Click here to switch to the map view.

The map label for this gene is artJ [H]

Identifier: 238892648

GI number: 238892648

Start: 437088

End: 437819

Strand: Reverse

Name: artJ [H]

Synonym: KP1_0432

Alternate gene names: 238892648

Gene position: 437819-437088 (Counterclockwise)

Preceding gene: 238892650

Following gene: 238892646

Centisome position: 8.34

GC content: 58.74

Gene sequence:

>732_bases
ATGAGAAAAGTCCTCACCGTCCTGCTGCTGAGCACGCTGGCCATCGGAACCGCGTCAGCCGAGACGCTCCATTTTGGCAC
CACCACGGCCAATCCACCGTTTGTCACCGCCAACGGCAAAAATCAGCCCGTTGGCTTCGATATCGATCTCGCGCACGCCC
TGTGCCAGCAGATGCAGGCGCAGTGCCAGTTCACGGCGCAGCGTTTCGATACCCTGATCCCGGCTTTACGCTTTAAGAAA
TTTGACGCGGTGATTGCCGGCATGGAAGTGATCCCGATGCGTGAGAAGCAGGTCGCCTTCAGCCGACCCTATCGCCAGGC
GCTCTCCGGGGTAGTGCTCGTCAACAAAGATGTCGCCCATTCCTTTGCCGATCTGAAGGCTAAAAAAGTCGGCGTGGTGA
AAGGCACGCTGCATCAGCGCTACCTGCGTGATAAACAGAAAGCCGTACAGGCTGTCCCTTACGACGATGTCGCCAGCGCC
CTGGCCGCGCTGAAAGCCGGGCAAATAACCGGCGTCATGGGCGACTTCGCCACCCTCGACGCCTGGCAGCAGGAGAACCC
TGACTACGCCATCATGGATGAACGGGCCACCGACCCGGCTTATTACGGTAAGCAGTATGCGATTGCGGTGCGCAAAGACG
ATCCGGAGCTGCTGAACGCCATCAACGATGCCCTGGCCGCGGTCATGGCGACACCTGACTTCCAGCAGATGCAGCAGAAG
TGGTTTAAATAA

Upstream 100 bases:

>100_bases
TCGCTTTTTCCGCCCGGCTGAATGACGCAAAATCGCTCGCATCATACAGCGGTGGCGCGACGTATAACGAGGCCACCCCG
ACGCCAAACAGGGAATTAGC

Downstream 100 bases:

>100_bases
CGCAGAAAAGTGTACCGGGCCGCGCACAGGGCCCGGTCGCGGTTTACAGTTTAATGAAGTGTTCGCGGTAGTAAGCCAGC
TCGGCGACCGACTCGCGGAT

Product: putative arginine-binding periplasmic protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRKVLTVLLLSTLAIGTASAETLHFGTTTANPPFVTANGKNQPVGFDIDLAHALCQQMQAQCQFTAQRFDTLIPALRFKK
FDAVIAGMEVIPMREKQVAFSRPYRQALSGVVLVNKDVAHSFADLKAKKVGVVKGTLHQRYLRDKQKAVQAVPYDDVASA
LAALKAGQITGVMGDFATLDAWQQENPDYAIMDERATDPAYYGKQYAIAVRKDDPELLNAINDALAAVMATPDFQQMQQK
WFK

Sequences:

>Translated_243_residues
MRKVLTVLLLSTLAIGTASAETLHFGTTTANPPFVTANGKNQPVGFDIDLAHALCQQMQAQCQFTAQRFDTLIPALRFKK
FDAVIAGMEVIPMREKQVAFSRPYRQALSGVVLVNKDVAHSFADLKAKKVGVVKGTLHQRYLRDKQKAVQAVPYDDVASA
LAALKAGQITGVMGDFATLDAWQQENPDYAIMDERATDPAYYGKQYAIAVRKDDPELLNAINDALAAVMATPDFQQMQQK
WFK
>Mature_243_residues
MRKVLTVLLLSTLAIGTASAETLHFGTTTANPPFVTANGKNQPVGFDIDLAHALCQQMQAQCQFTAQRFDTLIPALRFKK
FDAVIAGMEVIPMREKQVAFSRPYRQALSGVVLVNKDVAHSFADLKAKKVGVVKGTLHQRYLRDKQKAVQAVPYDDVASA
LAALKAGQITGVMGDFATLDAWQQENPDYAIMDERATDPAYYGKQYAIAVRKDDPELLNAINDALAAVMATPDFQQMQQK
WFK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=242, Percent_Identity=45.4545454545455, Blast_Score=243, Evalue=7e-66,
Organism=Escherichia coli, GI1787088, Length=244, Percent_Identity=40.5737704918033, Blast_Score=204, Evalue=3e-54,
Organism=Escherichia coli, GI1788649, Length=251, Percent_Identity=31.0756972111554, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=29.8245614035088, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788228, Length=226, Percent_Identity=30.0884955752212, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=27.5303643724696, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26780; Mature: 26780

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLTVLLLSTLAIGTASAETLHFGTTTANPPFVTANGKNQPVGFDIDLAHALCQQMQA
CHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
QCQFTAQRFDTLIPALRFKKFDAVIAGMEVIPMREKQVAFSRPYRQALSGVVLVNKDVAH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCEEEECHHHHH
SFADLKAKKVGVVKGTLHQRYLRDKQKAVQAVPYDDVASALAALKAGQITGVMGDFATLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
AWQQENPDYAIMDERATDPAYYGKQYAIAVRKDDPELLNAINDALAAVMATPDFQQMQQK
HHHCCCCCEEEECCCCCCCHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WFK
HCC
>Mature Secondary Structure
MRKVLTVLLLSTLAIGTASAETLHFGTTTANPPFVTANGKNQPVGFDIDLAHALCQQMQA
CHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
QCQFTAQRFDTLIPALRFKKFDAVIAGMEVIPMREKQVAFSRPYRQALSGVVLVNKDVAH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCEEEECHHHHH
SFADLKAKKVGVVKGTLHQRYLRDKQKAVQAVPYDDVASALAALKAGQITGVMGDFATLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
AWQQENPDYAIMDERATDPAYYGKQYAIAVRKDDPELLNAINDALAAVMATPDFQQMQQK
HHHCCCCCEEEECCCCCCCHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WFK
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]