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The map label for this gene is polB [H]

Identifier: 238893035

GI number: 238893035

Start: 852507

End: 854864

Strand: Reverse

Name: polB [H]

Synonym: KP1_0872

Alternate gene names: 238893035

Gene position: 854864-852507 (Counterclockwise)

Preceding gene: 238893036

Following gene: 238893034

Centisome position: 16.29

GC content: 61.79

Gene sequence:

>2358_bases
GTGACCCAGCCACGAGCAGGATTTTTACTCACCCGTCACTGGCGGGACACTCCGCAGGGCACCGAGCTCTCCTTCTGGCT
GGCCACCGACGACGGTCCGCTGCAGGTCACCCTCCCGCCGCAGGAGTCCGTCGCCTTTATTCCCGAAGCGCAGCGGGCGC
AGGCTGAACGGCTGCTGCAGGGGGAGAAAGGGCTCCGCTTCGCCCCGCTGGCCCTGAAAGATTTCCACCGTCAGCCGATT
GTTGGCCTCTACTGCCGCGCCCATCGCCAGTTGATGCGTCTGGAGAAGATGCTCCGCGACAGCGGCGTCACCGTTTACGA
AGGCGATATCCGCCCGCCGGAGCGCTATCTGATGGAGCGCTTTATCACCGCCCCGGTGTGGGTGGAAGGCGAAACGCGCG
GCTCGCAGCTGGTCAACGCGCGCATGAAGCCGAACCCTGACTATCGTCCGCCGCTGAAATGGGTGTCGCTGGATATCGAA
ACCAGCCGCCACGGCGAGCTGTACTGCATCGGTCTGGAAGGCTGCGGCCAGCGGGTGGTCTACATGCTGGGCCCCGAACC
GGAAACGCCGCCAGACGTCGACTTCGAGCTGGTCTTCATCGCCAGCCGTCCCCTGCTGCTGGAAAAGCTTAACGCCTGGT
TTGCCGAACACGACCCCGACGTGCTGATCGGCTGGAACGTAGTGCAGTTCGATCTGCGCGTTCTGCAAAAGCACGCCGAA
CGCTATCGTATCCCGCTGCGGCTCGGCCGCGGCAACAGCGAGCTGGAGTGGCGTGAGCACGGCTTTAAGAACGGCGTCTA
TTTCGCCCAGGCCAACGGGCGGCTGATTATCGACGGCATCGACGCGCTAAAATCGGCGTTCTGGAATTTCTCCTCCTTTT
CGCTGGAGGCGGTGGCCCGCGAGCTGCTGGGAGAAGGCAAAGCCATCGACAACCCATGGGATCGGATGGATGAGATCGAG
CGCCGGTTTCATGAAGACAAACCGGCGCTGGCCATCTATAACCTGCAGGATTGCGAGCTGGTGACGCGGATCTTCCACAA
AACGGAGATCATGCCGTTTTTGCTCGAACGAGCGACGGTCAACGGTCTGCCCGCCGACCGCCACGGCGGCTCGGTCGCCG
CCTTCAGCCATCTCTATTTCCCGCGCATGCACCGCCTGGGCTACGTGGCGCCGAATCTCGGCGACGTACCGCCGCAGGCG
AGCCCCGGCGGCTACGTGATGGACTCCCGCCCCGGCCTGTATGACTCGGTGCTGGTGCTGGACTATAAAAGCCTGTATCC
GTCGATTATTCGTACCTTTTTAATCGATCCGGTCGGCCTGGTTGAAGGGCTGGCGCAACCCGATGATCAGCACAGTATTG
AGGGCTTCCTCGGCGCCCGCTTCTCGCGAGATAAGCACTGCCTGCCGGGGATTGTCAGCCAGATCTGGCACGGCCGGGAT
GAAGCCAAACGCCAGCACAATAAGCCGCTCTCCCAGGCGCTGAAGATCATTATGAACGCCTTTTACGGCGTGCTGGGGAC
CAGCGCCTGCCGCTTCTTCGACCCGCGTCTCGCCTCGTCGATCACCATGCGCGGCCATGCGATCATGCGCCAGACCAAAG
CGTTAATTGAAGCCAAAGGCTATGACGTTATCTACGGCGATACCGACTCCACCTTCGTCTGGCTGAAGCGCCCGCACAGC
GAAGCCCAGGCGGCGGAGATCGGCCGCGAGCTGGTCAGCGATGTCAACGCCTGGTGGGCGCAGGAGCTTAGCAAAAGCCA
GCTTACCAGCGCCCTGGAGCTGGAGTATGAAACCCACTTCTGCCGCTTTTTAATGCCGACGATCCGCGGCGCCGATACCG
GCAGCAAGAAGCGCTATGCCGGGATGATTCAGGAGGGCGATGCCCAGCGCATGGTGTTTAAGGGGCTGGAAACGGTGCGT
ACCGACTGGACGCCGCTGGCCCAGCAGTTTCAACAGGAGTTATACCTGCGCATCTTCCGCAACCAGCCGTATCAGGATTA
TGTGCGGGAGACTATCGCCAGGCTGATGAACGGCGAGCTCGACGAACAGCTGGTTTATCGCAAACGCCTGCGCCGGCCGC
TGGCGGAGTATCAGCGCAACGTACCGCCGCACGTGCGCGCCGCCCGGCTTGCCGATGAACACAACCTGAAGCTTGGGCGG
GCGCAGCAGTATCAGCAGCGCGGCACCATCAAATACGTCTGGACCACCAGCGGCCCGGAGCCGGCGGATTACCAGCAGTC
GCCGCTCGATTACGATCACTATTTAACAAAGCAGTTGCAGCCGGTGGCGGAAGGGATCCTGCCCTTCGTCAACGATGACT
TTGCTACAATAGTGACAGGACAGTTGGGGCTATTTTGA

Upstream 100 bases:

>100_bases
ATCCGGGGCAACATTAGCGCCGAACACCTGTATAAAACCCCAGCACCCTTCATGAAACCAGGCTATAATCAGGCCTGGTT
TTTTATCGGCGAGAATACCC

Downstream 100 bases:

>100_bases
TTTTTTGTCACCCATTCCACGGGCACGAAAGCCTAAAAATGAAGCGTGAGGAGAACGTGACGAACGTTCTGCCATCCAGT
AATATAGCGCCCTTTCCATT

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 785; Mature: 784

Protein sequence:

>785_residues
MTQPRAGFLLTRHWRDTPQGTELSFWLATDDGPLQVTLPPQESVAFIPEAQRAQAERLLQGEKGLRFAPLALKDFHRQPI
VGLYCRAHRQLMRLEKMLRDSGVTVYEGDIRPPERYLMERFITAPVWVEGETRGSQLVNARMKPNPDYRPPLKWVSLDIE
TSRHGELYCIGLEGCGQRVVYMLGPEPETPPDVDFELVFIASRPLLLEKLNAWFAEHDPDVLIGWNVVQFDLRVLQKHAE
RYRIPLRLGRGNSELEWREHGFKNGVYFAQANGRLIIDGIDALKSAFWNFSSFSLEAVARELLGEGKAIDNPWDRMDEIE
RRFHEDKPALAIYNLQDCELVTRIFHKTEIMPFLLERATVNGLPADRHGGSVAAFSHLYFPRMHRLGYVAPNLGDVPPQA
SPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGLAQPDDQHSIEGFLGARFSRDKHCLPGIVSQIWHGRD
EAKRQHNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITMRGHAIMRQTKALIEAKGYDVIYGDTDSTFVWLKRPHS
EAQAAEIGRELVSDVNAWWAQELSKSQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGMIQEGDAQRMVFKGLETVR
TDWTPLAQQFQQELYLRIFRNQPYQDYVRETIARLMNGELDEQLVYRKRLRRPLAEYQRNVPPHVRAARLADEHNLKLGR
AQQYQQRGTIKYVWTTSGPEPADYQQSPLDYDHYLTKQLQPVAEGILPFVNDDFATIVTGQLGLF

Sequences:

