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The map label for this gene is dpnM [H]

Identifier: 339905975

GI number: 339905975

Start: 3081003

End: 3081788

Strand: Reverse

Name: dpnM [H]

Synonym: NA

Alternate gene names: 339905975

Gene position: 3081788-3081003 (Counterclockwise)

Preceding gene: 238028252

Following gene: 238028250

Centisome position: 78.89

GC content: 60.43

Gene sequence:

>786_bases
ATGGCGGAACCCATCATTCCCTGGCTCGGCGGCAAGCGCCGTCTGGCAGACATCCTGATTCCCCGCTTTCCTGCGCACGA
GTGCTACGTCGAGGTGTTCGCGGGCGGGGCGGCGCTGTACTTCCTTCGGCCGCCGGCCAAGGTCGAGGTGATCAACGACA
TCAATGGGGAACTGATCAACCTGTATCGGGTGGTGCAGCACCATCTGGAGGAGTTTGTACGCCAGTTCAAATGGGCGCTG
ACGAGCCGTCAGGTGTTTGAATGGCTCAAGCAGACAGTCCCGGAAAGTCTCACCGATATCCAGCGTGCGGCGAGGTTCTA
TTACCTGCAGAAAAGTTGCTTTGGCGGCAAGCTGGAGGGGCAGACGTTCGGCACGAGGACGGCCGCCCCGCCGAGCTTGA
ACCTGATCCGGCTGGAAGAGGATCTGTCGGCGGCACATCTGCGGCTCTCTGGCGCGTACATCGAGCGTCTGGATTGGGCC
GAGTGCATCGATCGATACGATCGCCCGCATACGCTGTTCTACTTGGATCCGCCGTATCTCGACACGGCCGGCTATGGCGT
AGCGTTTCTGTCCAATCAATACGAGAAGATGGCCGAGCGTCTACGGACGCTCAAGGGCCGTGCGATCGTGAGCCTGAATG
ATCACCCGGAGATCCGGCGCGTTTTCGATGGATTCCATATCGAAACAGTGCCGATCCAGTACACGGTTGGCCTGGAGGCC
GCAAGCCGCCGCGAGGTGATCATTTCGAGCTGGGACGTCGGCGCAGAGCCGGCTGGGTTGTTCTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:
NA

Sequences:

>Translated_261_residues
MAEPIIPWLGGKRRLADILIPRFPAHECYVEVFAGGAALYFLRPPAKVEVINDINGELINLYRVVQHHLEEFVRQFKWAL
TSRQVFEWLKQTVPESLTDIQRAARFYYLQKSCFGGKLEGQTFGTRTAAPPSLNLIRLEEDLSAAHLRLSGAYIERLDWA
ECIDRYDRPHTLFYLDPPYLDTAGYGVAFLSNQYEKMAERLRTLKGRAIVSLNDHPEIRRVFDGFHIETVPIQYTVGLEA
ASRREVIISSWDVGAEPAGLF
>Mature_260_residues
AEPIIPWLGGKRRLADILIPRFPAHECYVEVFAGGAALYFLRPPAKVEVINDINGELINLYRVVQHHLEEFVRQFKWALT
SRQVFEWLKQTVPESLTDIQRAARFYYLQKSCFGGKLEGQTFGTRTAAPPSLNLIRLEEDLSAAHLRLSGAYIERLDWAE
CIDRYDRPHTLFYLDPPYLDTAGYGVAFLSNQYEKMAERLRTLKGRAIVSLNDHPEIRRVFDGFHIETVPIQYTVGLEAA
SRREVIISSWDVGAEPAGLF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=265, Percent_Identity=26.0377358490566, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 29886; Mature: 29754

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPIIPWLGGKRRLADILIPRFPAHECYVEVFAGGAALYFLRPPAKVEVINDINGELIN
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHEEECCEEEEEECCCCEEEEECCCCHHHHH
LYRVVQHHLEEFVRQFKWALTSRQVFEWLKQTVPESLTDIQRAARFYYLQKSCFGGKLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QTFGTRTAAPPSLNLIRLEEDLSAAHLRLSGAYIERLDWAECIDRYDRPHTLFYLDPPYL
CCCCCCCCCCCCCEEEEECCCCCHHHEEECHHHHHHCCHHHHHHHCCCCCEEEEECCCCC
DTAGYGVAFLSNQYEKMAERLRTLKGRAIVSLNDHPEIRRVFDGFHIETVPIQYTVGLEA
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEEEEEEEEECCCC
ASRREVIISSWDVGAEPAGLF
CCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AEPIIPWLGGKRRLADILIPRFPAHECYVEVFAGGAALYFLRPPAKVEVINDINGELIN
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHEEECCEEEEEECCCCEEEEECCCCHHHHH
LYRVVQHHLEEFVRQFKWALTSRQVFEWLKQTVPESLTDIQRAARFYYLQKSCFGGKLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QTFGTRTAAPPSLNLIRLEEDLSAAHLRLSGAYIERLDWAECIDRYDRPHTLFYLDPPYL
CCCCCCCCCCCCCEEEEECCCCCHHHEEECHHHHHHCCHHHHHHHCCCCCEEEEECCCCC
DTAGYGVAFLSNQYEKMAERLRTLKGRAIVSLNDHPEIRRVFDGFHIETVPIQYTVGLEA
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEEEEEEEEECCCC
ASRREVIISSWDVGAEPAGLF
CCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]