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The map label for this gene is amiB [H]

Identifier: 238892653

GI number: 238892653

Start: 441887

End: 443242

Strand: Direct

Name: amiB [H]

Synonym: KP1_0437

Alternate gene names: 238892653

Gene position: 441887-443242 (Clockwise)

Preceding gene: 238892652

Following gene: 238892654

Centisome position: 8.42

GC content: 61.73

Gene sequence:

>1356_bases
ATGAAGTATCGCATAACCACCTGGCTTGCCGCTGCGCTGATGCTGTCGAGTCTTCACGCCACTGCGGCGAGTTTATCCGA
TATCCAGGTCTCTAACGGAGACCAGCAGGCCCGTATTACCCTAAGCTTTGTCGGTGAGCCGGAGTACTCCTTTACCCCAC
AGGGTAAACGTCTGGTGGCGCTGGATATCAAGCAGACCGGCGTATTGCAGGGACTACCGCTGCAGTTTAGCGGCAGCAAC
CTGGTGAAGAGTATTCGCGCCGGCACGCCGCAGGATACGCAGACGCTGCGTCTGCTGGTGGATCTGACGGAAGATGGCAA
AACCCGGGCGGTGAAGCAACAGAACGGGGCGAATTACACGGTGGTCTTTACCATCAACGCCGACGCGCCGCCGCCGCCGC
CTCCGCCGCCCGTGGTGGTGAAACGGGCCGACCCGCCGCCGGTGATGCCTTCCCGGACGACGACGTCAGGGCGTAACCCG
TTCAGCTCGTCGGGTCATGAACGGCAGACGGTGATGACCTCCTCTCAGCCGGCGATGACCAACAATCAGACCGTGACTCG
CCCGGCGGCCCGTACGGTGTCGGCGGATGATAAGGTGATTATCGCCATCGATGCCGGACACGGCGGTCAGGATCCTGGCG
CGATTGGCCCGAACGGCACCAAAGAGAAAAACGTGACTATCGCTATCGCGCGCAAGCTGCGGGCGTTACTGAACGCCGAT
CCGCAGTTTAAGCCGGTGCTGACCCGCGACGGGGATTACTTTATTTCGGTGATGGGCCGCTCTGACGTGGCGCGTAAGCA
AAACGCCAATTTCCTCGTCTCTATCCATGCTGACGCCGCGCCAAACCGCGATGCCACTGGCGCCTCGGTGTGGGTGCTCT
CGAACCGTCGCGCCAACAGCGAAATGGCCGGTTGGCTCGAGCAGCATGAGAAACAGTCTGAACTGCTCGGCGGTGCGGGC
GATGTGCTGGCAAACAGCCAGGCCGACCCCTACCTGAGCCAGGCGGTGCTGGATCTGCAGTTTGGTCATTCCCAGCGCGT
CGGCTACGATGTGGCGACCAACGTGCTGAGCCAGCTGCAGCGTATTGGCAACCTGCACAAACGCCGCCCGGAGCATGCCA
GCCTTGGAGTGCTGCGCTCGCCGGACATCCCGTCGATCCTCGTGGAAACGGGCTTTATCAGTAACAACGGCGAAGAGCGG
CTGCTGGGGAGCGATGATTACCAGGAGCAGATTGCGGAGGCGATTTATAACGGCTTACGCAACTACTTTATGCAGCATCC
GCTGCAGTCGGCGCCTCGCGGCGAGGCGGCGCAGACCGCCAGCGCCGCGGCGCCGGGCGGGATGTTGATTAACTAA

Upstream 100 bases:

>100_bases
GATTACCAGGCACAAGGGCGTGAGGCGCGTATTACCGCGATCTCCTCACGGGGTGAATCTTTACTGGCGCGTTTAGCCTT
TTAGCCTGAAGGATGACGGG

Downstream 100 bases:

>100_bases
GGAGCAGGGGATGCCGATTCAGGTTCTGCCGCCGCAGCTCGCCAACCAGATTGCCGCCGGCGAGGTGGTGGAGCGGCCCG
CGTCGGTGGTGAAAGAGCTG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKYRITTWLAAALMLSSLHATAASLSDIQVSNGDQQARITLSFVGEPEYSFTPQGKRLVALDIKQTGVLQGLPLQFSGSN
LVKSIRAGTPQDTQTLRLLVDLTEDGKTRAVKQQNGANYTVVFTINADAPPPPPPPPVVVKRADPPPVMPSRTTTSGRNP
FSSSGHERQTVMTSSQPAMTNNQTVTRPAARTVSADDKVIIAIDAGHGGQDPGAIGPNGTKEKNVTIAIARKLRALLNAD
PQFKPVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMAGWLEQHEKQSELLGGAG
DVLANSQADPYLSQAVLDLQFGHSQRVGYDVATNVLSQLQRIGNLHKRRPEHASLGVLRSPDIPSILVETGFISNNGEER
LLGSDDYQEQIAEAIYNGLRNYFMQHPLQSAPRGEAAQTASAAAPGGMLIN

