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The map label for this gene is cpdB [H]

Identifier: 238892692

GI number: 238892692

Start: 477287

End: 479230

Strand: Reverse

Name: cpdB [H]

Synonym: KP1_0483

Alternate gene names: 238892692

Gene position: 479230-477287 (Counterclockwise)

Preceding gene: 238892694

Following gene: 238892690

Centisome position: 9.13

GC content: 57.66

Gene sequence:

>1944_bases
ATGATTAAGTTTAGCGCAACGCTCCTGGCCACGCTGATCGCCGCCAGCGTCAACGCCGCGACGGTCGACCTGCGGATCAT
GGAAACCACCGATCTGCACAGCAATATGATGGACTTCGACTATTACAAAGATGCCGCCACGGAGAAATTCGGCCTGGTGC
GTACCGCCACCCTGATCGAACAGGCGCGGGCAGAAGTTAAAAACAGCGTGCTGGTGGACAACGGGGATGTGATCCAGGGG
AGCCCGTTAGGCGACTATATGGCGGCAAAAGGGCTCAAAGAGGGCGATGTACATCCGGTATATAAGGCGATGAACACCCT
GAATTACGCCGTCGGCAACCTGGGCAACCATGAGTTCAATTACGGTCTCGACTTCCTGCACAAGGCGCTGGCCGGGGCGA
AGTTCCCCTACGTGAACGCCAATATCATCGATGCCAAAACCGGCAAACCGATGTTTACGCCGTACCTGATCCAGGATACC
CGGGTCGTGGACAGCGACGGTCAAATCCACACCCTGCGCATCGGCTATATCGGCTTTGTGCCGCCGCAGATCATGACCTG
GGATAAAGCCAATCTTAACGGCAAGGTGACGGTCAATGACATTACCGAAACCGCGCGCAAATATATCCCGGAGATGCGGG
CGAAAGGGGCTGATGTGGTGGTAGTGGTCGCTCACTCCGGCCTCTCCGCCGACCCGTATCAGGCGATGGCCGAGAATTCG
GTTTACTACTTAAGCCAGGTGCCCGGCGTGGACGCCATTATGTTCGGCCATGCCCACGCGGTGTTCCCGGGTAAAGACTT
CGCCAACATCAAAGGCGCGGATATCGCCAAAGGGACGCTAAACGGCGTACCGGCGGTGATGCCGGGAATGTGGGGCGATC
ATCTCGGGGTGGTCGATCTGGTGCTAAATAACGACAGCGGTAAATGGCAGGTAACTCAGAGTAAAGCGGAAGCGCGGCCA
ATCTATGACGCGGTGGCGAAGAAATCACTGGCGGCCGAAGATGGCAAGCTGGTGGCGGTGCTGAAAGCCGATCATGACGC
CACTCGCGAATTCGTCAGCAAGCCGATCGGCAAGTCCGCCGACAACATGTACAGCTATCTGGCGCTGGTGCAGGACGACC
CGACGGTGCAGGTGGTCAACATGGCGCAGAAAGCCTACGTAGAACACTATATTCAGGGCGACCCGGATCTGGCGAAGCTG
CCGGTGCTCTCCGCCGCCGCGCCGTTTAAAGTCGGCGGGCGTAAAAACGATCCGGCGAGCTTTGTCGAAGTGGAGAAAGG
CCAGCTCACCTTCCGCAATGCCGCCGACCTCTATCTCTACCCCAACACCCTGGTGGTGATGAAGGTCAGCGGGAAAGAGG
TTAAGGAGTGGCTGGAATGCTCTGCCGGACAGTTTAACCAGATCGATCCCGCCAGCAGCAAGCCGCAGTCGCTGATCAAC
TGGGACGGCTTCCGCACCTACAACTTCGACGTCATTGACGGGGTGAACTACCAGATCGACGTCACTCAGCCGGCCCGCTA
CGACGGCGAATGTCAGATGATCCATCCGCAGGCAGAGCGTATTAAGCACCTGACCTTCAATGGAAAACCGGTTGATCCGC
AGGCTACTTTCCTGGTCGCGACCAATAACTATCGCGCTTACGGCGGCAAATTCGCCGGCACCGGTGAGAGCCATATCGCC
TTCGCCTCACCGGATGAAAACCGTTCGGTGCTGGCGGCGTGGATTGGCGCGCAATCGAAGAAGGAAGGCGCGATCCATCC
GGCGGCGGATAACAACTGGCGTCTGGCGCCGATCCACAGCAACACGCCGTTGGATATTCGTTTTGAGACCTCGCCTGGCG
ACAAAGCCGCGGCGTTTATCAAAGAGAAAGCGCAGTATCCAATGCGCCAGGTGGCCACCGATGATATCGGCTTCGCCATT
TATCAGCTGGATTTGAGCAAGTAA

Upstream 100 bases:

>100_bases
TTTCTCTGCCCGCTTTTCTGGCAAGATTCACATTTCTGTAAGCAACAGAATATAAATACATTTTCTTCTGTGCTATCGCT
CATAAAAAAGGACGCCTCTG

Downstream 100 bases:

