Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 238896538

GI number: 238896538

Start: 4517360

End: 4519255

Strand: Reverse

Name: parE [H]

Synonym: KP1_4725

Alternate gene names: 238896538

Gene position: 4519255-4517360 (Counterclockwise)

Preceding gene: 238896539

Following gene: 238896532

Centisome position: 86.11

GC content: 59.02

Gene sequence:

>1896_bases
ATGACGCAATCTTCATATAATGCTGATGCCATTGAGGTACTCACCGGGCTAGAGCCGGTGCGCCGCCGTCCGGGCATGTA
TACCGACACCACTCGTCCTAACCATCTTGGTCAGGAAGTTATTGATAACAGTGTGGATGAAGCGCTGGCAGGCCACGCCA
AACGCGTGGAAGTTATCCTTCATGCCGACCAGTCCCTGGAGGTCATTGACGATGGTCGTGGGATGCCGGTCGACATCCAC
CCGGAAGAGGGCGTGCCAGCGGTTGAGCTGATCCTCTGCCGGCTGCATGCGGGCGGTAAGTTTTCCAATAAAAACTATCA
GTTTTCCGGCGGCCTGCACGGGGTGGGGATCTCGGTAGTCAACGCCCTGTCAAAACGCGTGGAGGTCAACGTGCGTCGCG
ACGGTCAGGTCTACAGTATCGCCTTTGAAAATGGTGAAAAAGTGGAAGACCTGCACGTCACCGGCACCTGCGGCAAACGC
AATACCGGGACCAGCGTCCATTTCTGGCCGGACGAAAGCTTCTTCGACAGCCCGCGCTTTTCCGTTTCACGTCTGACTCA
CCTGTTAAAAGCGAAAGCGGTGCTGTGCCCGGGCGTGGAAATTGTTTTCCGCGACCAGGTGAACAACAGCGAGCAGAGCT
GGTGCTATGCCGATGGCCTGAACGATTACCTGAGCGAAGCGGTGAACGGCCTGCCGCTGCTGCCGGAAAAACCATTCGTC
GGCGCCTTCTCCGGCGAGACGGAGGCGGTGGACTGGGCGCTGCTGTGGCTGCCGGAAGGCGGCGAACTGCTGACCGAGAG
CTACGTGAACCTGATCCCGACTATGCAGGGCGGGACGCACGTTAACGGTCTGCGTCAGGGGCTGCTCGACGCGATGCGTG
AGTTCTGCGAGTACCGTAATATCCTGCCGCGCGGCGTGAAGCTGTCAGCGGAAGATATCTGGGATCGCTGCGCGTACGTG
CTGTCGGTGAAGATGCAGGATCCGCAGTTCGCCGGGCAAACCAAAGAGCGCCTGTCGTCGCGCCAGTGCGCGGCGTTCGT
CTCCGGCGTGGTGAAGGACGCCTTCAGCCTGTGGCTGAATCAGAACGTGCAGGCCGCGGAGCTGCTGGCGGAGATGGCCA
TCTCCAGCGCCCAGCGCCGTCTGCGCGCCGCCAAAAAAGTGGTGCGCAAAAAGCTGACCAGCGGTCCGGCGCTGCCGGGT
AAGCTGGCGGACTGTACCGCCCAGGATCTGAACCGTACCGAGCTGTTCCTCGTCGAAGGGGATTCGGCGGGCGGCTCAGC
CAAGCAGGCGCGCGATCGCGAATATCAGGCGATCATGCCGCTGAAGGGCAAGATCCTTAATACCTGGGAAGTCTCTTCCG
ATGAAGTGTTAGCTTCGCAAGAAGTGCACGACATTTCGGTGGCGATTGGTATTGACCCGGATAGCGATGATTTAAGCCAG
CTGCGCTACGGTAAAATTTGTATTCTGGCGGATGCGGACTCCGATGGTCTGCACATTGCCACCCTGCTGTGCGCGCTGTT
CGTAAGACATTTCCGTACCCTCGTGAAAGAGGGGCACGTCTACGTGGCGCTGCCGCCGCTGTACCGTATCGACCTCGGCA
AAGAAGTTTACTACGCGCTGACGGAAGAAGAAAAAACCGGCGTGCTGGAACAGCTAAAGCGCAAGAAGGGCAAGCCAAAC
GTCCAGCGCTTTAAAGGGCTCGGTGAGATGAACCCGATGCAGCTGCGCGAAACCACCCTTGACCCGAATACCCGCCGCCT
GGTGCAGCTGGTGATAAGCGATGAGGACGAGCAACAGACCACGGCGATAATGGATATGCTGCTGGCCAAAAAGCGTTCGG
AAGATCGTCGCAACTGGCTGCAGGAGAAGGGCGATATGGCGGATCTGGAAGTGTAA

Upstream 100 bases:

>100_bases
CGGTAATCACGCATTTGTCGGCTTTGACGATCATTTCACTCAGATTATCGAATTTCTCGGCCTGCGCTAAACGGCCCTGA
ACGACCTACGAATACACACC

Downstream 100 bases:

>100_bases
GACCGCACGCTGCGCCAGACGTAAAAGGGAGCCTCAGGCTCCCTTTTCTTATTCCGTTTCAGCGTCGGCGGGCTTAGACG
CCGGCCTGCAGGATACGGAT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MTQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVILHADQSLEVIDDGRGMPVDIH
PEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYSIAFENGEKVEDLHVTGTCGKR
NTGTSVHFWPDESFFDSPRFSVSRLTHLLKAKAVLCPGVEIVFRDQVNNSEQSWCYADGLNDYLSEAVNGLPLLPEKPFV
GAFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYV
LSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPG
KLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQ
LRYGKICILADADSDGLHIATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYALTEEEKTGVLEQLKRKKGKPN
VQRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQTTAIMDMLLAKKRSEDRRNWLQEKGDMADLEV

Sequences:

>Translated_631_residues
MTQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVILHADQSLEVIDDGRGMPVDIH
PEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYSIAFENGEKVEDLHVTGTCGKR
NTGTSVHFWPDESFFDSPRFSVSRLTHLLKAKAVLCPGVEIVFRDQVNNSEQSWCYADGLNDYLSEAVNGLPLLPEKPFV
GAFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYV
LSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPG
KLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQ
LRYGKICILADADSDGLHIATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYALTEEEKTGVLEQLKRKKGKPN
VQRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQTTAIMDMLLAKKRSEDRRNWLQEKGDMADLEV
>Mature_630_residues
TQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYSIAFENGEKVEDLHVTGTCGKRN
TGTSVHFWPDESFFDSPRFSVSRLTHLLKAKAVLCPGVEIVFRDQVNNSEQSWCYADGLNDYLSEAVNGLPLLPEKPFVG
AFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYALTEEEKTGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQTTAIMDMLLAKKRSEDRRNWLQEKGDMADLEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=575, Percent_Identity=24.695652173913, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=589, Percent_Identity=23.7691001697793, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1789408, Length=631, Percent_Identity=94.2947702060222, Blast_Score=1244, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=41.1870503597122, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI212645845, Length=606, Percent_Identity=24.2574257425743, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535065, Length=632, Percent_Identity=23.8924050632911, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=252, Percent_Identity=25.7936507936508, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=655, Percent_Identity=23.8167938931298, Blast_Score=136, Evalue=9e-33,
Organism=Drosophila melanogaster, GI17136538, Length=581, Percent_Identity=22.5473321858864, Blast_Score=114, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70076; Mature: 69945

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVIL
CCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVV
ECCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHH
NALSKRVEVNVRRDGQVYSIAFENGEKVEDLHVTGTCGKRNTGTSVHFWPDESFFDSPRF
HHHHHHEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCH
SVSRLTHLLKAKAVLCPGVEIVFRDQVNNSEQSWCYADGLNDYLSEAVNGLPLLPEKPFV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCC
GAFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRN
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLN
CCCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVQAAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEEC
DSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQ
CCCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHH
LRYGKICILADADSDGLHIATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYAL
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCEEEEEE
TEEEKTGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQT
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCHHHHEEEEECCCCHHHH
TAIMDMLLAKKRSEDRRNWLQEKGDMADLEV
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVIL
CCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVV
ECCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHH
NALSKRVEVNVRRDGQVYSIAFENGEKVEDLHVTGTCGKRNTGTSVHFWPDESFFDSPRF
HHHHHHEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCH
SVSRLTHLLKAKAVLCPGVEIVFRDQVNNSEQSWCYADGLNDYLSEAVNGLPLLPEKPFV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCC
GAFSGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRN
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLN
CCCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVQAAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEEC
DSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQ
CCCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHH
LRYGKICILADADSDGLHIATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYAL
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCEEEEEE
TEEEKTGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQT
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCHHHHEEEEECCCCHHHH
TAIMDMLLAKKRSEDRRNWLQEKGDMADLEV
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]