Sequences:

>Translated_451_residues
MKYRITTWLAAALMLSSLHATAASLSDIQVSNGDQQARITLSFVGEPEYSFTPQGKRLVALDIKQTGVLQGLPLQFSGSN
LVKSIRAGTPQDTQTLRLLVDLTEDGKTRAVKQQNGANYTVVFTINADAPPPPPPPPVVVKRADPPPVMPSRTTTSGRNP
FSSSGHERQTVMTSSQPAMTNNQTVTRPAARTVSADDKVIIAIDAGHGGQDPGAIGPNGTKEKNVTIAIARKLRALLNAD
PQFKPVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMAGWLEQHEKQSELLGGAG
DVLANSQADPYLSQAVLDLQFGHSQRVGYDVATNVLSQLQRIGNLHKRRPEHASLGVLRSPDIPSILVETGFISNNGEER
LLGSDDYQEQIAEAIYNGLRNYFMQHPLQSAPRGEAAQTASAAAPGGMLIN
>Mature_451_residues
MKYRITTWLAAALMLSSLHATAASLSDIQVSNGDQQARITLSFVGEPEYSFTPQGKRLVALDIKQTGVLQGLPLQFSGSN
LVKSIRAGTPQDTQTLRLLVDLTEDGKTRAVKQQNGANYTVVFTINADAPPPPPPPPVVVKRADPPPVMPSRTTTSGRNP
FSSSGHERQTVMTSSQPAMTNNQTVTRPAARTVSADDKVIIAIDAGHGGQDPGAIGPNGTKEKNVTIAIARKLRALLNAD
PQFKPVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMAGWLEQHEKQSELLGGAG
DVLANSQADPYLSQAVLDLQFGHSQRVGYDVATNVLSQLQRIGNLHKRRPEHASLGVLRSPDIPSILVETGFISNNGEER
LLGSDDYQEQIAEAIYNGLRNYFMQHPLQSAPRGEAAQTASAAAPGGMLIN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=442, Percent_Identity=77.3755656108597, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI87082163, Length=228, Percent_Identity=40.7894736842105, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1788776, Length=238, Percent_Identity=39.4957983193277, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48434; Mature: 48434

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRITTWLAAALMLSSLHATAASLSDIQVSNGDQQARITLSFVGEPEYSFTPQGKRLVA
CCEEHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIKQTGVLQGLPLQFSGSNLVKSIRAGTPQDTQTLRLLVDLTEDGKTRAVKQQNGANYT
EECCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEHHHCCCCCEE
VVFTINADAPPPPPPPPVVVKRADPPPVMPSRTTTSGRNPFSSSGHERQTVMTSSQPAMT
EEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
NNQTVTRPAARTVSADDKVIIAIDAGHGGQDPGAIGPNGTKEKNVTIAIARKLRALLNAD
CCCEEECCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCC
PQFKPVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANS
CCCCEEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCH
EMAGWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGHSQRVGYDVATNVLSQLQ
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RIGNLHKRRPEHASLGVLRSPDIPSILVETGFISNNGEERLLGSDDYQEQIAEAIYNGLR
HHHHHHHCCCCCCCCCEEECCCCCEEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NYFMQHPLQSAPRGEAAQTASAAAPGGMLIN
HHHHHCCHHCCCCCCHHHHHHCCCCCCEECC
>Mature Secondary Structure
MKYRITTWLAAALMLSSLHATAASLSDIQVSNGDQQARITLSFVGEPEYSFTPQGKRLVA
CCEEHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIKQTGVLQGLPLQFSGSNLVKSIRAGTPQDTQTLRLLVDLTEDGKTRAVKQQNGANYT
EECCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEHHHCCCCCEE
VVFTINADAPPPPPPPPVVVKRADPPPVMPSRTTTSGRNPFSSSGHERQTVMTSSQPAMT
EEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
NNQTVTRPAARTVSADDKVIIAIDAGHGGQDPGAIGPNGTKEKNVTIAIARKLRALLNAD
CCCEEECCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCC
PQFKPVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANS
CCCCEEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCH
EMAGWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGHSQRVGYDVATNVLSQLQ
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RIGNLHKRRPEHASLGVLRSPDIPSILVETGFISNNGEERLLGSDDYQEQIAEAIYNGLR
HHHHHHHCCCCCCCCCEEECCCCCEEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NYFMQHPLQSAPRGEAAQTASAAAPGGMLIN
HHHHHCCHHCCCCCCHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]