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The map label for this gene is fosA [H]

Identifier: 238892888

GI number: 238892888

Start: 699857

End: 700276

Strand: Direct

Name: fosA [H]

Synonym: KP1_0708

Alternate gene names: 238892888

Gene position: 699857-700276 (Clockwise)

Preceding gene: 238892887

Following gene: 238892890

Centisome position: 13.33

GC content: 61.67

Gene sequence:

>420_bases
ATGCTGAGTGGACTGAATCACCTGACCCTGGCAGTCAGCCAGCTGGCGCCGAGCGTGGCGTTTTATCAGCAGCTGCTGGG
CATGACGCTGCATGCCCGCTGGGACAGCGGGGCTTATCTCTCCTGCGGCGATTTGTGGCTGTGCCTGTCGCTGGATCCGC
AGCGGCGCGTTACTCCGCCGGAGGAGAGCGACTACACCCATTATGCGTTTAGCATTAGCGAAGCCGATTTTGCTAGCTTC
GCCGCCCGCCTTGAGGCTGCCGGCGTGGCGATCTGGAAGCTGAACCGTAGCGAAGGCGCTTCGCACTATTTCCTCGACCC
CGATGGCCACAAGCTGGAGCTGCACGTCGGCAGTCTCGCCCAGCGTCTGGCCGCCTGCCGCGAACAGCCGTATAAGGGGA
TGGTGTTTTTTGATCAGTGA

Upstream 100 bases:

>100_bases
CCGGCTTTGAGATGCTGATGCTGTGGCACGAGCGCTGGCACCGCGACCCGGCGCATCAATGGCTGCGTCAGTTCATTGTG
AATTCACTGGAGGAACAGAC

Downstream 100 bases:

>100_bases
AGCTGCTTCGCAGACGTTAGATCACCCGTAGCCCCGGTAAGCGCAGCGCCACCGGGGAGAACAGGTTAAATTCACTCCAT
CGCCACCTTCACCGCTTCTC

Product: putative glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Fosfomycin resistance protein [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MLSGLNHLTLAVSQLAPSVAFYQQLLGMTLHARWDSGAYLSCGDLWLCLSLDPQRRVTPPEESDYTHYAFSISEADFASF
AARLEAAGVAIWKLNRSEGASHYFLDPDGHKLELHVGSLAQRLAACREQPYKGMVFFDQ

Sequences:

>Translated_139_residues
MLSGLNHLTLAVSQLAPSVAFYQQLLGMTLHARWDSGAYLSCGDLWLCLSLDPQRRVTPPEESDYTHYAFSISEADFASF
AARLEAAGVAIWKLNRSEGASHYFLDPDGHKLELHVGSLAQRLAACREQPYKGMVFFDQ
>Mature_139_residues
MLSGLNHLTLAVSQLAPSVAFYQQLLGMTLHARWDSGAYLSCGDLWLCLSLDPQRRVTPPEESDYTHYAFSISEADFASF
AARLEAAGVAIWKLNRSEGASHYFLDPDGHKLELHVGSLAQRLAACREQPYKGMVFFDQ

Specific function: Metalloglutathione transferase which confers resistance to fosfomycin by catalyzing the addition of glutathione to fosfomycin [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =2.5.1.18 [H]

Molecular weight: Translated: 15465; Mature: 15465

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGLNHLTLAVSQLAPSVAFYQQLLGMTLHARWDSGAYLSCGDLWLCLSLDPQRRVTPP
CCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCEEEECCEEEEEECCCCCCCCCC
EESDYTHYAFSISEADFASFAARLEAAGVAIWKLNRSEGASHYFLDPDGHKLELHVGSLA
CCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCEEEEEHHHHH
QRLAACREQPYKGMVFFDQ
HHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MLSGLNHLTLAVSQLAPSVAFYQQLLGMTLHARWDSGAYLSCGDLWLCLSLDPQRRVTPP
CCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCEEEECCEEEEEECCCCCCCCCC
EESDYTHYAFSISEADFASFAARLEAAGVAIWKLNRSEGASHYFLDPDGHKLELHVGSLA
CCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCEEEEEHHHHH
QRLAACREQPYKGMVFFDQ
HHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1963292 [H]