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The map label for this gene is rfaE [H]

Identifier: 238896551

GI number: 238896551

Start: 4529045

End: 4530478

Strand: Reverse

Name: rfaE [H]

Synonym: KP1_4741

Alternate gene names: 238896551

Gene position: 4530478-4529045 (Counterclockwise)

Preceding gene: 238896552

Following gene: 238896550

Centisome position: 86.32

GC content: 61.65

Gene sequence:

>1434_bases
ATGAAAGTAACGCTGCCGGAGTTTGAACGTGCAGGAGTGTTGGTGGTGGGTGATGTGATGCTGGACCGCTACTGGTACGG
CCCCACCAGTCGTATTTCCCCGGAAGCCCCGGTGCCGGTGGTGAAGGTGGAAAATATCGAAGAACGTCCTGGCGGCGCGG
CAAACGTAGCGATGAACATTGCCTCGCTGGGGGCAACCTCGCGCCTGGTGGGATTGACCGGGATTGATGACGCCGCCCGC
GCGCTGAGCCAGGCGCTGGCCAACGTCAATGTGAAGTGCGACTTCGTCTCCGTCCCGACTCACCCGACTATCACCAAGCT
GCGGGTGCTGTCGCGCAATCAACAGCTGATCCGCCTCGACTTTGAAGAGGGCTTCTCCGGCGTCGATCCGCAGCCGATGC
ACGAGCGCATTCAGCAGGCGCTGGGCTCTATTGGCGCGCTGGTCCTTTCGGACTATGCCAAAGGGGCGCTGACCAGCGTC
CAGACCATGATCCGACTCGCACGTGAAGCGGGCGTGCCGGTGCTGATCGATCCGAAAGGCACCGATTTCGAACGCTATCG
CGGCGCGACGTTGCTCACCCCGAACCTCTCTGAGTTTGAAGCGGTGGTGGGCAAGTGCCAGGATGAAGCGCAGATCGTTG
AGCGCGGCATGAAGCTCATCGCCGAATTCGAACTGTCGGCGCTGCTGGTGACGCGCTCTGAGCAGGGGATGACGCTGCTG
CAGCCGGGACGTCCGCCGCTGCATATGCCGACCCAGGCCCAGGAAGTGTACGACGTGACCGGCGCCGGCGACACGGTGAT
TGGCGTGCTGGCGGCGACCCTGGCGTCCGGCAACACCCTGGAAGAGGCCTGCTATTTCGCTAACGCCGCGGCGGGCGTGG
TGGTCGGTAAGCTGGGTACATCCACCGTTTCCCCTGTCGAGTTGGAAAACGCGGTCCGCGGCCGGGCTGAGACCGGCTTT
GGCGTGATGAGCGAAGAGGAACTCAAACAGGCGGTTGCGGCGGCGCGCAAGCGCGGCGAGAAAGTGGTGATGACCAACGG
CGTCTTCGACATTCTGCATGCCGGTCATGTCTCCTATCTGGCGAATGCGCGCAAGCTGGGCGACCGTCTGATCGTGGCGG
TCAACAGCGATGCCTCCACCAAACGTCTGAAAGGCGAAACCCGCCCGGTGAACCCGCTGGAACAGCGGATGATCGTGCTG
GGGGCGCTGGAGGCCGTCGACTGGGTGGTCTCCTTCGAAGAAGATACTCCGCAGCGCCTGATCGCTGGCATCCTGCCGGA
TCTGCTGGTCAAGGGCGGCGACTACAAACCGGAGCAAATCGCCGGCAGCGAAGAGGTCTGGGCGAACGGTGGCGAAGTGC
TGGTGCTCAACTTTGAAGATGGCTGCTCAACTACCAATATTATTAAGAAAATACAAAAAGATAGCGACAAATAA

Upstream 100 bases:

>100_bases
GAGCAGGTCAGCGCCAGCTGGCAGAAATGGCTGATGGCTTAACTATAAAATCGGGTGTGCTATTATCGCGCGCAAAGTTT
GCGTCTCGCAGGAGAGAGTC

Downstream 100 bases:

>100_bases
GCTTTTCGCTATTCACCGGTTTGTACGGTGAAGCATCACGGCCTGCGATAGACGTATCGCGGGCCGTTTTTATTTAATCA
TGTGGAAAATGATTTAGCGC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKVTLPEFERAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVENIEERPGGAANVAMNIASLGATSRLVGLTGIDDAAR
ALSQALANVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSGVDPQPMHERIQQALGSIGALVLSDYAKGALTSV
QTMIRLAREAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCQDEAQIVERGMKLIAEFELSALLVTRSEQGMTLL
QPGRPPLHMPTQAQEVYDVTGAGDTVIGVLAATLASGNTLEEACYFANAAAGVVVGKLGTSTVSPVELENAVRGRAETGF
GVMSEEELKQAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVLVLNFEDGCSTTNIIKKIQKDSDK

Sequences:

>Translated_477_residues
MKVTLPEFERAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVENIEERPGGAANVAMNIASLGATSRLVGLTGIDDAAR
ALSQALANVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSGVDPQPMHERIQQALGSIGALVLSDYAKGALTSV
QTMIRLAREAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCQDEAQIVERGMKLIAEFELSALLVTRSEQGMTLL
QPGRPPLHMPTQAQEVYDVTGAGDTVIGVLAATLASGNTLEEACYFANAAAGVVVGKLGTSTVSPVELENAVRGRAETGF
GVMSEEELKQAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVLVLNFEDGCSTTNIIKKIQKDSDK
>Mature_477_residues
MKVTLPEFERAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVENIEERPGGAANVAMNIASLGATSRLVGLTGIDDAAR
ALSQALANVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSGVDPQPMHERIQQALGSIGALVLSDYAKGALTSV
QTMIRLAREAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCQDEAQIVERGMKLIAEFELSALLVTRSEQGMTLL
QPGRPPLHMPTQAQEVYDVTGAGDTVIGVLAATLASGNTLEEACYFANAAAGVVVGKLGTSTVSPVELENAVRGRAETGF
GVMSEEELKQAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVLVLNFEDGCSTTNIIKKIQKDSDK

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=474, Percent_Identity=92.1940928270042, Blast_Score=901, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 51152; Mature: 51152

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLPEFERAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVENIEERPGGAANVAMNI
CCCCCCCHHCCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
ASLGATSRLVGLTGIDDAARALSQALANVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCHHEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFSGVDPQPMHERIQQALGSIGALVLSDYAKGALTSVQTMIRLAREAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCQDEAQIVERGMKLIAEFELSALLVTRSEQGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEE
QPGRPPLHMPTQAQEVYDVTGAGDTVIGVLAATLASGNTLEEACYFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPVELENAVRGRAETGFGVMSEEELKQAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVLVLNFEDGCSTTNIIKKIQKDSDK
HHHHHHHHHHCCCCCCHHHCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVTLPEFERAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVENIEERPGGAANVAMNI
CCCCCCCHHCCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
ASLGATSRLVGLTGIDDAARALSQALANVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCHHEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFSGVDPQPMHERIQQALGSIGALVLSDYAKGALTSVQTMIRLAREAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCQDEAQIVERGMKLIAEFELSALLVTRSEQGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEE
QPGRPPLHMPTQAQEVYDVTGAGDTVIGVLAATLASGNTLEEACYFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPVELENAVRGRAETGFGVMSEEELKQAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGETRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEQIAGSEEVWANGGEVLVLNFEDGCSTTNIIKKIQKDSDK
HHHHHHHHHHCCCCCCHHHCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA