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The map label for this gene is cheR [C]

Identifier: 238026818

GI number: 238026818

Start: 1291452

End: 1292300

Strand: Reverse

Name: cheR [C]

Synonym: bglu_1g11770

Alternate gene names: 238026818

Gene position: 1292300-1291452 (Counterclockwise)

Preceding gene: 238026819

Following gene: 238026817

Centisome position: 33.08

GC content: 66.55

Gene sequence:

>849_bases
ATGCAACCCCACCCGCCCGTTCCGCCGCCGCGGCACACGGCCGATTTCGATATCGAGCTGAAGCTGCTGCTCGAGGCGAT
CTACCAGAAGTACCAGCACGATTTCCGCCACTATGCGCTGTCCTCGCTGCGCCGCCGTCTGAGCCAGGCGCTGACCGAAT
TCGGCCTGCCCACCCTCTCGCAGCTGCAGGACAGAATCCTGCGCAGCGAGGCCGACTTCACGCGCCTGTTCCAGTACCTG
ACGGTGCAGGTCAGCGACATGTTCCGCGATCCGCCCTACTTCCTCGCGCTGCGCGAGCACGTGCTGCCGCTCCTGCAGAC
CTATCCGTCGATCAAGGTCTGGGTGGCCGGCTGCTGCACCGGCGAGGAGCTGTGGTCGCTGAAGATCCTGTTCGACGAGG
AAGGGCTGACCGAGCGAACGCTGTTCTATGCCACCGACATCAATCCCGACGCGCTCGCGCGCGCCGAGGCGGGCATCTAC
ACGCTCGACCGGATCGCGGGATTCTCGCGCAACTATCTGGCCGCGGGCGGCACGCGCTCGCTGTCCGATTACTATCACGC
GGCCTACGGCGGCGCGCGCTTCTCCGCCTCGCTGCGCGAGCGCGTGGTGTTCGCCGACCACAGCCTCTCGACCGACGAGG
TCTTCCTCGAAGCTCATCTGGTGTCGTGCCGCAACGTGCTGATCTACTTCGACCGTGCGCTGCAGGACCGTGCGCTGGCG
CTGTTCGAGCGCAGCCTCGTGCGGCGCGGCTTCCTCGGGCTCGGCAGCAAGGAAAGCCTGCGCTTCTCCGCCCGTCCCGA
GGCGTTCGGCGAGTTCCGCGAGCGCGAACGCATCTACCAGAAACGCTAG

Upstream 100 bases:

>100_bases
AGCCGGCGCGGACGACTACATCTCGAAGCCGATCGACGTCGACCGGCTGCTGTCGCTGTGCCGCGTCTGGTTCCGGCAGC
GCTGACGCAAAGGGCAACCC

Downstream 100 bases:

>100_bases
CGCCCATGGCCCCGTTCCCCCTGCCTCGCGTGCCCTCGGGCACCGCCCGGAGCCGCGCGTGAGCCGCCCGCCGCCCCTCT
CCCCGCAGCGCCGGCGCGGG

Product: CheR-type MCP methyltransferase

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQPHPPVPPPRHTADFDIELKLLLEAIYQKYQHDFRHYALSSLRRRLSQALTEFGLPTLSQLQDRILRSEADFTRLFQYL
TVQVSDMFRDPPYFLALREHVLPLLQTYPSIKVWVAGCCTGEELWSLKILFDEEGLTERTLFYATDINPDALARAEAGIY
TLDRIAGFSRNYLAAGGTRSLSDYYHAAYGGARFSASLRERVVFADHSLSTDEVFLEAHLVSCRNVLIYFDRALQDRALA
LFERSLVRRGFLGLGSKESLRFSARPEAFGEFRERERIYQKR

Sequences:

>Translated_282_residues
MQPHPPVPPPRHTADFDIELKLLLEAIYQKYQHDFRHYALSSLRRRLSQALTEFGLPTLSQLQDRILRSEADFTRLFQYL
TVQVSDMFRDPPYFLALREHVLPLLQTYPSIKVWVAGCCTGEELWSLKILFDEEGLTERTLFYATDINPDALARAEAGIY
TLDRIAGFSRNYLAAGGTRSLSDYYHAAYGGARFSASLRERVVFADHSLSTDEVFLEAHLVSCRNVLIYFDRALQDRALA
LFERSLVRRGFLGLGSKESLRFSARPEAFGEFRERERIYQKR
>Mature_282_residues
MQPHPPVPPPRHTADFDIELKLLLEAIYQKYQHDFRHYALSSLRRRLSQALTEFGLPTLSQLQDRILRSEADFTRLFQYL
TVQVSDMFRDPPYFLALREHVLPLLQTYPSIKVWVAGCCTGEELWSLKILFDEEGLTERTLFYATDINPDALARAEAGIY
TLDRIAGFSRNYLAAGGTRSLSDYYHAAYGGARFSASLRERVVFADHSLSTDEVFLEAHLVSCRNVLIYFDRALQDRALA
LFERSLVRRGFLGLGSKESLRFSARPEAFGEFRERERIYQKR

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 32583; Mature: 32583

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00092 N6_MTASE ; PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPHPPVPPPRHTADFDIELKLLLEAIYQKYQHDFRHYALSSLRRRLSQALTEFGLPTLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLQDRILRSEADFTRLFQYLTVQVSDMFRDPPYFLALREHVLPLLQTYPSIKVWVAGCCT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GEELWSLKILFDEEGLTERTLFYATDINPDALARAEAGIYTLDRIAGFSRNYLAAGGTRS
CHHHEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCH
LSDYYHAAYGGARFSASLRERVVFADHSLSTDEVFLEAHLVSCRNVLIYFDRALQDRALA
HHHHHHHHHCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFERSLVRRGFLGLGSKESLRFSARPEAFGEFRERERIYQKR
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPHPPVPPPRHTADFDIELKLLLEAIYQKYQHDFRHYALSSLRRRLSQALTEFGLPTLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLQDRILRSEADFTRLFQYLTVQVSDMFRDPPYFLALREHVLPLLQTYPSIKVWVAGCCT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GEELWSLKILFDEEGLTERTLFYATDINPDALARAEAGIYTLDRIAGFSRNYLAAGGTRS
CHHHEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCH
LSDYYHAAYGGARFSASLRERVVFADHSLSTDEVFLEAHLVSCRNVLIYFDRALQDRALA
HHHHHHHHHCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFERSLVRRGFLGLGSKESLRFSARPEAFGEFRERERIYQKR
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]