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The map label for this gene is ptxA [H]

Identifier: 238892674

GI number: 238892674

Start: 463614

End: 464081

Strand: Direct

Name: ptxA [H]

Synonym: KP1_0462

Alternate gene names: 238892674

Gene position: 463614-464081 (Clockwise)

Preceding gene: 238892673

Following gene: 238892675

Centisome position: 8.83

GC content: 57.91

Gene sequence:

>468_bases
ATGAAACTCCATGAATCACTGGCGCAGAATCACTCCATTCGCCTGCAGGCGGAAGCGGAAACCTGGCAGGAGGCGGTGAA
AATCGGCGTCGATCTGCTGGTGGCCGCCGACGTGGTGGAACCGCGCTACTACCAGGCAATCCTCGACGGCGTCGCGCAGC
ACGGCCCCTATTTTGTGATCGCTCCGGGATTAGCCATGCCGCACGGGCGGCCGGAAGAGGGCGTCAAAAAAACCGGCTTT
GCGCTGGTCACGCTAAAGAAACCGCTGATTTTCAACCACGAAGATAACGACCCGGTCGACATCCTGATCACCATGGCGGC
GGTTGACGCCACCACCCACCAGGAAGTGGGCATCATGCAGATCGTTAACCTGTTTGATGACGAAGCCAATTTCGACCGTT
TACGCGCCTGCCGCAGTGAGCAGGAAGTGCTGGATTTAATCAAAAACGCCACCGCGGCGGCTGTTTAA

Upstream 100 bases:

>100_bases
GGTGGGGGTGCGTAATATGCTTTCCCCCGCGGATTTCGGGCCAAAGCTGCTGGAGGTGATTAAAGCTCATTTCCCGCAGG
ACGTGAAATAAGGAGCGGCT

Downstream 100 bases:

>100_bases
AAGGAACACTATCATGTCATTACCTATGTTACAGGTCGCCCTCGACAACCAAACGCTGGCCAGCGCTTATGAAACAACCC
GTCTGATCGCCGAAGAGGTA

Product: PTS system L-ascorbate-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKLHESLAQNHSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVAQHGPYFVIAPGLAMPHGRPEEGVKKTGF
ALVTLKKPLIFNHEDNDPVDILITMAAVDATTHQEVGIMQIVNLFDDEANFDRLRACRSEQEVLDLIKNATAAAV

Sequences:

>Translated_155_residues
MKLHESLAQNHSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVAQHGPYFVIAPGLAMPHGRPEEGVKKTGF
ALVTLKKPLIFNHEDNDPVDILITMAAVDATTHQEVGIMQIVNLFDDEANFDRLRACRSEQEVLDLIKNATAAAV
>Mature_155_residues
MKLHESLAQNHSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVAQHGPYFVIAPGLAMPHGRPEEGVKKTGF
ALVTLKKPLIFNHEDNDPVDILITMAAVDATTHQEVGIMQIVNLFDDEANFDRLRACRSEQEVLDLIKNATAAAV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=154, Percent_Identity=88.961038961039, Blast_Score=283, Evalue=3e-78,
Organism=Escherichia coli, GI1790755, Length=116, Percent_Identity=36.2068965517241, Blast_Score=92, Evalue=1e-20,
Organism=Escherichia coli, GI1789302, Length=120, Percent_Identity=37.5, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17083; Mature: 17083

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHESLAQNHSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVAQHGPYFVI
CCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
APGLAMPHGRPEEGVKKTGFALVTLKKPLIFNHEDNDPVDILITMAAVDATTHQEVGIMQ
ECCCCCCCCCCCCCHHHCCEEEEEECCCEEECCCCCCCCEEEEEEHHHCCCCCHHHHHHH
IVNLFDDEANFDRLRACRSEQEVLDLIKNATAAAV
HHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLHESLAQNHSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVAQHGPYFVI
CCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
APGLAMPHGRPEEGVKKTGFALVTLKKPLIFNHEDNDPVDILITMAAVDATTHQEVGIMQ
ECCCCCCCCCCCCCHHHCCEEEEEECCCEEECCCCCCCCEEEEEEHHHCCCCCHHHHHHH
IVNLFDDEANFDRLRACRSEQEVLDLIKNATAAAV
HHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]