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The map label for this gene is gss [H]

Identifier: 238896510

GI number: 238896510

Start: 4489530

End: 4491395

Strand: Reverse

Name: gss [H]

Synonym: KP1_4691

Alternate gene names: 238896510

Gene position: 4491395-4489530 (Counterclockwise)

Preceding gene: 238896513

Following gene: 238896509

Centisome position: 85.57

GC content: 57.13

Gene sequence:

>1866_bases
ATGAGCAAAGGATCGACAAGCAGTGACGCCCCGTTTGGGACATTGTTAGGCTATGCACCCGGTGGCGTAGCGATTTATTC
ATCGAATTACAGTAGCTTAAATCCGCAGGACTACCCGGATGACGCGACGTTTCGCAGCTACATCGGCAATGAGTATATGG
GGCATAAATGGCAGTGCGTGGAGTTTGCGCGCCGCTTTCTGTTCCTGACCTATGGCTTTGTCTTCACCGATGTGGGGATG
GCTTATGAAATCTTCTCCCTGCGTTTCCTGCGCGAAGTGGTCAATGACAATATTTTGCCGCTGCAGGCCTTCGCCAACGG
CTCCCGCCGTCCGCCTATCGCTGGTTCGCTGCTGATCTGGCAGAAGGGGGGCGAGTTTAAGCACACCGGCCATGTGGCGG
TGATCACCCAGCTTGTCGGCAATAAGGTGCGTATCGCCGAGCAGAACGTGATTCACTCGCCGCTGCCGCAGGGCCAGCAG
TGGACCCGCGAGCTGACCCTCGAGGTCAACGACGGCCGGTACACGATCAAAGATACCTTTGCCGATACCGAAATTTTAGG
CTGGATGATCCAGACCGCTGATACCGAACACAGCCTGCCGCAGCCGGTGCTGCCGGGCGAGGCGATGGCCATCAAAGGCG
CGCGGCTGCCAAACAACGGCCAGTTTCGCGGTAAATGGCTGAACGAGAAAGACCCGCTGCAGAAAGCCTACGTGGCAGCC
AACGGTCATTTCATCAACCAGGATCCCTATCAGTATTTCACCATCAGCGAAAGTGCCGAACAGGAGCTGATCAAAGCCAC
CAACGAGCTGCACCTGATGTATCTGCACGCCACCGATAAGGTGATGAAGGATGACAACCTGCTGGCGCTGTTCGATATTC
CGAAAATTCTCTGGCCTCGTCTGCGCCTCTCGTGGCAGCGCCGCCGTCATCATATGATCACCGGGCGCATGGATTTTTGC
ATGGACGAGCGCGGGCTGAAGGTGTACGAGTACAATGCCGATTCCGCTTCCTGCCATACCGAAGGCGGGCTGATCCTCGA
ACAGTGGCTGAAGCAGGGCTACTACGGCACCGGCCACAACCCGGCGGAAGGTCTGCTCGATGAGCTGGCGGGGGCGTGGA
AACACAGCCGGGCGCGGCCCTTCGTCCACATCATGCAGGACAAGGATCTGGAAGAGAACTACCACGCGCAGTTTATTCAG
CGTTCGCTGACCCAGGCCGGTTTTGAGAGCAAAATTCTCTTTGGCCTTGATGAGCTGCGCTGGGACGCTGCCGGTCAGCT
GATCGACGCCGACGGGCGGCTGGTCAACTGCGTCTGGAAAACCTGGGCATGGGAAACCGCCATTGAGCAGGTGCGCGAAG
TCAGCGCCGAGGAGTACGCGGCGGTACCGATTCGTACCGGACATCCGCAGAATGAGGTGCGGCTGATTGACGTCCTGCTG
CGTCCTGAAGTATTGGTATTCGAACCCCTGTGGACGGTGATCCCCGGCAACAAAGCCATTCTGCCGGTGCTGTGGTCGCT
GTTCCCGCATCATCGCTATCTGCTGGATACCGATTTTGAGGTTAACGACGAGCTGGCGAAAACCGGCTATGCGGTGAAGC
CTATTTCCGGGCGCTGCGGCAACAATATCGACCTGATCGGCCCTCAGGATGAACTTCTGGATAAGACCAGCGGCCAGTTT
GTCGATCGCAAGAATATCTACCAGCAGCTGTGGTGCCTGCCGAAAGTGGACGGTAAGTACATTCAGGTGTGCACATTTAC
CGTCGGCGGTAACTACGGCGGTACCTGCCTGCGCGGCGATTCGTCGCTGGTGGTGAAAAAAGAGAGCGATATCGAGCCGT
TAATCGTTTTGAAAGACAAAGCATGA

Upstream 100 bases:

>100_bases
CATCGTTACACCTCTGTCTGGTAAGTGCCTGCGGCAGGATAAGAAGAGGGAGTTTTGTTTCGCCAACTGCCCGGGCTGCA
TTAATTTGAGGTCAGGCTAA

Downstream 100 bases:

>100_bases
CGTACGAAACCCGGGTAGCGGCATACCCCTGTCCGGGTGACAACGTACTGAAAAGGATAGCCCCGGCATGGTAAGCGCCG
CCGGGGAGCACCTGCAACAA

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MSKGSTSSDAPFGTLLGYAPGGVAIYSSNYSSLNPQDYPDDATFRSYIGNEYMGHKWQCVEFARRFLFLTYGFVFTDVGM
AYEIFSLRFLREVVNDNILPLQAFANGSRRPPIAGSLLIWQKGGEFKHTGHVAVITQLVGNKVRIAEQNVIHSPLPQGQQ
WTRELTLEVNDGRYTIKDTFADTEILGWMIQTADTEHSLPQPVLPGEAMAIKGARLPNNGQFRGKWLNEKDPLQKAYVAA
NGHFINQDPYQYFTISESAEQELIKATNELHLMYLHATDKVMKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEGGLILEQWLKQGYYGTGHNPAEGLLDELAGAWKHSRARPFVHIMQDKDLEENYHAQFIQ
RSLTQAGFESKILFGLDELRWDAAGQLIDADGRLVNCVWKTWAWETAIEQVREVSAEEYAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPVLWSLFPHHRYLLDTDFEVNDELAKTGYAVKPISGRCGNNIDLIGPQDELLDKTSGQF
VDRKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDSSLVVKKESDIEPLIVLKDKA

Sequences:

>Translated_621_residues
MSKGSTSSDAPFGTLLGYAPGGVAIYSSNYSSLNPQDYPDDATFRSYIGNEYMGHKWQCVEFARRFLFLTYGFVFTDVGM
AYEIFSLRFLREVVNDNILPLQAFANGSRRPPIAGSLLIWQKGGEFKHTGHVAVITQLVGNKVRIAEQNVIHSPLPQGQQ
WTRELTLEVNDGRYTIKDTFADTEILGWMIQTADTEHSLPQPVLPGEAMAIKGARLPNNGQFRGKWLNEKDPLQKAYVAA
NGHFINQDPYQYFTISESAEQELIKATNELHLMYLHATDKVMKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEGGLILEQWLKQGYYGTGHNPAEGLLDELAGAWKHSRARPFVHIMQDKDLEENYHAQFIQ
RSLTQAGFESKILFGLDELRWDAAGQLIDADGRLVNCVWKTWAWETAIEQVREVSAEEYAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPVLWSLFPHHRYLLDTDFEVNDELAKTGYAVKPISGRCGNNIDLIGPQDELLDKTSGQF
VDRKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDSSLVVKKESDIEPLIVLKDKA
>Mature_620_residues
SKGSTSSDAPFGTLLGYAPGGVAIYSSNYSSLNPQDYPDDATFRSYIGNEYMGHKWQCVEFARRFLFLTYGFVFTDVGMA
YEIFSLRFLREVVNDNILPLQAFANGSRRPPIAGSLLIWQKGGEFKHTGHVAVITQLVGNKVRIAEQNVIHSPLPQGQQW
TRELTLEVNDGRYTIKDTFADTEILGWMIQTADTEHSLPQPVLPGEAMAIKGARLPNNGQFRGKWLNEKDPLQKAYVAAN
GHFINQDPYQYFTISESAEQELIKATNELHLMYLHATDKVMKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEGGLILEQWLKQGYYGTGHNPAEGLLDELAGAWKHSRARPFVHIMQDKDLEENYHAQFIQR
SLTQAGFESKILFGLDELRWDAAGQLIDADGRLVNCVWKTWAWETAIEQVREVSAEEYAAVPIRTGHPQNEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPVLWSLFPHHRYLLDTDFEVNDELAKTGYAVKPISGRCGNNIDLIGPQDELLDKTSGQFV
DRKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDSSLVVKKESDIEPLIVLKDKA