>Translated_785_residues
MTQPRAGFLLTRHWRDTPQGTELSFWLATDDGPLQVTLPPQESVAFIPEAQRAQAERLLQGEKGLRFAPLALKDFHRQPI
VGLYCRAHRQLMRLEKMLRDSGVTVYEGDIRPPERYLMERFITAPVWVEGETRGSQLVNARMKPNPDYRPPLKWVSLDIE
TSRHGELYCIGLEGCGQRVVYMLGPEPETPPDVDFELVFIASRPLLLEKLNAWFAEHDPDVLIGWNVVQFDLRVLQKHAE
RYRIPLRLGRGNSELEWREHGFKNGVYFAQANGRLIIDGIDALKSAFWNFSSFSLEAVARELLGEGKAIDNPWDRMDEIE
RRFHEDKPALAIYNLQDCELVTRIFHKTEIMPFLLERATVNGLPADRHGGSVAAFSHLYFPRMHRLGYVAPNLGDVPPQA
SPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGLAQPDDQHSIEGFLGARFSRDKHCLPGIVSQIWHGRD
EAKRQHNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITMRGHAIMRQTKALIEAKGYDVIYGDTDSTFVWLKRPHS
EAQAAEIGRELVSDVNAWWAQELSKSQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGMIQEGDAQRMVFKGLETVR
TDWTPLAQQFQQELYLRIFRNQPYQDYVRETIARLMNGELDEQLVYRKRLRRPLAEYQRNVPPHVRAARLADEHNLKLGR
AQQYQQRGTIKYVWTTSGPEPADYQQSPLDYDHYLTKQLQPVAEGILPFVNDDFATIVTGQLGLF
>Mature_784_residues
TQPRAGFLLTRHWRDTPQGTELSFWLATDDGPLQVTLPPQESVAFIPEAQRAQAERLLQGEKGLRFAPLALKDFHRQPIV
GLYCRAHRQLMRLEKMLRDSGVTVYEGDIRPPERYLMERFITAPVWVEGETRGSQLVNARMKPNPDYRPPLKWVSLDIET
SRHGELYCIGLEGCGQRVVYMLGPEPETPPDVDFELVFIASRPLLLEKLNAWFAEHDPDVLIGWNVVQFDLRVLQKHAER
YRIPLRLGRGNSELEWREHGFKNGVYFAQANGRLIIDGIDALKSAFWNFSSFSLEAVARELLGEGKAIDNPWDRMDEIER
RFHEDKPALAIYNLQDCELVTRIFHKTEIMPFLLERATVNGLPADRHGGSVAAFSHLYFPRMHRLGYVAPNLGDVPPQAS
PGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGLAQPDDQHSIEGFLGARFSRDKHCLPGIVSQIWHGRDE
AKRQHNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITMRGHAIMRQTKALIEAKGYDVIYGDTDSTFVWLKRPHSE
AQAAEIGRELVSDVNAWWAQELSKSQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGMIQEGDAQRMVFKGLETVRT
DWTPLAQQFQQELYLRIFRNQPYQDYVRETIARLMNGELDEQLVYRKRLRRPLAEYQRNVPPHVRAARLADEHNLKLGRA
QQYQQRGTIKYVWTTSGPEPADYQQSPLDYDHYLTKQLQPVAEGILPFVNDDFATIVTGQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=737, Percent_Identity=26.1872455902307, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI106507301, Length=806, Percent_Identity=23.697270471464, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1786246, Length=781, Percent_Identity=83.9948783610755, Blast_Score=1384, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=702, Percent_Identity=26.7806267806268, Blast_Score=145, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI32565317, Length=790, Percent_Identity=22.9113924050633, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320101, Length=632, Percent_Identity=25.6329113924051, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324227, Length=405, Percent_Identity=23.9506172839506, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648780, Length=670, Percent_Identity=23.7313432835821, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24664937, Length=672, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 89892; Mature: 89761

