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The map label for this gene is celF [H]

Identifier: 238892733

GI number: 238892733

Start: 517921

End: 519228

Strand: Direct

Name: celF [H]

Synonym: KP1_0528

Alternate gene names: 238892733

Gene position: 517921-519228 (Clockwise)

Preceding gene: 238892732

Following gene: 238892734

Centisome position: 9.87

GC content: 60.93

Gene sequence:

>1308_bases
ATGCAAGCACTGAAAATTGCCGTTATCGGCGGCGGCAGCAGCTACACGCCGGAGCTTATTGAAGGGATTATCGTTCGTTA
CGAGCAGCTTCCGGTCACAGAGCTGGCGCTGGTAGATGTCGAATCCGGCCGGGAAAAGGTGGAGATCATCGCCGCGCTGA
CCCGGCGGATGCTGAAACATAAAGGGCTGGAGCAGGTGGCGGTCAGCGTCCACTTTACGCTCGATGAGGCCATCCGCGGC
GCCAGCTTTGTGCTGACCCAGCTGCGTGTCGGACAGCTGGCGGCGCGCGCCGCCGATGAGCGGCTGGGTCTCAAGTACCA
CCTGCTGGGCCAGGAGACCACCGGCGTCGGCGGTTTTGCCAAGGCGCTGCGGACGATCCCGGTGATCCTTGAGGTGGCGC
GCAAAGTGGAACAGCTGGCGCCGGAAGCGTTCATTCTCAACTTTACTAATCCGGCGGGGATCGTCACCGAGGCGGTCAGC
CGCTACAGCACGGCGAAGATCATCGGCCTGTGCAACGTGCCGATCAACATGCAGCACATGATCGTCGGGATGCTGGGCGC
CCAGGAGTCCGAAGTAAAACTGCGCTTTGCCGGGCTCAACCATATGGTCTGGGTGCATAAGGTGCTACAGGGGCGCGAGG
ATGTCACCGGCAAAGTGATCGACATGCTGTGCGACGGTAAGGCGCTGTCGATGAACAACATTAAAGAGCTGCCGTGGCCG
GCGGAATTCCTGCGCGCGCTGAAGGCGATTCCGTGCCCCTATCATCGCTATTTTTGGCTGACGCCGGCGATGCTGGCGGA
AGAGATCGCCGCGGCGAAAACCAAGGGCACCCGCGCCGAACAGGTGATGAAAGTCGAGCAGGAGCTGTTTGCGCTGTACG
CCGACCCGCAGCTGGAAGAAAAACCGGAGCAACTGAGCTTTCGCGGCGGCGCCTACTACTCGGAGGTGGCCGTCGAGCTT
ATCAACGCGATCTACAACAACCTGGGCGCGGAGATGGTGGTGAATACCCGCAACAACGGCGCGATTCATGGCCTGGATGA
TGATGCGGTGGTGGAAACCAACAGCATTATTGATGCGCAAGGGGCGCGGCCGCTGGCGTTCGGCCCGCTGCCGCCGGCGA
TGAATGGCCTGACCCAGCAGGTGAAAGCCTTCGAGCGGTTGACGATTGAAGCCGCGGTACACGGCTGTCGCGAAAGCGCA
CTGCTGGCGCTGGTGACCAACCCGCTGGTCGGTAACGTCACCGATGCCCAGGCGCTGCTGGATGAAGTATTAACCATCAA
CCGTCAATGGCTGACGCAATTTAACTGA

Upstream 100 bases:

>100_bases
CGAACACTAATTTTCTCATCCCGTTTTTCATCATCATCACGTGCGGTCCTGCCGATCGCGCGCGGCGCTCTGTATGCCGA
AAATCCGAAAAGAGGACACT

Downstream 100 bases:

>100_bases
CCGCTATTTCCCCTCGGGAGCACACCATGTCGAATACAAGCTGGATTGAACGTTACGTCATGCCGGCGGCGCTGAGAATC
GCCGGGCAAAAGCACGTCCT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQALKIAVIGGGSSYTPELIEGIIVRYEQLPVTELALVDVESGREKVEIIAALTRRMLKHKGLEQVAVSVHFTLDEAIRG
ASFVLTQLRVGQLAARAADERLGLKYHLLGQETTGVGGFAKALRTIPVILEVARKVEQLAPEAFILNFTNPAGIVTEAVS
RYSTAKIIGLCNVPINMQHMIVGMLGAQESEVKLRFAGLNHMVWVHKVLQGREDVTGKVIDMLCDGKALSMNNIKELPWP
AEFLRALKAIPCPYHRYFWLTPAMLAEEIAAAKTKGTRAEQVMKVEQELFALYADPQLEEKPEQLSFRGGAYYSEVAVEL
INAIYNNLGAEMVVNTRNNGAIHGLDDDAVVETNSIIDAQGARPLAFGPLPPAMNGLTQQVKAFERLTIEAAVHGCRESA
LLALVTNPLVGNVTDAQALLDEVLTINRQWLTQFN

Sequences:

>Translated_435_residues
MQALKIAVIGGGSSYTPELIEGIIVRYEQLPVTELALVDVESGREKVEIIAALTRRMLKHKGLEQVAVSVHFTLDEAIRG
ASFVLTQLRVGQLAARAADERLGLKYHLLGQETTGVGGFAKALRTIPVILEVARKVEQLAPEAFILNFTNPAGIVTEAVS
RYSTAKIIGLCNVPINMQHMIVGMLGAQESEVKLRFAGLNHMVWVHKVLQGREDVTGKVIDMLCDGKALSMNNIKELPWP
AEFLRALKAIPCPYHRYFWLTPAMLAEEIAAAKTKGTRAEQVMKVEQELFALYADPQLEEKPEQLSFRGGAYYSEVAVEL
INAIYNNLGAEMVVNTRNNGAIHGLDDDAVVETNSIIDAQGARPLAFGPLPPAMNGLTQQVKAFERLTIEAAVHGCRESA
LLALVTNPLVGNVTDAQALLDEVLTINRQWLTQFN
>Mature_435_residues
MQALKIAVIGGGSSYTPELIEGIIVRYEQLPVTELALVDVESGREKVEIIAALTRRMLKHKGLEQVAVSVHFTLDEAIRG
ASFVLTQLRVGQLAARAADERLGLKYHLLGQETTGVGGFAKALRTIPVILEVARKVEQLAPEAFILNFTNPAGIVTEAVS
RYSTAKIIGLCNVPINMQHMIVGMLGAQESEVKLRFAGLNHMVWVHKVLQGREDVTGKVIDMLCDGKALSMNNIKELPWP
AEFLRALKAIPCPYHRYFWLTPAMLAEEIAAAKTKGTRAEQVMKVEQELFALYADPQLEEKPEQLSFRGGAYYSEVAVEL
INAIYNNLGAEMVVNTRNNGAIHGLDDDAVVETNSIIDAQGARPLAFGPLPPAMNGLTQQVKAFERLTIEAAVHGCRESA
LLALVTNPLVGNVTDAQALLDEVLTINRQWLTQFN

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=440, Percent_Identity=44.7727272727273, Blast_Score=350, Evalue=8e-98,
Organism=Escherichia coli, GI1790560, Length=455, Percent_Identity=24.3956043956044, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 47625; Mature: 47625

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALKIAVIGGGSSYTPELIEGIIVRYEQLPVTELALVDVESGREKVEIIAALTRRMLKH
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
KGLEQVAVSVHFTLDEAIRGASFVLTQLRVGQLAARAADERLGLKYHLLGQETTGVGGFA
CCHHHEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KALRTIPVILEVARKVEQLAPEAFILNFTNPAGIVTEAVSRYSTAKIIGLCNVPINMQHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHH
IVGMLGAQESEVKLRFAGLNHMVWVHKVLQGREDVTGKVIDMLCDGKALSMNNIKELPWP
HHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCH
AEFLRALKAIPCPYHRYFWLTPAMLAEEIAAAKTKGTRAEQVMKVEQELFALYADPQLEE
HHHHHHHHCCCCCCHHHHEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGAYYSEVAVELINAIYNNLGAEMVVNTRNNGAIHGLDDDAVVETNSIIDAQ
CCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCEEECCCEEECC
GARPLAFGPLPPAMNGLTQQVKAFERLTIEAAVHGCRESALLALVTNPLVGNVTDAQALL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHH
DEVLTINRQWLTQFN
HHHHHHCHHHHHCCC
>Mature Secondary Structure
MQALKIAVIGGGSSYTPELIEGIIVRYEQLPVTELALVDVESGREKVEIIAALTRRMLKH
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
KGLEQVAVSVHFTLDEAIRGASFVLTQLRVGQLAARAADERLGLKYHLLGQETTGVGGFA
CCHHHEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KALRTIPVILEVARKVEQLAPEAFILNFTNPAGIVTEAVSRYSTAKIIGLCNVPINMQHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHH
IVGMLGAQESEVKLRFAGLNHMVWVHKVLQGREDVTGKVIDMLCDGKALSMNNIKELPWP
HHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCH
AEFLRALKAIPCPYHRYFWLTPAMLAEEIAAAKTKGTRAEQVMKVEQELFALYADPQLEE
HHHHHHHHCCCCCCHHHHEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGAYYSEVAVELINAIYNNLGAEMVVNTRNNGAIHGLDDDAVVETNSIIDAQ
CCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCEEECCCEEECC
GARPLAFGPLPPAMNGLTQQVKAFERLTIEAAVHGCRESALLALVTNPLVGNVTDAQALL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHH
DEVLTINRQWLTQFN
HHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]