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The map label for this gene is cof [H]

Identifier: 238893395

GI number: 238893395

Start: 1235840

End: 1236787

Strand: Direct

Name: cof [H]

Synonym: KP1_1279

Alternate gene names: 238893395

Gene position: 1235840-1236787 (Clockwise)

Preceding gene: 238893392

Following gene: 238893397

Centisome position: 23.55

GC content: 56.65

Gene sequence:

>948_bases
ATGGGGGCCGTCGTCCACCGGGCCGCTGTAAACCCGGAACAATTAATAATATCTATTCACTATTACTTCCGTATATCCCA
GGTGATACTGCAACACAAGCGAAGCCCATCACACATCGGGGAATATACCATGGCAAAACTGGCGGCATTTGATATGGACG
GCACCCTGCTGATGCCGGACCATCGTCTTGGAGAAAAGACGCTTTCCGCGCTGAAACGGCTGCGTGAGCGCGATATCACC
TTGACCTTCGCCACCGGCCGCCATGTGCTGGAGATGCACCATGTGATTGGCGAATTTTCCCTTGATGCATTTCTGATCAC
CGGCAACGGGACGCGCATCCATTCGCTGGAAGGGGAGGAGCTTTATCGCCAGGATCTCGCTCCCGAAGCGGCGGAAGCGG
TGCTGCACGGCAAATGGGATACCCAGGCCAGCATGCACGTCTTTAACGATGGCGGCTGGTTCACCGGCCAGGCAAGGCCT
GAATTGTTGCAAGCGCATGTATTCAGCGGCTTTCACTATCAACTGTGCGATCTGAAGCGCATGTCGGCGCAGCATGTCAC
CAAGATCTGTTTCTGCGGCGATCATGACGATTTGCGCCGTCTGCGGATCCAGCTGAATGAGACGCTGGGCGATCGCGCAT
TTCTCTGCTTCTCGGCAATGGACTGTCTGGAGGTGCTGCCGGTTGGCTGCAATAAAGGCGCGGCGCTGGCGGTGCTCAGC
CAGCATTTAGGCTTCACCCTGCAGGAGTGCATGGCCTTCGGCGATGCGATGAACGACCGCGAGATGCTGGGCAGCGTTGG
GCGGGGGTTCATCATGGGCAACGCGATGCCGCAGCTGAAAGCCGAGCTGCCGCATCTGCCGGTAATTGGCGACTGCCGGA
ATCAGGCAGTCTCCCATTTTTTAACTCATTGGCTGGATAATCCTGATCTTCCTTATTCCCCCGAATAG

Upstream 100 bases:

>100_bases
AAAACAGCGCTCCGCCAGTTCGCCGACGGTGACCTGCTGCGGCGCCCCGGCTGAGGGTTGCCATAGGCGCTGGTATTGAT
TTAAACGGTGTAAGAGTCGC

Downstream 100 bases:

>100_bases
AGAGACCCTTCCAGCAAGCCAGATAGGCTTCTATCTGGCTTTTTTTATCGCCTTTCGACCAGACCGGCGATCTCCGCCTG
CCAGGGCGCAATATCGCCGA

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MGAVVHRAAVNPEQLIISIHYYFRISQVILQHKRSPSHIGEYTMAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDIT
LTFATGRHVLEMHHVIGEFSLDAFLITGNGTRIHSLEGEELYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARP
ELLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLS
QHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELPHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE

Sequences:

>Translated_315_residues
MGAVVHRAAVNPEQLIISIHYYFRISQVILQHKRSPSHIGEYTMAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDIT
LTFATGRHVLEMHHVIGEFSLDAFLITGNGTRIHSLEGEELYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARP
ELLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLS
QHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELPHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE
>Mature_314_residues
GAVVHRAAVNPEQLIISIHYYFRISQVILQHKRSPSHIGEYTMAKLAAFDMDGTLLMPDHRLGEKTLSALKRLRERDITL
TFATGRHVLEMHHVIGEFSLDAFLITGNGTRIHSLEGEELYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPE
LLQAHVFSGFHYQLCDLKRMSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLSQ
HLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELPHLPVIGDCRNQAVSHFLTHWLDNPDLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=77.5735294117647, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=37.3076923076923, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786982, Length=277, Percent_Identity=27.4368231046931, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367265, Length=242, Percent_Identity=24.7933884297521, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 35316; Mature: 35185

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVVHRAAVNPEQLIISIHYYFRISQVILQHKRSPSHIGEYTMAKLAAFDMDGTLLMPD
CCCCHHHHCCCHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCC
HRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDAFLITGNGTRIHSLEGEE
CCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHH
LYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELLQAHVFSGFHYQLCDLKR
HHHHHCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHHHHHHHHCCCHHHHHHHHH
MSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLS
HHHHHHHEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELPHLPVIGDCRNQAVSHF
HHHCHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCHHHHHHHHH
LTHWLDNPDLPYSPE
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
GAVVHRAAVNPEQLIISIHYYFRISQVILQHKRSPSHIGEYTMAKLAAFDMDGTLLMPD
CCCHHHHCCCHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCC
HRLGEKTLSALKRLRERDITLTFATGRHVLEMHHVIGEFSLDAFLITGNGTRIHSLEGEE
CCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHH
LYRQDLAPEAAEAVLHGKWDTQASMHVFNDGGWFTGQARPELLQAHVFSGFHYQLCDLKR
HHHHHCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHHHHHHHHCCCHHHHHHHHH
MSAQHVTKICFCGDHDDLRRLRIQLNETLGDRAFLCFSAMDCLEVLPVGCNKGAALAVLS
HHHHHHHEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGNAMPQLKAELPHLPVIGDCRNQAVSHF
HHHCHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCHHHHHHHHH
LTHWLDNPDLPYSPE
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA