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The map label for this gene is recE [H]

Identifier: 238027671

GI number: 238027671

Start: 2281352

End: 2282158

Strand: Reverse

Name: recE [H]

Synonym: bglu_1g20920

Alternate gene names: 238027671

Gene position: 2282158-2281352 (Counterclockwise)

Preceding gene: 238027672

Following gene: 238027670

Centisome position: 58.42

GC content: 63.57

Gene sequence:

>807_bases
ATGCTCGAATACAACCTGTCGATCGAGGCGTATCACGCCATGGCGCCGGTGAGCAAGTCGCAGCTGGATGACATCGACGT
GTCTCCGTTCCATTTCTATGCGCGGCACCGCGATCCCAACCGGCCGGCGCGCGAGACGAAGTCTGGGCAACTGGAGGGCC
AGCTTGCGCATTGCGCGATCCTCGAGCCTGACGAGTTCGGCAAGCGCTACGTCATCGGCCCGAGCGTGCGCCGGAACACG
AAGGTGTGGACGGAGTTCGTCGAAGCTCATCCGGACCGCATTGCGATCCAGCAGGACCAAGCCGACGTCGCATGGCGTCA
ATCGGATTCGGTTCGCGCGCTACCTGACGTGCGCGATGCACTGTCCCGCGGCCACGCTGAAGTGTCGGCATTCTGGACCG
ATCCCGAGACGGGTGTCGAGTGCCGTTGCCGGCCCGACTTCGCGCATGACCTCGACAACACGTCCGTGGTGCTGCTCGAC
GTGAAGACGTTCAGTAGCGCTGACCCCGACGAATTCCGTCGCCAGGCCGCACGCAAGCGCTATCACGTCCAGGCGGCGTA
CTACACGGACGGATATGCCGCTGCGTCCGGCCTGGACGTCGCTGCGTTCATTTTCGTCGCAGTCGAGACGGAATGGCCCT
ACGCGGCCAGTGCCGTGATGCTCGACCAGATTTCGCTCGAGCAAGGCCGCGCGGACTACCGACGCAACCTCGCCACCTAC
GCACGCTGCTCGGCAGCTAACGAGTGGCCCGGCTACTCGCGCGATATTTCCCTCATCTCTTTGCCCCAATGGGCCTTTCA
GGACTGA

Upstream 100 bases:

>100_bases
TCGCACTGCTCCTGTTCCTCTGGCTCGTGCTGCCGAATCTCGGTGTGTCGCACGAGTCGGCGCCGCAACCCATTCAACCC
ACGTCTGCGAAGGTGGCGTG

Downstream 100 bases:

>100_bases
ACCATGAACGATCTCGTCGCATCCCCCTTCGGTGGCCGCAGCACGGCCGTTGCTGATACCGCCGGGGCTCGCCAGGACCA
GAGCCGCGAGCTCGCGGAAA

Product: Exonuclease VIII, 5' - 3' specific dsDNA exonuclease

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLEYNLSIEAYHAMAPVSKSQLDDIDVSPFHFYARHRDPNRPARETKSGQLEGQLAHCAILEPDEFGKRYVIGPSVRRNT
KVWTEFVEAHPDRIAIQQDQADVAWRQSDSVRALPDVRDALSRGHAEVSAFWTDPETGVECRCRPDFAHDLDNTSVVLLD
VKTFSSADPDEFRRQAARKRYHVQAAYYTDGYAAASGLDVAAFIFVAVETEWPYAASAVMLDQISLEQGRADYRRNLATY
ARCSAANEWPGYSRDISLISLPQWAFQD

Sequences:

>Translated_268_residues
MLEYNLSIEAYHAMAPVSKSQLDDIDVSPFHFYARHRDPNRPARETKSGQLEGQLAHCAILEPDEFGKRYVIGPSVRRNT
KVWTEFVEAHPDRIAIQQDQADVAWRQSDSVRALPDVRDALSRGHAEVSAFWTDPETGVECRCRPDFAHDLDNTSVVLLD
VKTFSSADPDEFRRQAARKRYHVQAAYYTDGYAAASGLDVAAFIFVAVETEWPYAASAVMLDQISLEQGRADYRRNLATY
ARCSAANEWPGYSRDISLISLPQWAFQD
>Mature_268_residues
MLEYNLSIEAYHAMAPVSKSQLDDIDVSPFHFYARHRDPNRPARETKSGQLEGQLAHCAILEPDEFGKRYVIGPSVRRNT
KVWTEFVEAHPDRIAIQQDQADVAWRQSDSVRALPDVRDALSRGHAEVSAFWTDPETGVECRCRPDFAHDLDNTSVVLLD
VKTFSSADPDEFRRQAARKRYHVQAAYYTDGYAAASGLDVAAFIFVAVETEWPYAASAVMLDQISLEQGRADYRRNLATY
ARCSAANEWPGYSRDISLISLPQWAFQD

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=278, Percent_Identity=35.9712230215827, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 30336; Mature: 30336

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYNLSIEAYHAMAPVSKSQLDDIDVSPFHFYARHRDPNRPARETKSGQLEGQLAHCAI
CEEEECEEEEEHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
LEPDEFGKRYVIGPSVRRNTKVWTEFVEAHPDRIAIQQDQADVAWRQSDSVRALPDVRDA
ECHHHHCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHH
LSRGHAEVSAFWTDPETGVECRCRPDFAHDLDNTSVVLLDVKTFSSADPDEFRRQAARKR
HHCCCCEEEEEECCCCCCCEEEECCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
YHVQAAYYTDGYAAASGLDVAAFIFVAVETEWPYAASAVMLDQISLEQGRADYRRNLATY
EEEEEEEEECCCHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
ARCSAANEWPGYSRDISLISLPQWAFQD
HHHCCCCCCCCCCCCEEEEECCCHHCCC
>Mature Secondary Structure
MLEYNLSIEAYHAMAPVSKSQLDDIDVSPFHFYARHRDPNRPARETKSGQLEGQLAHCAI
CEEEECEEEEEHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
LEPDEFGKRYVIGPSVRRNTKVWTEFVEAHPDRIAIQQDQADVAWRQSDSVRALPDVRDA
ECHHHHCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHH
LSRGHAEVSAFWTDPETGVECRCRPDFAHDLDNTSVVLLDVKTFSSADPDEFRRQAARKR
HHCCCCEEEEEECCCCCCCEEEECCCHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
YHVQAAYYTDGYAAASGLDVAAFIFVAVETEWPYAASAVMLDQISLEQGRADYRRNLATY
EEEEEEEEECCCHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
ARCSAANEWPGYSRDISLISLPQWAFQD
HHHCCCCCCCCCCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]