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The map label for this gene is yjbN [H]

Identifier: 238892522

GI number: 238892522

Start: 304183

End: 305178

Strand: Direct

Name: yjbN [H]

Synonym: KP1_0290

Alternate gene names: 238892522

Gene position: 304183-305178 (Clockwise)

Preceding gene: 238892521

Following gene: 238892523

Centisome position: 5.8

GC content: 60.34

Gene sequence:

>996_bases
ATGCCTGAATCAACGTCACCTGTTTTCCCTGCTCACCGCTTCTCCATCGCGCCGATGCTCGACTGGACCGACCGACACTG
CCGCTACTTCCTGCGGCTGCTGTCGCGGCATACGCTGCTGTATACCGAAATGGTGACCACCGGGGCGATTATTCACGGCA
AAGGGGACTATCTGGCCTATAGCGAAGAAGAGCACCCGGTTGCTCTGCAGCTCGGCGGCAGCGATCCCCAGGCCCTGGCG
CAGTGCGCGAAGCTGGCCGAGGCGCGCGGCTATGATGAGATCAACCTCAACGTCGGCTGCCCCTCCGACCGCGTGCAGAA
CGGGATGTTTGGCGCTTGCCTGATGGGCAATGCGCCACTGGTGGCCGACTGCATCAAGGCGATGCGCGATGTGGTGTCGA
TTCCGGTCACGGTGAAAACCCGTATCGGCATTGACGACCAGGATAGCTATGAGTTTCTGTGCGATTTCATTGAGACGGTC
TCCGGCAAGGGCGAGTGTGAGATGTTTATTATTCACGCCCGCAAAGCCTGGCTCTCCGGCCTGAGTCCAAAAGAGAACCG
CGAGATCCCGCCGCTGGATTACCCCCGCGTCTGGCAGCTGAAGCGCGATTTCCCCCACCTTACGATGGCGATCAACGGCG
GCATCAAATCGCTGGACGAAGCCAGAGCACAGCTTGAGCATATGGATGGGGTGATGGTCGGGCGTGAGGCCTATCAGAAC
CCGGGCATTCTGGCCTCGGTCGACCGTGAAATTTTCGGCGTCGCCGGCGCGGATGCCGATCCGGTAGCGGTGGTGCGAGC
CATGTACCCCTATATTGAGCGCGAGCTCAGCAAGGGCACCTATCTGGGCCATATTACCCGCCATATGCTGGGGCTGTTTC
AGGGGATCCCCGGCGCTCGCCAGTGGCGTCGCTACCTGAGCGAAAATGCGCATAAAGCCGGCGCCGATATTGCGGTGCTG
GAGCATGCCCTGAAGCTGGTGGCAGATAAACGTTAA

Upstream 100 bases:

>100_bases
TTTCAGGTGAGGGGCGAAGCGCGGCGCACTATGCGCCTGTGGTGTATCTTTATGCTATAGTAGCGCCCCTTTTTACCCGG
ATGCTTATTTATTCGCCATG

Downstream 100 bases:

>100_bases
TTTCTGACCAAAACCTGGTGAAATTGACCACGCCTCGCGCACCGTCGCGGGGCGTTTTGTTTTAAAGTCAAAAGCTTATG
GTTGGCATGCTTTTTGTAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MPESTSPVFPAHRFSIAPMLDWTDRHCRYFLRLLSRHTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPQALA
QCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAPLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIETV
SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVWQLKRDFPHLTMAINGGIKSLDEARAQLEHMDGVMVGREAYQN
PGILASVDREIFGVAGADADPVAVVRAMYPYIERELSKGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADIAVL
EHALKLVADKR

Sequences:

>Translated_331_residues
MPESTSPVFPAHRFSIAPMLDWTDRHCRYFLRLLSRHTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPQALA
QCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAPLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIETV
SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVWQLKRDFPHLTMAINGGIKSLDEARAQLEHMDGVMVGREAYQN
PGILASVDREIFGVAGADADPVAVVRAMYPYIERELSKGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADIAVL
EHALKLVADKR
>Mature_330_residues
PESTSPVFPAHRFSIAPMLDWTDRHCRYFLRLLSRHTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPQALAQ
CAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAPLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIETVS
GKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVWQLKRDFPHLTMAINGGIKSLDEARAQLEHMDGVMVGREAYQNP
GILASVDREIFGVAGADADPVAVVRAMYPYIERELSKGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADIAVLE
HALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=24.025974025974, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=331, Percent_Identity=92.1450151057402, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=24.896265560166, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788462, Length=314, Percent_Identity=25.1592356687898, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.1410788381743, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=306, Percent_Identity=23.8562091503268, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=306, Percent_Identity=23.8562091503268, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36810; Mature: 36679

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESTSPVFPAHRFSIAPMLDWTDRHCRYFLRLLSRHTLLYTEMVTTGAIIHGKGDYLAY
CCCCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEE
SEEEHPVALQLGGSDPQALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAPL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCH
VADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIETVSGKGECEMFIIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCC
LSPKENREIPPLDYPRVWQLKRDFPHLTMAINGGIKSLDEARAQLEHMDGVMVGREAYQN
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCC
PGILASVDREIFGVAGADADPVAVVRAMYPYIERELSKGTYLGHITRHMLGLFQGIPGAR
CCEEEECCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
QWRRYLSENAHKAGADIAVLEHALKLVADKR
HHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PESTSPVFPAHRFSIAPMLDWTDRHCRYFLRLLSRHTLLYTEMVTTGAIIHGKGDYLAY
CCCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEE
SEEEHPVALQLGGSDPQALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAPL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCH
VADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIETVSGKGECEMFIIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCC
LSPKENREIPPLDYPRVWQLKRDFPHLTMAINGGIKSLDEARAQLEHMDGVMVGREAYQN
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCC
PGILASVDREIFGVAGADADPVAVVRAMYPYIERELSKGTYLGHITRHMLGLFQGIPGAR
CCEEEECCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHH
QWRRYLSENAHKAGADIAVLEHALKLVADKR
HHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]