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The map label for this gene is leuC [H]

Identifier: 238893054

GI number: 238893054

Start: 876493

End: 877893

Strand: Reverse

Name: leuC [H]

Synonym: KP1_0894

Alternate gene names: 238893054

Gene position: 877893-876493 (Counterclockwise)

Preceding gene: 238893055

Following gene: 238893053

Centisome position: 16.73

GC content: 59.6

Gene sequence:

>1401_bases
ATGGCGAAGACGTTATACGAAAAATTATTTGATGCGCACGTAGTGTATGAGGCGCAGAACGAAACCCCGCTGCTGTATAT
CGACCGCCATCTGGTGCACGAAGTCACCTCTCCGCAGGCGTTTGACGGTCTGCGCGCCCATGGCCGCCAGGTGCGCCAGC
CGGGTAAAACCTTCGCTACCATGGATCATAACGTCTCGACGCAGACCAAAGACATTAACGCGTCCGGTGAGATGGCCCGC
ATTCAGATGCAGGAGCTGATTAAGAACTGTAAAGAGTTCGGCGTTGAACTGTACGACCTGAACCACCCGTATCAGGGCAT
CGTCCACGTCATGGGCCCGGAACAAGGGGTTACCCTGCCGGGGATGACGATCGTGTGCGGTGACTCCCATACCGCCACCC
ACGGCGCGTTCGGTGCGCTGGCGTTCGGTATCGGCACCTCCGAAGTGGAGCATGTGCTGGCGACCCAGACCCTGAAACAG
GGCCGCGCGAAGACCATGAAAATCGAAGTCCAGGGCAAAGCGGCGCCGGGCATTACCGCCAAAGATATCGTGCTGGCCAT
CATTGGTAAAACCGGTAGCGCCGGCGGCACCGGCCATGTGGTTGAGTTCTGCGGCGAAGCGATCCGCGATCTGAGCATGG
AAGGCCGTATGACCCTGTGCAACATGGCGATCGAGATGGGCGCCAAAGCGGGCCTGGTCGCGCCGGACGAAACCACCTTC
AATTACGTACGCGGCCGTCTGCATGCGCCGAAAGGCAAAGATTTTGACGATGCCGTAGCGTACTGGAAAACCCTGAAAAC
CGACGACGGCGCGACGTTTGATACCGTGGTAACGCTGCAGGCGGCGGAAATTGCCCCGCAGGTGACCTGGGGCACCAACC
CGGGCCAGGTGATCTCGGTGACGGATAACATCCCCGATCCAGCGTCGTTCAGCGATCCGGTGGAACGCGCCAGCGCCGAA
AAAGCGCTGGCCTATATGGGACTGAAATCCGGGATCCCGCTGACGGAAGTCGCTATCGATAAAGTGTTTATCGGCTCCTG
CACCAACTCGCGCATCGAAGATTTACGCGCCGCGGCGGAAATCGCTAAAGGGCGTAAAGTGGCGCCGGGCGTCCAGGCGC
TGGTGGTGCCGGGTTCTGGTCCGGTGAAAGCGCAGGCGGAAGCGGAAGGTCTGGATAAGATCTTTATCGAAGCCGGCTTT
GAGTGGCGCTTACCTGGTTGCTCTATGTGTCTGGCAATGAATAATGACCGCCTGAATCCGGGCGAACGCTGTGCCTCAAC
CAGCAACCGTAACTTTGAAGGCCGTCAGGGCCGCGGCGGACGCACGCACCTGGTGAGCCCGGCAATGGCCGCGGCGGCAG
CCGTTACCGGCCATTTCGCCGATATCCGTAATCTGAAGTAA

Upstream 100 bases:

>100_bases
AAGGGGTTCGAACCGGCGACTTAGCGCGCGGCACGGCGGCGGTCAGCACTGATGAAATGGGCGATATTATTGCCCGCTAT
GTCGCAGAAGGGGTGTAATC

Downstream 100 bases:

>100_bases
GGAGACCATCATGGCAGAGAAATTTACCCAACATACCGGCCTGGTGGTTCCGCTGGATGCTGCAAACGTCGATACCGACG
CGATCATCCCTAAGCAATTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKLFDAHVVYEAQNETPLLYIDRHLVHEVTSPQAFDGLRAHGRQVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVRGRLHAPKGKDFDDAVAYWKTLKTDDGATFDTVVTLQAAEIAPQVTWGTNPGQVISVTDNIPDPASFSDPVERASAE
KALAYMGLKSGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNLK

Sequences:

>Translated_466_residues
MAKTLYEKLFDAHVVYEAQNETPLLYIDRHLVHEVTSPQAFDGLRAHGRQVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVRGRLHAPKGKDFDDAVAYWKTLKTDDGATFDTVVTLQAAEIAPQVTWGTNPGQVISVTDNIPDPASFSDPVERASAE
KALAYMGLKSGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNLK
>Mature_465_residues
AKTLYEKLFDAHVVYEAQNETPLLYIDRHLVHEVTSPQAFDGLRAHGRQVRQPGKTFATMDHNVSTQTKDINASGEMARI
QMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQG
RAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFN
YVRGRLHAPKGKDFDDAVAYWKTLKTDDGATFDTVVTLQAAEIAPQVTWGTNPGQVISVTDNIPDPASFSDPVERASAEK
ALAYMGLKSGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFE
WRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=26.7676767676768, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4501867, Length=410, Percent_Identity=25.609756097561, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=26.6318537859008, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=97.4248927038627, Blast_Score=932, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=27.9778393351801, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI2367097, Length=356, Percent_Identity=25.8426966292135, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=411, Percent_Identity=28.7104622871046, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=28.7104622871046, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=30.3921568627451, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=451, Percent_Identity=27.0509977827051, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.2972399150743, Blast_Score=589, Evalue=1e-169,
Organism=Saccharomyces cerevisiae, GI6320440, Length=425, Percent_Identity=25.4117647058824, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=402, Percent_Identity=27.6119402985075, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=29.1553133514986, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281365315, Length=474, Percent_Identity=24.6835443037975, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17864292, Length=474, Percent_Identity=24.6835443037975, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161076999, Length=409, Percent_Identity=24.9388753056235, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=409, Percent_Identity=26.161369193154, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645686, Length=451, Percent_Identity=26.1640798226164, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137564, Length=455, Percent_Identity=25.7142857142857, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49829; Mature: 49698

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDAHVVYEAQNETPLLYIDRHLVHEVTSPQAFDGLRAHGRQVRQPGKTFAT
CCHHHHHHHHCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVRGRLHAPKGKDFDDAVAYWKTLKTDDGATFDTVVTLQAAEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHCCEEEECCCCCEEEEE
TDNIPDPASFSDPVERASAEKALAYMGLKSGIPLTEVAIDKVFIGSCTNSRIEDLRAAAE
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHH
IAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNLK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKTLYEKLFDAHVVYEAQNETPLLYIDRHLVHEVTSPQAFDGLRAHGRQVRQPGKTFAT
CHHHHHHHHCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVRGRLHAPKGKDFDDAVAYWKTLKTDDGATFDTVVTLQAAEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHCCEEEECCCCCEEEEE
TDNIPDPASFSDPVERASAEKALAYMGLKSGIPLTEVAIDKVFIGSCTNSRIEDLRAAAE
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHH
IAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNLK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA