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The map label for this gene is hpaI [H]

Identifier: 238892914

GI number: 238892914

Start: 725173

End: 725970

Strand: Reverse

Name: hpaI [H]

Synonym: KP1_0737

Alternate gene names: 238892914

Gene position: 725970-725173 (Counterclockwise)

Preceding gene: 238892915

Following gene: 238892913

Centisome position: 13.83

GC content: 63.28

Gene sequence:

>798_bases
ATGAATAACGCCTTTAAAGACGCCTTAAAAGCGGGCCGTCCGCAAATCGGCCTGTGGCTTGGGCTGTGCAGCAGCTACAG
CGCCGAACTGCTGGCCGGCGCGGGCTTCGACTGGCTGCTGATCGACGGCGAACACGCGCCCAACAATGTACCAACGGTGC
TGACCCAGCTGCAGGCCATTGCGCCCTACCCCAGCCAGCCGGTGGTGCGTCCGTCGTGGAACGATCCGGTGCAGATCAAA
CAGCTGCTGGATGTCGGCGCGCAAACCCTGCTGGTGCCGATGGTGCAGAATGCCGAAGAGGCGCGGCTGGCGGTCAGAGC
CACCCGCTATCCGCCAGCCGGCATCCGCGGCGTCGGCAGCGCGCTGGCCCGCGCCTCACGCTGGAATCGCGTCCCGGATT
ATATCCACCAGGCCAATGACGCCATGTGCGTCCTGGTACAGATTGAGACTCGCGAAGCGCTGAAAAATCTGCCGCAGATC
CTCGATGTCGACGGGGTAGATGGTGTGTTTATCGGCCCGGCGGATCTCAGCGCTGATATGGGCCACGGCGGCAATCCGCA
GCACCCGGAAGTGCAGGCCGCCATTGAAGATGCCATCCAGCAAATCCGTCAGGCCGGCAAGGCGCCGGGGATCCTGATGG
CCAATGAACAGCTGGCGAAACGCTATCTCGAACTGGGCGCGCTGTTCGTCGCCGTCGGCGTCGATACCACCCTGCTGGCC
CGCGGCGCAGAAGCGCTGGCGGCCCGGTTTACCCACACCGCAACCACCACAACCGATAACAATAAATCCGTCTATTGA

Upstream 100 bases:

>100_bases
TCCTTCACCCGCCCGGTCCCGGCGCGCAAGGGCGATACCTTCCACGTCGACTACGGCAACATGGGCGCCATCAGCTGCCG
GTTTGTCTAGGAGAAACACC

Downstream 100 bases:

>100_bases
ACGGATAATGTGGAGCGCAACATGACTACCTCTACCCTGCAACACAATGATAATAAAGCCGTTGAAGTAGAAAACCGGGT
GATCAAAAAGCTGTTCCGCC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNNAFKDALKAGRPQIGLWLGLCSSYSAELLAGAGFDWLLIDGEHAPNNVPTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLVPMVQNAEEARLAVRATRYPPAGIRGVGSALARASRWNRVPDYIHQANDAMCVLVQIETREALKNLPQI
LDVDGVDGVFIGPADLSADMGHGGNPQHPEVQAAIEDAIQQIRQAGKAPGILMANEQLAKRYLELGALFVAVGVDTTLLA
RGAEALAARFTHTATTTTDNNKSVY

Sequences:

>Translated_265_residues
MNNAFKDALKAGRPQIGLWLGLCSSYSAELLAGAGFDWLLIDGEHAPNNVPTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLVPMVQNAEEARLAVRATRYPPAGIRGVGSALARASRWNRVPDYIHQANDAMCVLVQIETREALKNLPQI
LDVDGVDGVFIGPADLSADMGHGGNPQHPEVQAAIEDAIQQIRQAGKAPGILMANEQLAKRYLELGALFVAVGVDTTLLA
RGAEALAARFTHTATTTTDNNKSVY
>Mature_265_residues
MNNAFKDALKAGRPQIGLWLGLCSSYSAELLAGAGFDWLLIDGEHAPNNVPTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLVPMVQNAEEARLAVRATRYPPAGIRGVGSALARASRWNRVPDYIHQANDAMCVLVQIETREALKNLPQI
LDVDGVDGVFIGPADLSADMGHGGNPQHPEVQAAIEDAIQQIRQAGKAPGILMANEQLAKRYLELGALFVAVGVDTTLLA
RGAEALAARFTHTATTTTDNNKSVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=56, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=48.7903225806452, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28401; Mature: 28401

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAFKDALKAGRPQIGLWLGLCSSYSAELLAGAGFDWLLIDGEHAPNNVPTVLTQLQAI
CCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLVPMVQNAEEARLAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
ALARASRWNRVPDYIHQANDAMCVLVQIETREALKNLPQILDVDGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GHGGNPQHPEVQAAIEDAIQQIRQAGKAPGILMANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFTHTATTTTDNNKSVY
HHHHHHHHHHHHCEEECCCCCCCCC
>Mature Secondary Structure
MNNAFKDALKAGRPQIGLWLGLCSSYSAELLAGAGFDWLLIDGEHAPNNVPTVLTQLQAI
CCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLVPMVQNAEEARLAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
ALARASRWNRVPDYIHQANDAMCVLVQIETREALKNLPQILDVDGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GHGGNPQHPEVQAAIEDAIQQIRQAGKAPGILMANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFTHTATTTTDNNKSVY
HHHHHHHHHHHHCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA