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The map label for this gene is galS [H]

Identifier: 238892543

GI number: 238892543

Start: 327945

End: 328958

Strand: Reverse

Name: galS [H]

Synonym: KP1_0315

Alternate gene names: 238892543

Gene position: 328958-327945 (Counterclockwise)

Preceding gene: 238892544

Following gene: 238892542

Centisome position: 6.27

GC content: 64.69

Gene sequence:

>1014_bases
ATGAACCCACGCAAACCCAGCGTCACCGCCCAGGATGTTGCCCGGCGAGCGGGCGTCTCCCGGGCAGTGGTGTCCCGGGC
GTTGAGCAACAACGGCAGCATCTCTCCCGACGCCCGGGCGCGGGTACTGCGCGCCGCCGAAGAGCTGGGGTATCAGGTTA
ACTTCCTCGCCCAGGGGCTGAACCGTCAGCGCAGCCACTTAATCGGCGTGATCGTCTCGCGCATCAGCGATCCTTTTCGC
AGTACCCTGCTTGATGCGCTGCTTAACGAGATCCAGCGCCAGGGCTTTCAGGCGCTGGTGAGCGAGATCCACAGCGAGCA
GGATCTGGCGCAGACCTTGCGCCGTTTCGCTCAGTTTCGCGTCTCCGGCGTTATCGTCACCTCCGGCCAGCCACCGGAGG
CGCTGGTGAACGAGTGCGTCCAGCAGCATATTCCGGTCGTGGGCATTAATCGTCAGCCGACGATCCCCGGTGTGGATTAC
GTTTGTTCTGACAACGCCGCCGGCGCCGAACTGGCCGCCGACCAGCTGCTGCGCAGCGGCTGTCAGCGCTTTGGCTGGCT
CAACCACCATCCTTCCACCTGGGCCGGCCGCATGCGCGGGGAAGCGTTCGGCCGGGCGCTGCAAACGCGCGGAGTGGACG
TCGAACGCCACCTGGCGATCCTCGCCTGTCAGCAAGAAGGTTACGCGGGCGGGTTACAGGCTGCGGCGCTCGCCGACGAG
GCGCTGGAGGGGATCTTCTGCGCCAACGCCCAGCTTGCCTGTGGCTTTCTCGATGGAATGCGCCAGCGCGGCAGAGAGGC
TCCGGCGGATTTTCAGCTGATTGGTTTTGATAACACGCCGACGACCGCGCAATATAGCTATCAGCTCACCACGCTACATC
AGGATGTGGCGGCGATCGCCCGCCAGGCGCTCGCCCGGCTTCTGGAGCGCGCCGCCGACCCTTCGCAACCTTCACGCACC
ACCTGGGTGGAGGTAACCTTAATTCACCGGCGCACGTCGCCCTTTGTTATCTGA

Upstream 100 bases:

>100_bases
TAAAGCAGGCCTGTCAGCCGTGGCCGGGCTTATTCGTTTCCCGGGATGGACTCACCTTAACGCTGCCGCGCCCCACGCCC
ACTGACGAGACGTACCTCCT

Downstream 100 bases:

>100_bases
GAGCAGGACTATGACGGAAGCACAACGAGAGTCATTATGCGCCTTGATTCGCCAGGCAGGCGCCCACGCCCAGGCGCTGC
GCGATGCGGGATTACAGGTC

Product: LacI family sugar binding transcriptional regulator

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNPRKPSVTAQDVARRAGVSRAVVSRALSNNGSISPDARARVLRAAEELGYQVNFLAQGLNRQRSHLIGVIVSRISDPFR
STLLDALLNEIQRQGFQALVSEIHSEQDLAQTLRRFAQFRVSGVIVTSGQPPEALVNECVQQHIPVVGINRQPTIPGVDY
VCSDNAAGAELAADQLLRSGCQRFGWLNHHPSTWAGRMRGEAFGRALQTRGVDVERHLAILACQQEGYAGGLQAAALADE
ALEGIFCANAQLACGFLDGMRQRGREAPADFQLIGFDNTPTTAQYSYQLTTLHQDVAAIARQALARLLERAADPSQPSRT
TWVEVTLIHRRTSPFVI

