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The map label for this gene is rsuA [H]

Identifier: 238028391

GI number: 238028391

Start: 3237124

End: 3237843

Strand: Direct

Name: rsuA [H]

Synonym: bglu_1g28550

Alternate gene names: 238028391

Gene position: 3237124-3237843 (Clockwise)

Preceding gene: 238028390

Following gene: 238028392

Centisome position: 82.86

GC content: 69.44

Gene sequence:

>720_bases
ATGAACCTCGAAACCATCCTCTTTTCGCAGGGCTTCGGCTCGCGCCGCCAATGCCGCGCGCTCGTCGCCGCGGGACGCGT
CGCGATCGGCGGCGCGATCTGCGACGATCCCGAGCAGTCCTTTCCCACCGACGGCCTCGAATTCGACGTCGACGGCACGC
GCTGGCTCTACCGACAGCACGCCTACCTCGTGCTGAACAAGCCGGCCGGCTACGAATGCTCGCGCGAGCCGCAGCACCAC
GAAAGCGTGTTCGCGCTGCTGCCGCCGCAGCTCGCCACCCGCGGCGTGCAATGCGTGGGCCGGCTCGACCAGGACACCAC
GGGTCTGCTGCTGCTATCCGACGACGGCCAGTTCGTGCATGCCTACACCTCGCCGAAACGCAAGGTCCCGAAGACCTATC
TCGCCACCCTCAAGCATCCGCTCGACGCCGCGCAACTGACCGCGCTGCGCGACGGCGTACTGCTGCACGGCGAACCGAAG
CCGATCGCCGCGCTCGACGCGCAGGCGCGCGGCACGCACCAGCTCGAAATGACCGTGCAGGAAGGGAAATACCATCAGGT
GAAGCGGATGATCGCCGCCGCGGGCAACCGCTGCGAGCAATTGCACCGCGAGCGGATCGGCGGCTTCGCGCTGCCGGCCG
ACGCGCTGCCGCCCGGTGCCTGGCGCTGGCTAGACGAGGCCGACCTCGCCTCGTTGCGTGCCGCCGCGCCGGCACGCTGA

Upstream 100 bases:

>100_bases
GACATAGTTCCGGGAAATTCCCGGAAGTCCCAAAACCCGCGACAGCACAACGCTTCGCGGGTTTTTTCATGTCCTTCGCC
CTTGCTACAATGCGCGCGCC

Downstream 100 bases:

>100_bases
ACGCGCCTACCCCGGCACCGCGCGCGCGTCGATCCGCACAATCCCCGCCGTTGCGCCCACGCACGGCGCGCTGCGGCGCA
ATGTGGAACTCCGCTGCGCC

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNLETILFSQGFGSRRQCRALVAAGRVAIGGAICDDPEQSFPTDGLEFDVDGTRWLYRQHAYLVLNKPAGYECSREPQHH
ESVFALLPPQLATRGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYLATLKHPLDAAQLTALRDGVLLHGEPK
PIAALDAQARGTHQLEMTVQEGKYHQVKRMIAAAGNRCEQLHRERIGGFALPADALPPGAWRWLDEADLASLRAAAPAR

Sequences:

>Translated_239_residues
MNLETILFSQGFGSRRQCRALVAAGRVAIGGAICDDPEQSFPTDGLEFDVDGTRWLYRQHAYLVLNKPAGYECSREPQHH
ESVFALLPPQLATRGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYLATLKHPLDAAQLTALRDGVLLHGEPK
PIAALDAQARGTHQLEMTVQEGKYHQVKRMIAAAGNRCEQLHRERIGGFALPADALPPGAWRWLDEADLASLRAAAPAR
>Mature_239_residues
MNLETILFSQGFGSRRQCRALVAAGRVAIGGAICDDPEQSFPTDGLEFDVDGTRWLYRQHAYLVLNKPAGYECSREPQHH
ESVFALLPPQLATRGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYLATLKHPLDAAQLTALRDGVLLHGEPK
PIAALDAQARGTHQLEMTVQEGKYHQVKRMIAAAGNRCEQLHRERIGGFALPADALPPGAWRWLDEADLASLRAAAPAR

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=240, Percent_Identity=38.75, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=33.4728033472803, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=25.531914893617, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081838, Length=149, Percent_Identity=30.8724832214765, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26237; Mature: 26237

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLETILFSQGFGSRRQCRALVAAGRVAIGGAICDDPEQSFPTDGLEFDVDGTRWLYRQH
CCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCHHCCCCCCCEECCCCHHEEEECC
AYLVLNKPAGYECSREPQHHESVFALLPPQLATRGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYLATLKHPLDAAQLTALRDGVLLHGEPKPIAALDAQARGTHQLEMTVQ
EECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCEEECCCCCCCEEEEEEEC
EGKYHQVKRMIAAAGNRCEQLHRERIGGFALPADALPPGAWRWLDEADLASLRAAAPAR
CCCHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCCCCCCHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLETILFSQGFGSRRQCRALVAAGRVAIGGAICDDPEQSFPTDGLEFDVDGTRWLYRQH
CCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCHHCCCCCCCEECCCCHHEEEECC
AYLVLNKPAGYECSREPQHHESVFALLPPQLATRGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYLATLKHPLDAAQLTALRDGVLLHGEPKPIAALDAQARGTHQLEMTVQ
EECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCEEECCCCCCCEEEEEEEC
EGKYHQVKRMIAAAGNRCEQLHRERIGGFALPADALPPGAWRWLDEADLASLRAAAPAR
CCCHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCCCCCCHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]