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The map label for this gene is queA [H]

Identifier: 238893341

GI number: 238893341

Start: 1182414

End: 1183478

Strand: Direct

Name: queA [H]

Synonym: KP1_1221

Alternate gene names: 238893341

Gene position: 1182414-1183478 (Clockwise)

Preceding gene: 238893340

Following gene: 238893342

Centisome position: 22.53

GC content: 60.47

Gene sequence:

>1065_bases
ATGCGCGTTACCGATTTTGCTTTTGAACTACCCGAATCTCTGATCGCCCACTATCCGATGCCTGAGCGCAGTAGCTGTCG
CTTGCTCTCCCTGGACGGGCCGACGGGCGCGCTGACGCATGGCACCTTCACCGACATCCTCGATAAGCTCAATCCCGGCG
ATCTGCTGGTGTTTAACAACACCCGGGTGATCCCGGCGCGTCTGTTCGGCCGCAAGGCCAGCGGCGGCAAGATTGAAGTG
CTGGTGGAGCGGATGCTGGATGATAAGCGCATCCTGGCCCATATTCGCGCCTCAAAAGCGCCGAAGCCGGGCGCCGAGCT
GCTGCTGGGCGATGACGAGAGCATTAAGGCCACGATGCTGGCGCGCCACGGCGCGCTGTTTGAAGTGGAGTTTAACGACG
AGCGCCCGGTGCTGGAGATCCTTAACGGGATCGGCCATATGCCGCTGCCGCCCTATATTGACCGCCCGGACGAAGACGCC
GACCGCGAGCTCTATCAAACCGTCTATGGCACCAGGCCGGGCGCGGTAGCCGCCCCGACCGCCGGTTTGCATTTCGACGA
ACCGCTGCTGGACAAACTGCGCGCCAAAGGCGTTGAGATGGCGTTTGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGC
CGGTGCGCGTCGACAGCATTGAAGAGCACACCATGCACTCCGAATATGCCGAAGTGCCGCAGGAGGTGGTCGATGCGGTG
CTGGCGGCGAAAGCGCGCGGCAACCGCGTGATCGCGGTGGGCACCACCTCGGTACGTTCGCTGGAGAGCGCGGCGCAGGC
GGCGAAAGACGCGCTGATCGCGCCGTTCTTCGACGATACGCAGATTTTCATCTACCCGGGTTATCAGTACCAGGTGATCG
ATGCGCTGGTGACTAACTTCCATCTCCCTGAATCCACACTGATTATGCTGGTGTCGGCGTTTGCGGGTTATCAGCACACC
ATGAACGCCTATAAGGTGGCGGTTGAGCAGAAATATCGCTTTTTTAGCTACGGGGATGCGATGTTTATCACGTACAATCC
GCAGGCTATTTCTGAGCGTCCCTGA

Upstream 100 bases:

>100_bases
TGGAATATAGATTATGTCATTCATCCGGAGGGGTAAAGGTTGCAGCCAGACCGCCCCGGCACTAGACTACCCGCCTCTTT
ATTCAGTCTGAGTTACCGTC

Downstream 100 bases:

>100_bases
AAAGGCCGGTACGCCGGCTTGTAGACGCCATAACGCATTTTTTAGCTACGGAATCATTCCGTGGCGAGTATGAAAACGTC
GGACTGTTTTTCTGACGCAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRVTDFAFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDILDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESIKATMLARHGALFEVEFNDERPVLEILNGIGHMPLPPYIDRPDEDA
DRELYQTVYGTRPGAVAAPTAGLHFDEPLLDKLRAKGVEMAFVTLHVGAGTFQPVRVDSIEEHTMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKDALIAPFFDDTQIFIYPGYQYQVIDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKVAVEQKYRFFSYGDAMFITYNPQAISERP

Sequences:

>Translated_354_residues
MRVTDFAFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDILDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESIKATMLARHGALFEVEFNDERPVLEILNGIGHMPLPPYIDRPDEDA
DRELYQTVYGTRPGAVAAPTAGLHFDEPLLDKLRAKGVEMAFVTLHVGAGTFQPVRVDSIEEHTMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKDALIAPFFDDTQIFIYPGYQYQVIDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKVAVEQKYRFFSYGDAMFITYNPQAISERP
>Mature_354_residues
MRVTDFAFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDILDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESIKATMLARHGALFEVEFNDERPVLEILNGIGHMPLPPYIDRPDEDA
DRELYQTVYGTRPGAVAAPTAGLHFDEPLLDKLRAKGVEMAFVTLHVGAGTFQPVRVDSIEEHTMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKDALIAPFFDDTQIFIYPGYQYQVIDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKVAVEQKYRFFSYGDAMFITYNPQAISERP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=93.2011331444759, Blast_Score=682, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39134; Mature: 39134

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTDFAFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDILDKLNPGDLLVFNN
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESIKATML
CEEEEHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHEE
ARHGALFEVEFNDERPVLEILNGIGHMPLPPYIDRPDEDADRELYQTVYGTRPGAVAAPT
ECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
AGLHFDEPLLDKLRAKGVEMAFVTLHVGAGTFQPVRVDSIEEHTMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKDALIAPFFDDTQIFIYPGYQYQVIDALVTNF
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCEEECEECCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKVAVEQKYRFFSYGDAMFITYNPQAISERP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEEECCEEEEEECCHHCCCCC
>Mature Secondary Structure
MRVTDFAFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDILDKLNPGDLLVFNN
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESIKATML
CEEEEHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHEE
ARHGALFEVEFNDERPVLEILNGIGHMPLPPYIDRPDEDADRELYQTVYGTRPGAVAAPT
ECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
AGLHFDEPLLDKLRAKGVEMAFVTLHVGAGTFQPVRVDSIEEHTMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKDALIAPFFDDTQIFIYPGYQYQVIDALVTNF
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCEEECEECCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKVAVEQKYRFFSYGDAMFITYNPQAISERP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEEECCEEEEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA