAGGCGCCCAGCGCTGGCTTGACCTTGGTTT
CGTGCGCTTTCAGCCGTCGGAGATCGCCAAAATCGCCGTACCGCTGATGGTGGCACGCTTTATCAACCGCGACGTCTGCC
CACCGTCGCTCAAGCACACCGGCATCGCGCTGATCCTGATCTTCCTGCCGACCCTGCTGGTCGCAGCCCAACCGGATCTG
GGCACCTCGATCCTGGTCGCCGCCTCCGGGCTGTTCATTCTGTTCCTGGCGGGCATGAGCTGGCGCCTGATCGGACTGGC
CGTGCTGTTGGTGGCTGCCTTTATTCCGGTGCTATGGTTCTTCCTGATGCACGACTACCAGCGCGACCGGGTGATGATGC
TGCTCGATCCGGAAAGCGATCCGCTGGGCGCCGGCTACCACATCATCCAATCAAAAATCGCCATCGGCTCCGGCGGCCTG
AGCGGCAAGGGGTGGCTACACGGCACCCAGTCACAGCTGGAGTTCCTGCCGGAGCGCCACACCGACTTTATCTTCGCCGT
GCTGGCCGAAGAGCTGGGGCTGTTCGGCGTACTGCTGCTGCTGGCGCTATACCTGCTGATCATCATGCGCGGACTGTATA
TCGCCGCCCGCGCCCAGACCACCTTCGGCCGGGTGATGGTCGGCGGTTTAATGTTGATTCTATTCGTTTATGTTTTCGTT
AATATCGGTATGGTGAGTGGCATTTTACCGGTGGTTGGCGTACCTTTGCCTCTGGTCAGCTACGGTGGCTCGGCGCTGAT
CGTACTGATGGCCGGGTTTGGCATCGTGATGTCGATCCATACCCATCGCAAAATGTTATCCAAGAATCTATAG

Upstream 100 bases:

>100_bases
CCTGGATCACATCATGCTGGGCGACAACAATACCCAGCTTCCCAATGCAGCCCCCACGCCCCCCGGCGTGGAGGGCGACT
GACAGGCATACGGATTCACC

Downstream 100 bases:

>100_bases
AGGTCGCGATGAGAATGGCGCACAAATCCTGGCTCAGCCTGAGCCTGGCCTGTCTGATCCTGGCAGGCTGTTCCACCAAC
GACAACGGTGGGACCCAGGC

Product: cell wall shape-determining protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTENQQKRSIWAKMHIDLPFLLCILAVLVYSAIVMWSASGQDLGMMERKIGQIVMGLLVMIVMAQIPPRVYEHWAPYLYI
LCVILLILVDAFGQISKGAQRWLDLGFVRFQPSEIAKIAVPLMVARFINRDVCPPSLKHTGIALILIFLPTLLVAAQPDL
GTSILVAASGLFILFLAGMSWRLIGLAVLLVAAFIPVLWFFLMHDYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLFGVLLLLALYLLIIMRGLYIAARAQTTFGRVMVGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKNL

Sequences:

>Translated_370_residues
MTENQQKRSIWAKMHIDLPFLLCILAVLVYSAIVMWSASGQDLGMMERKIGQIVMGLLVMIVMAQIPPRVYEHWAPYLYI
LCVILLILVDAFGQISKGAQRWLDLGFVRFQPSEIAKIAVPLMVARFINRDVCPPSLKHTGIALILIFLPTLLVAAQPDL
GTSILVAASGLFILFLAGMSWRLIGLAVLLVAAFIPVLWFFLMHDYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLFGVLLLLALYLLIIMRGLYIAARAQTTFGRVMVGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKNL
>Mature_369_residues
TENQQKRSIWAKMHIDLPFLLCILAVLVYSAIVMWSASGQDLGMMERKIGQIVMGLLVMIVMAQIPPRVYEHWAPYLYIL
CVILLILVDAFGQISKGAQRWLDLGFVRFQPSEIAKIAVPLMVARFINRDVCPPSLKHTGIALILIFLPTLLVAAQPDLG
TSILVAASGLFILFLAGMSWRLIGLAVLLVAAFIPVLWFFLMHDYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGLS
GKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLFGVLLLLALYLLIIMRGLYIAARAQTTFGRVMVGGLMLILFVYVFVN
IGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKNL

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=370, Percent_Identity=86.4864864864865, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786277, Length=319, Percent_Identity=32.2884012539185, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 40786; Mature: 40655

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENQQKRSIWAKMHIDLPFLLCILAVLVYSAIVMWSASGQDLGMMERKIGQIVMGLLVM
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IVMAQIPPRVYEHWAPYLYILCVILLILVDAFGQISKGAQRWLDLGFVRFQPSEIAKIAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHH
PLMVARFINRDVCPPSLKHTGIALILIFLPTLLVAAQPDLGTSILVAASGLFILFLAGMS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGLAVLLVAAFIPVLWFFLMHDYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLFGVLLLLALYLLIIMRGLYIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMVGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKNL
HHHHHHHCCC
>Mature Secondary Structure 
TENQQKRSIWAKMHIDLPFLLCILAVLVYSAIVMWSASGQDLGMMERKIGQIVMGLLVM
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IVMAQIPPRVYEHWAPYLYILCVILLILVDAFGQISKGAQRWLDLGFVRFQPSEIAKIAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHH
PLMVARFINRDVCPPSLKHTGIALILIFLPTLLVAAQPDLGTSILVAASGLFILFLAGMS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGLAVLLVAAFIPVLWFFLMHDYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLFGVLLLLALYLLIIMRGLYIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMVGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKNL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]