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=618, Percent_Identity=84.4660194174757, Blast_Score=1108, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=391, Percent_Identity=26.0869565217391, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790629, Length=370, Percent_Identity=27.5675675675676, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 70433; Mature: 70302

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGSTSSDAPFGTLLGYAPGGVAIYSSNYSSLNPQDYPDDATFRSYIGNEYMGHKWQCV
CCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
EFARRFLFLTYGFVFTDVGMAYEIFSLRFLREVVNDNILPLQAFANGSRRPPIAGSLLIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCEEEEE
QKGGEFKHTGHVAVITQLVGNKVRIAEQNVIHSPLPQGQQWTRELTLEVNDGRYTIKDTF
ECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECC
ADTEILGWMIQTADTEHSLPQPVLPGEAMAIKGARLPNNGQFRGKWLNEKDPLQKAYVAA
CCHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEE
NGHFINQDPYQYFTISESAEQELIKATNELHLMYLHATDKVMKDDNLLALFDIPKILWPR
CCEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEEECCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEGGLILEQWLKQGYYGTGHN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PAEGLLDELAGAWKHSRARPFVHIMQDKDLEENYHAQFIQRSLTQAGFESKILFGLDELR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHH
WDAAGQLIDADGRLVNCVWKTWAWETAIEQVREVSAEEYAAVPIRTGHPQNEVRLIDVLL
HHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPVLWSLFPHHRYLLDTDFEVNDELAKTGYAVKPISGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEEECCCCCCC
NNIDLIGPQDELLDKTSGQFVDRKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCEEECCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
SSLVVKKESDIEPLIVLKDKA
CCEEEEECCCCCCEEEEECCC
>Mature Secondary Structure 
SKGSTSSDAPFGTLLGYAPGGVAIYSSNYSSLNPQDYPDDATFRSYIGNEYMGHKWQCV
CCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
EFARRFLFLTYGFVFTDVGMAYEIFSLRFLREVVNDNILPLQAFANGSRRPPIAGSLLIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCEEEEE
QKGGEFKHTGHVAVITQLVGNKVRIAEQNVIHSPLPQGQQWTRELTLEVNDGRYTIKDTF
ECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECC
ADTEILGWMIQTADTEHSLPQPVLPGEAMAIKGARLPNNGQFRGKWLNEKDPLQKAYVAA
CCHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEE
NGHFINQDPYQYFTISESAEQELIKATNELHLMYLHATDKVMKDDNLLALFDIPKILWPR
CCEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEEECCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEGGLILEQWLKQGYYGTGHN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PAEGLLDELAGAWKHSRARPFVHIMQDKDLEENYHAQFIQRSLTQAGFESKILFGLDELR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHH
WDAAGQLIDADGRLVNCVWKTWAWETAIEQVREVSAEEYAAVPIRTGHPQNEVRLIDVLL
HHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPVLWSLFPHHRYLLDTDFEVNDELAKTGYAVKPISGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEEECCCCCCC
NNIDLIGPQDELLDKTSGQFVDRKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCEEECCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
SSLVVKKESDIEPLIVLKDKA
CCEEEEECCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]