Sequences:

>Translated_337_residues
MNPRKPSVTAQDVARRAGVSRAVVSRALSNNGSISPDARARVLRAAEELGYQVNFLAQGLNRQRSHLIGVIVSRISDPFR
STLLDALLNEIQRQGFQALVSEIHSEQDLAQTLRRFAQFRVSGVIVTSGQPPEALVNECVQQHIPVVGINRQPTIPGVDY
VCSDNAAGAELAADQLLRSGCQRFGWLNHHPSTWAGRMRGEAFGRALQTRGVDVERHLAILACQQEGYAGGLQAAALADE
ALEGIFCANAQLACGFLDGMRQRGREAPADFQLIGFDNTPTTAQYSYQLTTLHQDVAAIARQALARLLERAADPSQPSRT
TWVEVTLIHRRTSPFVI
>Mature_337_residues
MNPRKPSVTAQDVARRAGVSRAVVSRALSNNGSISPDARARVLRAAEELGYQVNFLAQGLNRQRSHLIGVIVSRISDPFR
STLLDALLNEIQRQGFQALVSEIHSEQDLAQTLRRFAQFRVSGVIVTSGQPPEALVNECVQQHIPVVGINRQPTIPGVDY
VCSDNAAGAELAADQLLRSGCQRFGWLNHHPSTWAGRMRGEAFGRALQTRGVDVERHLAILACQQEGYAGGLQAAALADE
ALEGIFCANAQLACGFLDGMRQRGREAPADFQLIGFDNTPTTAQYSYQLTTLHQDVAAIARQALARLLERAADPSQPSRT
TWVEVTLIHRRTSPFVI

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=31.8452380952381, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=29.5180722891566, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790194, Length=323, Percent_Identity=25.077399380805, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=27.190332326284, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787906, Length=291, Percent_Identity=31.2714776632302, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787580, Length=192, Percent_Identity=34.8958333333333, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=24.702380952381, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=24.1042345276873, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=25.2906976744186, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994940, Length=281, Percent_Identity=23.4875444839858, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790192, Length=234, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=22.9299363057325, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786268, Length=322, Percent_Identity=21.4285714285714, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36780; Mature: 36780

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRKPSVTAQDVARRAGVSRAVVSRALSNNGSISPDARARVLRAAEELGYQVNFLAQGL
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
NRQRSHLIGVIVSRISDPFRSTLLDALLNEIQRQGFQALVSEIHSEQDLAQTLRRFAQFR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VSGVIVTSGQPPEALVNECVQQHIPVVGINRQPTIPGVDYVCSDNAAGAELAADQLLRSG
HCEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
CQRFGWLNHHPSTWAGRMRGEAFGRALQTRGVDVERHLAILACQQEGYAGGLQAAALADE
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCHHHHHHHHH
ALEGIFCANAQLACGFLDGMRQRGREAPADFQLIGFDNTPTTAQYSYQLTTLHQDVAAIA
HHHHEHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHH
RQALARLLERAADPSQPSRTTWVEVTLIHRRTSPFVI
HHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNPRKPSVTAQDVARRAGVSRAVVSRALSNNGSISPDARARVLRAAEELGYQVNFLAQGL
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
NRQRSHLIGVIVSRISDPFRSTLLDALLNEIQRQGFQALVSEIHSEQDLAQTLRRFAQFR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VSGVIVTSGQPPEALVNECVQQHIPVVGINRQPTIPGVDYVCSDNAAGAELAADQLLRSG
HCEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
CQRFGWLNHHPSTWAGRMRGEAFGRALQTRGVDVERHLAILACQQEGYAGGLQAAALADE
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCHHHHHHHHH
ALEGIFCANAQLACGFLDGMRQRGREAPADFQLIGFDNTPTTAQYSYQLTTLHQDVAAIA
HHHHEHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHH
RQALARLLERAADPSQPSRTTWVEVTLIHRRTSPFVI
HHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019 [H]