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPRAGFLLTRHWRDTPQGTELSFWLATDDGPLQVTLPPQESVAFIPEAQRAQAERLLQ
CCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCEEECCCHHHHHHHHHHC
GEKGLRFAPLALKDFHRQPIVGLYCRAHRQLMRLEKMLRDSGVTVYEGDIRPPERYLMER
CCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
FITAPVWVEGETRGSQLVNARMKPNPDYRPPLKWVSLDIETSRHGELYCIGLEGCGQRVV
HHCCCEEECCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCEEE
YMLGPEPETPPDVDFELVFIASRPLLLEKLNAWFAEHDPDVLIGWNVVQFDLRVLQKHAE
EEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
RYRIPLRLGRGNSELEWREHGFKNGVYFAQANGRLIIDGIDALKSAFWNFSSFSLEAVAR
HEECCEEECCCCCCCHHHHCCCCCCEEEEECCCEEEEECHHHHHHHHCCCCCCCHHHHHH
ELLGEGKAIDNPWDRMDEIERRFHEDKPALAIYNLQDCELVTRIFHKTEIMPFLLERATV
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
NGLPADRHGGSVAAFSHLYFPRMHRLGYVAPNLGDVPPQASPGGYVMDSRPGLYDSVLVL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHEE
DYKSLYPSIIRTFLIDPVGLVEGLAQPDDQHSIEGFLGARFSRDKHCLPGIVSQIWHGRD
EHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHH
EAKRQHNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITMRGHAIMRQTKALIEAKG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YDVIYGDTDSTFVWLKRPHSEAQAAEIGRELVSDVNAWWAQELSKSQLTSALELEYETHF
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRFLMPTIRGADTGSKKRYAGMIQEGDAQRMVFKGLETVRTDWTPLAQQFQQELYLRIFR
HHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
NQPYQDYVRETIARLMNGELDEQLVYRKRLRRPLAEYQRNVPPHVRAARLADEHNLKLGR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCH
AQQYQQRGTIKYVWTTSGPEPADYQQSPLDYDHYLTKQLQPVAEGILPFVNDDFATIVTG
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCHHHEEEC
QLGLF
CCCCC
>Mature Secondary Structure 
TQPRAGFLLTRHWRDTPQGTELSFWLATDDGPLQVTLPPQESVAFIPEAQRAQAERLLQ
CCCCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCEEECCCHHHHHHHHHHC
GEKGLRFAPLALKDFHRQPIVGLYCRAHRQLMRLEKMLRDSGVTVYEGDIRPPERYLMER
CCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
FITAPVWVEGETRGSQLVNARMKPNPDYRPPLKWVSLDIETSRHGELYCIGLEGCGQRVV
HHCCCEEECCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCEEE
YMLGPEPETPPDVDFELVFIASRPLLLEKLNAWFAEHDPDVLIGWNVVQFDLRVLQKHAE
EEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
RYRIPLRLGRGNSELEWREHGFKNGVYFAQANGRLIIDGIDALKSAFWNFSSFSLEAVAR
HEECCEEECCCCCCCHHHHCCCCCCEEEEECCCEEEEECHHHHHHHHCCCCCCCHHHHHH
ELLGEGKAIDNPWDRMDEIERRFHEDKPALAIYNLQDCELVTRIFHKTEIMPFLLERATV
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
NGLPADRHGGSVAAFSHLYFPRMHRLGYVAPNLGDVPPQASPGGYVMDSRPGLYDSVLVL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHEE
DYKSLYPSIIRTFLIDPVGLVEGLAQPDDQHSIEGFLGARFSRDKHCLPGIVSQIWHGRD
EHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHH
EAKRQHNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITMRGHAIMRQTKALIEAKG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YDVIYGDTDSTFVWLKRPHSEAQAAEIGRELVSDVNAWWAQELSKSQLTSALELEYETHF
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRFLMPTIRGADTGSKKRYAGMIQEGDAQRMVFKGLETVRTDWTPLAQQFQQELYLRIFR
HHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
NQPYQDYVRETIARLMNGELDEQLVYRKRLRRPLAEYQRNVPPHVRAARLADEHNLKLGR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCH
AQQYQQRGTIKYVWTTSGPEPADYQQSPLDYDHYLTKQLQPVAEGILPFVNDDFATIVTG
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCHHHEEEC
QLGLF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]