>100_bases
TGCCTGTTCCCCGGTGGCGCTGCGCTTACCGGGGCTACAGGGAGTGATACATTTTCACTCCCCTTGGTTAGCCAGCACCG
AGGGCAGATTAACCTCGATC

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDAATEKFGLVRTATLIEQARAEVKNSVLVDNGDVIQG
SPLGDYMAAKGLKEGDVHPVYKAMNTLNYAVGNLGNHEFNYGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDT
RVVDSDGQIHTLRIGYIGFVPPQIMTWDKANLNGKVTVNDITETARKYIPEMRAKGADVVVVVAHSGLSADPYQAMAENS
VYYLSQVPGVDAIMFGHAHAVFPGKDFANIKGADIAKGTLNGVPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQSKAEARP
IYDAVAKKSLAAEDGKLVAVLKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMIHPQAERIKHLTFNGKPVDPQATFLVATNNYRAYGGKFAGTGESHIA
FASPDENRSVLAAWIGAQSKKEGAIHPAADNNWRLAPIHSNTPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAI
YQLDLSK

Sequences:

>Translated_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDAATEKFGLVRTATLIEQARAEVKNSVLVDNGDVIQG
SPLGDYMAAKGLKEGDVHPVYKAMNTLNYAVGNLGNHEFNYGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDT
RVVDSDGQIHTLRIGYIGFVPPQIMTWDKANLNGKVTVNDITETARKYIPEMRAKGADVVVVVAHSGLSADPYQAMAENS
VYYLSQVPGVDAIMFGHAHAVFPGKDFANIKGADIAKGTLNGVPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQSKAEARP
IYDAVAKKSLAAEDGKLVAVLKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMIHPQAERIKHLTFNGKPVDPQATFLVATNNYRAYGGKFAGTGESHIA
FASPDENRSVLAAWIGAQSKKEGAIHPAADNNWRLAPIHSNTPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAI
YQLDLSK
>Mature_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDAATEKFGLVRTATLIEQARAEVKNSVLVDNGDVIQG
SPLGDYMAAKGLKEGDVHPVYKAMNTLNYAVGNLGNHEFNYGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDT
RVVDSDGQIHTLRIGYIGFVPPQIMTWDKANLNGKVTVNDITETARKYIPEMRAKGADVVVVVAHSGLSADPYQAMAENS
VYYLSQVPGVDAIMFGHAHAVFPGKDFANIKGADIAKGTLNGVPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQSKAEARP
IYDAVAKKSLAAEDGKLVAVLKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMIHPQAERIKHLTFNGKPVDPQATFLVATNNYRAYGGKFAGTGESHIA
FASPDENRSVLAAWIGAQSKKEGAIHPAADNNWRLAPIHSNTPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAI
YQLDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=647, Percent_Identity=87.1715610510046, Blast_Score=1182, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=577, Percent_Identity=24.7833622183709, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921980, Length=178, Percent_Identity=24.7191011235955, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652512, Length=179, Percent_Identity=24.5810055865922, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161076508, Length=178, Percent_Identity=24.7191011235955, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 70639; Mature: 70639

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDAATEKFGLVRTATLIE
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QARAEVKNSVLVDNGDVIQGSPLGDYMAAKGLKEGDVHPVYKAMNTLNYAVGNLGNHEFN
HHHHHHHCCEEECCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDTRVVDSDGQIHTLRIGYIGFV
CCHHHHHHHHHCCCCCEECCEEEECCCCCCCCCEEEEECCEEECCCCCEEEEEEEEEECC
PPQIMTWDKANLNGKVTVNDITETARKYIPEMRAKGADVVVVVAHSGLSADPYQAMAENS
CCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCC
VYYLSQVPGVDAIMFGHAHAVFPGKDFANIKGADIAKGTLNGVPAVMPGMWGDHLGVVDL
EEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEE
VLNNDSGKWQVTQSKAEARPIYDAVAKKSLAAEDGKLVAVLKADHDATREFVSKPIGKSA
EEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
FVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCHHCC
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMIHPQAERIKHLTFNGKPVDPQATFLVA
CCCEEEEEEEEECCCCEEEEECCCCCCCCCEEEECCHHHHEEEEEECCCCCCCCEEEEEE
TNNYRAYGGKFAGTGESHIAFASPDENRSVLAAWIGAQSKKEGAIHPAADNNWRLAPIHS
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
NTPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAIYQLDLSK
CCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDAATEKFGLVRTATLIE
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QARAEVKNSVLVDNGDVIQGSPLGDYMAAKGLKEGDVHPVYKAMNTLNYAVGNLGNHEFN
HHHHHHHCCEEECCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDTRVVDSDGQIHTLRIGYIGFV
CCHHHHHHHHHCCCCCEECCEEEECCCCCCCCCEEEEECCEEECCCCCEEEEEEEEEECC
PPQIMTWDKANLNGKVTVNDITETARKYIPEMRAKGADVVVVVAHSGLSADPYQAMAENS
CCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCC
VYYLSQVPGVDAIMFGHAHAVFPGKDFANIKGADIAKGTLNGVPAVMPGMWGDHLGVVDL
EEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEE
VLNNDSGKWQVTQSKAEARPIYDAVAKKSLAAEDGKLVAVLKADHDATREFVSKPIGKSA
EEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
FVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCHHCC
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMIHPQAERIKHLTFNGKPVDPQATFLVA
CCCEEEEEEEEECCCCEEEEECCCCCCCCCEEEECCHHHHEEEEEECCCCCCCCEEEEEE
TNNYRAYGGKFAGTGESHIAFASPDENRSVLAAWIGAQSKKEGAIHPAADNNWRLAPIHS
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
NTPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAIYQLDLSK
CCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]