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The map label for this gene is thiH [H]

Identifier: 238892464

GI number: 238892464

Start: 240008

End: 241141

Strand: Reverse

Name: thiH [H]

Synonym: KP1_0229

Alternate gene names: 238892464

Gene position: 241141-240008 (Counterclockwise)

Preceding gene: 238892465

Following gene: 238892463

Centisome position: 4.59

GC content: 61.99

Gene sequence:

>1134_bases
ATGAAAACCTTTAGCGATCGCTGGCGGCAGCTGGAGTGGGACGATATCCGCCTGCGCATCAACAGCAAAACCGCCGCCGA
CGTCGAACGGGCGCTGGCGGCTAAACAGCTCACCCGCGACGATATGATGGCCCTGCTCTCGCCTGCCGCGGCCAATTACC
TTGAACCGCTGGCCCAGCGCGCGCAGCGCCTCACCCGGCAGCGCTTCGGCAATACCGTCAGCTTCTACGTGCCGCTGTAT
CTCTCCAACCTGTGCGCTAACGACTGCACCTACTGCGGCTTCTCGATGAGCAACCGCATTAAGCGCAAGACGCTGGATGC
CGCCGAGATTGCCCGCGAGTGCGCCGCGATCCGCAACCTGGGCTTTGAGCATCTGCTGCTGGTGACCGGCGAACATCAGG
GTAAGGTGGGAATGGATTATTTCCGTCAGCACCTACCGGCTATCCGCAGCCAGTTTGCCTCGCTGCATATGGAGGTGCAG
CCGCTGGCGACTGAAGAGTATGCCGAGCTGAAAACCCTGGGACTCGACGGGGTGATGGTCTATCAGGAGACCTATCATGA
AAGCATGTACGCCCAACACCATCTGAAAGGAAAAAAACAGGATTTCTTCTGGCGGCTGGATACGCCGGATCGCTTAGGCG
CGGCGGGGATCGATAAGATCGGCCTTGGCGCGCTCATCGGCCTTTCCGACAGCTGGCGGGTGGACTGCTTTATAGTCGCT
GAGCACCTGCTGTGGCTGCAGCAGCGCTACTGGCGCAGCCGCTATTCGGTCTCCTTCCCGCGCCTGCGTCCCTGCGCCGG
CGGTATTGAACCGGCTTCGCTGATGGATGAGCGCCAGCTGGTGCAAACTATCTGCGCCTTCCGCCTGCTGGCGCCAGAGG
TTGAGCTGTCGCTATCCACCCGCGAATCACCCTGGTTCCGCGACCGGGTGATCCCGCTGGCGATCAATAACGTCAGCGCC
TTCTCTAAAACCCAGCCCGGCGGCTACGCCGACGATCATCCGGAGCTCGAGCAATTTGCCCCGCACGACGATCGCCGCCC
TGAAGAGGTCGCCAGCGCGCTGGCCGCCCGCGGCCTGCAGCCGGTATGGAAAGACTGGGATAGCTGGCTGGGACGCGCTT
CGCAAACCTCATGA

Upstream 100 bases:

>100_bases
CGATCGATGCCGGATTACTGGCGCGTCAGGCCGGACCGGGGGCACGCAGTACGCAGGCGCAGGCCACCAGCCCGCTCACC
GGATTCCTGGAGGCGCTGGC

Downstream 100 bases:

>100_bases
TGTACAGTGTAAACGCCTGATAATTTTGCGCGCCTGCACGAGAGATGAAAAACAGAGGCTGGCCCGCCCGACGCCGTTTT
TCTATTCTGCCCTGGTCAGC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKTFSDRWRQLEWDDIRLRINSKTAADVERALAAKQLTRDDMMALLSPAAANYLEPLAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDAAEIARECAAIRNLGFEHLLLVTGEHQGKVGMDYFRQHLPAIRSQFASLHMEVQ
PLATEEYAELKTLGLDGVMVYQETYHESMYAQHHLKGKKQDFFWRLDTPDRLGAAGIDKIGLGALIGLSDSWRVDCFIVA
EHLLWLQQRYWRSRYSVSFPRLRPCAGGIEPASLMDERQLVQTICAFRLLAPEVELSLSTRESPWFRDRVIPLAINNVSA
FSKTQPGGYADDHPELEQFAPHDDRRPEEVASALAARGLQPVWKDWDSWLGRASQTS

Sequences:

>Translated_377_residues
MKTFSDRWRQLEWDDIRLRINSKTAADVERALAAKQLTRDDMMALLSPAAANYLEPLAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDAAEIARECAAIRNLGFEHLLLVTGEHQGKVGMDYFRQHLPAIRSQFASLHMEVQ
PLATEEYAELKTLGLDGVMVYQETYHESMYAQHHLKGKKQDFFWRLDTPDRLGAAGIDKIGLGALIGLSDSWRVDCFIVA
EHLLWLQQRYWRSRYSVSFPRLRPCAGGIEPASLMDERQLVQTICAFRLLAPEVELSLSTRESPWFRDRVIPLAINNVSA
FSKTQPGGYADDHPELEQFAPHDDRRPEEVASALAARGLQPVWKDWDSWLGRASQTS
>Mature_377_residues
MKTFSDRWRQLEWDDIRLRINSKTAADVERALAAKQLTRDDMMALLSPAAANYLEPLAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDAAEIARECAAIRNLGFEHLLLVTGEHQGKVGMDYFRQHLPAIRSQFASLHMEVQ
PLATEEYAELKTLGLDGVMVYQETYHESMYAQHHLKGKKQDFFWRLDTPDRLGAAGIDKIGLGALIGLSDSWRVDCFIVA
EHLLWLQQRYWRSRYSVSFPRLRPCAGGIEPASLMDERQLVQTICAFRLLAPEVELSLSTRESPWFRDRVIPLAINNVSA
FSKTQPGGYADDHPELEQFAPHDDRRPEEVASALAARGLQPVWKDWDSWLGRASQTS

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=375, Percent_Identity=88.8, Blast_Score=704, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43069; Mature: 43069

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFSDRWRQLEWDDIRLRINSKTAADVERALAAKQLTRDDMMALLSPAAANYLEPLAQR
CCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDAAEIARECAAIRNL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHC
GFEHLLLVTGEHQGKVGMDYFRQHLPAIRSQFASLHMEVQPLATEEYAELKTLGLDGVMV
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCEEH
YQETYHESMYAQHHLKGKKQDFFWRLDTPDRLGAAGIDKIGLGALIGLSDSWRVDCFIVA
HHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHCCHHEEECCCCCCCHHHHHH
EHLLWLQQRYWRSRYSVSFPRLRPCAGGIEPASLMDERQLVQTICAFRLLAPEVELSLST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDRVIPLAINNVSAFSKTQPGGYADDHPELEQFAPHDDRRPEEVASALAARGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQTS
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTFSDRWRQLEWDDIRLRINSKTAADVERALAAKQLTRDDMMALLSPAAANYLEPLAQR
CCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDAAEIARECAAIRNL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHC
GFEHLLLVTGEHQGKVGMDYFRQHLPAIRSQFASLHMEVQPLATEEYAELKTLGLDGVMV
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCEEH
YQETYHESMYAQHHLKGKKQDFFWRLDTPDRLGAAGIDKIGLGALIGLSDSWRVDCFIVA
HHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHCCHHEEECCCCCCCHHHHHH
EHLLWLQQRYWRSRYSVSFPRLRPCAGGIEPASLMDERQLVQTICAFRLLAPEVELSLST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDRVIPLAINNVSAFSKTQPGGYADDHPELEQFAPHDDRRPEEVASALAARGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQTS
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]