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The map label for this gene is mpl [H]

Identifier: 238892710

GI number: 238892710

Start: 496857

End: 498230

Strand: Direct

Name: mpl [H]

Synonym: KP1_0501

Alternate gene names: 238892710

Gene position: 496857-498230 (Clockwise)

Preceding gene: 238892707

Following gene: 238892711

Centisome position: 9.47

GC content: 61.28

Gene sequence:

>1374_bases
ATGCGCATTCATATTTTAGGAATCTGTGGCACGTTTATGGGGGGGCTGGCCATGCTGGCGCGCTCCCTCGGTCATGAGGT
AACGGGGTCGGATGCTAACGTCTATCCGCCGATGAGCACCCTGCTGGAAAACCAGGGGATTGATTTAATTCAGGGCTATG
ACCCCAGCCAGCTGGAGCCCCGGCCGGACCTGGTGATCATCGGTAACGCCATGACTCGCGGCAACCCGTGCGTTGAAGCG
GTGCTGGAAAATAATATTCCCTATATGTCCGGCCCGCAGTGGCTGCACGACTTCGTGCTGCGCGATCGCTGGGTGCTGGC
CGTCGCCGGGACCCACGGCAAAACCACCACTGCCGGCATGGCGACCTGGATCCTTGATGCCTGCGGCTATAAACCGGGGT
TCGTGATTGGCGGCGTTCCGGGCAATTTCGACGTCTCCGCGCGCCTGGGCGACAGCCCGTTCTTCGTCATTGAAGCCGAC
GAATATGACTGCGCCTTCTTCGACAAGCGCTCCAAGTTTGTTCACTACTGCCCGCGGACGCTGATCCTCAACAACCTTGA
GTTCGACCACGCGGACATCTTTGATGACCTGAAGGCGATTCAGAAACAATTCCACCATCTGGTGCGCATCGTGCCGGGCA
AAGGCAAAATTATCCTGCCGGAGAACGACATCAACCTGAAGCAGGTGATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTG
GTGGGCGAGCAGGGCCACTGGCAGGCGAAAAAACTGAACGCCGACGCCTCGCAGTGGGAAGTCTTACTCGACGGCGAAAA
GGTCGGCGAAGTGAAGTGGGCGCTGGTCGGCGAGCATAATATGCATAACGGCCTGATGGCGATTGCCGCGGCGCGTCACG
TCGGCGTGCTGCCGGCGGATGCCGCGAACGCGCTGGGCAGCTTTATCAACGCCCGCCGTCGCCTGGAGCTGCGCGGCGAA
GCCAACGGCGTCACCGTGTATGATGATTTCGCCCATCATCCAACGGCAATCCTCGCCACGCTGCAGGCGCTGCGCGGTAA
AGTAGGCGGTACCGCGCGCATTCTCGCCGTGCTGGAGCCGCGCTCTAACACCATGAAAATGGGCGTCTGCAAGGACGATC
TGGCGCCGTCTCTCGGCCGCGCCGATGAAGTGTTCCTCCTGCAGCCGCAGCATATTCCGTGGCAGGTGGCGGAAGTGGCG
GAAGCCTGCGTCCAGCCGGCCCACTGGAGCGCCGACGTCGATACCCTGGCGGAGATGGTGGTCAAAACCGCCCACCCGGG
CGACCATATCCTGGTGATGAGCAACGGCGGCTTCGGCGGCATTCATCAGAAGCTGCTGGATAAGCTGGCGCAAAAAGCGG
CCGCGGCTGAATAA

Upstream 100 bases:

>100_bases
GGTGAATCGCGCCAGCAAAATACAGATTCGCCTGAAATGCATTTCGCCAGGGCCCGGGATATGGGTAAAATGTGCCCCGG
ATAAGAAAAGGAAATGACGA

Downstream 100 bases:

>100_bases
GCGCGTACGCCTCCGGTCACTGCCCTCCGCGTGAGGGCAGTTTTGTTTTAGCTACCAGGAATTGTGTGATGCTGACCTGC
GAAAGCGTACGTTCTACCGA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso- diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLENQGIDLIQGYDPSQLEPRPDLVIIGNAMTRGNPCVEA
VLENNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILDACGYKPGFVIGGVPGNFDVSARLGDSPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGKGKIILPENDINLKQVMAMGCWSEQEL
VGEQGHWQAKKLNADASQWEVLLDGEKVGEVKWALVGEHNMHNGLMAIAAARHVGVLPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLQALRGKVGGTARILAVLEPRSNTMKMGVCKDDLAPSLGRADEVFLLQPQHIPWQVAEVA
EACVQPAHWSADVDTLAEMVVKTAHPGDHILVMSNGGFGGIHQKLLDKLAQKAAAAE

Sequences:

>Translated_457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLENQGIDLIQGYDPSQLEPRPDLVIIGNAMTRGNPCVEA
VLENNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILDACGYKPGFVIGGVPGNFDVSARLGDSPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGKGKIILPENDINLKQVMAMGCWSEQEL
VGEQGHWQAKKLNADASQWEVLLDGEKVGEVKWALVGEHNMHNGLMAIAAARHVGVLPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLQALRGKVGGTARILAVLEPRSNTMKMGVCKDDLAPSLGRADEVFLLQPQHIPWQVAEVA
EACVQPAHWSADVDTLAEMVVKTAHPGDHILVMSNGGFGGIHQKLLDKLAQKAAAAE
>Mature_457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLENQGIDLIQGYDPSQLEPRPDLVIIGNAMTRGNPCVEA
VLENNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILDACGYKPGFVIGGVPGNFDVSARLGDSPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGKGKIILPENDINLKQVMAMGCWSEQEL
VGEQGHWQAKKLNADASQWEVLLDGEKVGEVKWALVGEHNMHNGLMAIAAARHVGVLPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLQALRGKVGGTARILAVLEPRSNTMKMGVCKDDLAPSLGRADEVFLLQPQHIPWQVAEVA
EACVQPAHWSADVDTLAEMVVKTAHPGDHILVMSNGGFGGIHQKLLDKLAQKAAAAE

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=457, Percent_Identity=92.9978118161926, Blast_Score=886, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=459, Percent_Identity=27.4509803921569, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49804; Mature: 49804

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLENQGIDLIQGYDPSQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCC
RPDLVIIGNAMTRGNPCVEAVLENNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILDACGYKPGFVIGGVPGNFDVSARLGDSPFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGKGKIILPENDINLKQVMAMGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHCCCCHHHH
VGEQGHWQAKKLNADASQWEVLLDGEKVGEVKWALVGEHNMHNGLMAIAAARHVGVLPAD
CCCCCCCCHHHCCCCHHHEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCH
AANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLQALRGKVGGTARILAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGVCKDDLAPSLGRADEVFLLQPQHIPWQVAEVAEACVQPAHWSADVDTLAEMV
CCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAHPGDHILVMSNGGFGGIHQKLLDKLAQKAAAAE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLENQGIDLIQGYDPSQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCC
RPDLVIIGNAMTRGNPCVEAVLENNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILDACGYKPGFVIGGVPGNFDVSARLGDSPFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGKGKIILPENDINLKQVMAMGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHCCCCHHHH
VGEQGHWQAKKLNADASQWEVLLDGEKVGEVKWALVGEHNMHNGLMAIAAARHVGVLPAD
CCCCCCCCHHHCCCCHHHEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCH
AANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLQALRGKVGGTARILAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGVCKDDLAPSLGRADEVFLLQPQHIPWQVAEVAEACVQPAHWSADVDTLAEMV
CCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAHPGDHILVMSNGGFGGIHQKLLDKLAQKAAAAE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]