Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 238896492

GI number: 238896492

Start: 4471243

End: 4471962

Strand: Reverse

Name: trmB

Synonym: KP1_4672

Alternate gene names: 238896492

Gene position: 4471962-4471243 (Counterclockwise)

Preceding gene: 238896497

Following gene: 238896491

Centisome position: 85.2

GC content: 55.97

Gene sequence:

>720_bases
ATGAAAAACGACGTCATCTCTCCGGAATTCGATGAAAACGGTCGCCCGCTGCGCCGTATTCGTAGCTTCGTCCGTCGCCA
GGGCCGCCTGACGAAAGGGCAGCAGCATGCGCTGGATAACATTTGGCCGGTGATGGGCGTTGAATTCAACGATGCGCCGC
TCGATTTCGCCGCCCTGTTTGGCCGTGACGCGCCGGTCACCTTAGAAATTGGCTTCGGGATGGGCGCCTCGCTGGTGGCG
ATGGCGAAAGCCAAACCGGAACAGAATTTCCTCGGCATTGAAGTACACTCCCCGGGCGTTGGCGCTTGTCTGGCTTCTGC
GGAAGAAGAAGGCGTACAAAACCTGCGCGTAATGTGTCACGATGCGGTGGAAGTGCTGCATACCATGATCCCGGATAACT
CGCTGAATATGGTGCAACTGTTTTTCCCTGATCCGTGGCATAAAGCGCGTCATAATAAACGTCGTATCGTGCAGCCGCCT
TTCGCTGAGCTGGTGAAAAGCAAGTTGAAGCTCGGCGGCGTCTTCCATATGGCGACCGACTGGGAAGCCTATGCCGTGCA
TATGCTGGAAGTTATGTCCTCGCTGGAAGGCTATCGTAACCAGTCGGCAAGCAACGATTACGTACCGCGCCCGGAATCGC
GCCCGGTAACCAAATTTGAACAGCGTGGCCATCGTCTCGGCCACGGCGTATGGGACTTAATGTTCGAGAGGGTGAAATAA

Upstream 100 bases:

>100_bases
ATTGCAGCATAAGGGCGAGTGGCTGTAAACCCTTGCATCCGCCGGGTAACTTTGGATAATGCCCGTTTTCCGAACACATT
CACAAGCAGACCAAACTTTT

Downstream 100 bases:

>100_bases
TGGCCAAGAATCGTAGCCGTCGTCTGCGTAAAAAAATGCATATCGATGAGTTTCAGGAGTTAGGCTTCTCCGTCGCCTGG
CGTTTCCCGGAAGGGACCAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQQHALDNIWPVMGVEFNDAPLDFAALFGRDAPVTLEIGFGMGASLVA
MAKAKPEQNFLGIEVHSPGVGACLASAEEEGVQNLRVMCHDAVEVLHTMIPDNSLNMVQLFFPDPWHKARHNKRRIVQPP
FAELVKSKLKLGGVFHMATDWEAYAVHMLEVMSSLEGYRNQSASNDYVPRPESRPVTKFEQRGHRLGHGVWDLMFERVK

Sequences:

>Translated_239_residues
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQQHALDNIWPVMGVEFNDAPLDFAALFGRDAPVTLEIGFGMGASLVA
MAKAKPEQNFLGIEVHSPGVGACLASAEEEGVQNLRVMCHDAVEVLHTMIPDNSLNMVQLFFPDPWHKARHNKRRIVQPP
FAELVKSKLKLGGVFHMATDWEAYAVHMLEVMSSLEGYRNQSASNDYVPRPESRPVTKFEQRGHRLGHGVWDLMFERVK
>Mature_239_residues
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQQHALDNIWPVMGVEFNDAPLDFAALFGRDAPVTLEIGFGMGASLVA
MAKAKPEQNFLGIEVHSPGVGACLASAEEEGVQNLRVMCHDAVEVLHTMIPDNSLNMVQLFFPDPWHKARHNKRRIVQPP
FAELVKSKLKLGGVFHMATDWEAYAVHMLEVMSSLEGYRNQSASNDYVPRPESRPVTKFEQRGHRLGHGVWDLMFERVK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=239, Percent_Identity=88.7029288702929, Blast_Score=443, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_KLEP7 (A6TDW8)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001337019.1
- ProteinModelPortal:   A6TDW8
- SMR:   A6TDW8
- STRING:   A6TDW8
- GeneID:   5339743
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_03392
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27013; Mature: 27013

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 94-94 BINDING 121-121 BINDING 144-144 BINDING 148-148 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQQHALDNIWPVMGVEFNDAPLDFAALF
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHCCCCCCCCHHHHHHH
GRDAPVTLEIGFGMGASLVAMAKAKPEQNFLGIEVHSPGVGACLASAEEEGVQNLRVMCH
CCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DAVEVLHTMIPDNSLNMVQLFFPDPWHKARHNKRRIVQPPFAELVKSKLKLGGVFHMATD
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
WEAYAVHMLEVMSSLEGYRNQSASNDYVPRPESRPVTKFEQRGHRLGHGVWDLMFERVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQQHALDNIWPVMGVEFNDAPLDFAALF
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHCCCCCCCCHHHHHHH
GRDAPVTLEIGFGMGASLVAMAKAKPEQNFLGIEVHSPGVGACLASAEEEGVQNLRVMCH
CCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DAVEVLHTMIPDNSLNMVQLFFPDPWHKARHNKRRIVQPPFAELVKSKLKLGGVFHMATD
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
WEAYAVHMLEVMSSLEGYRNQSASNDYVPRPESRPVTKFEQRGHRLGHGVWDLMFERVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA