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The map label for this gene is rpoN [H]

Identifier: 238026814

GI number: 238026814

Start: 1287807

End: 1289261

Strand: Direct

Name: rpoN [H]

Synonym: bglu_1g11730

Alternate gene names: 238026814

Gene position: 1287807-1289261 (Clockwise)

Preceding gene: 238026812

Following gene: 238026815

Centisome position: 32.97

GC content: 69.69

Gene sequence:

>1455_bases
ATGCCCGCTACGCTCGCGCTACAGATGCGGCAACACCTGACGCTGACGCCTCGGCTGCAACAGTCGCTGCGATTGCTGCA
GTTGTCGTCGCTCGAATTCCAGCAGGAACTGCGTCAGGCGCTCGACACCAACCCGTTTCTCGAGGACGTGCAGATTTCAG
GCGACGACGACACGCGCACGCCGTTCGACGAAGCGACCGCCGCGGCCGAATCGGCCGAGACGTCGGAGCCCGCGAGCCTC
GTCACCGCCGAACCGGGCCCGCGCGACGGCGAGCTTTCCTATACGGCCGAACCGTCGATGCGCGGCTCGCTGCGCCGCTC
CAACGAGAGCTTCGACGTCGAGCCGTCGGACCGCCTCGTGCTCGAACCGTCCCTGCACGAACACCTGCACGAGGCGCTCT
GCCTCTATCCGCTCAACACGCGTGACCGCGCCGCCGCGCGCACCATCATCGACGCGCTCGACGACGACGGCTACCTGCGC
CAGTCGCTGGCCGAGCTGGCCGCCACGATCGCCGATTCGGACGCCTGCTTCAGCGAACAGGAACTCGCGGTAGCGCTGCG
GCTCGTGCAGTCGCTCGACAAGCCGGGGCTCGCGGCGCGCTCGCTGTCCGAGTGCCTGATGCTGCAGTTGAACGCGATGC
CGGGCGGGCGCGACGACGTGGAGTGCGCGAAGGTGATCGCCGAGCATCATCTCGAACGGCTCGCGCGCCGCGAGACGGCC
GAGATCCAGCGCCGCATCGGCTGCGATACGCGCACGCTCCGCGCGGCCTGCGCGCTGGTGCGCAAGCTCGATCCGCGCCC
GGGCAACCATTTCGGCGGCGCGAGCGGCGACTACGTGGTGCCCGACGTGATCGTGCGGCAGGTGAGCGGGCGCTGGGTGG
TGACGATCAACCCGGCGGTGATGCCGCGCGCGCGCATCCACAAGCTCTATGCCGAGCTGTTCGCGAAGTCGAGCGAATCG
GCCCAGTCGCCGCTCGGCCAGCAGCTGCAGGAGGCACGCTGGCTGATCCGCAATGCGCAGAAGCGCTTCGACACGATCCA
GCGCGTGGGCGAATGCATCGTCGCGCGGCAGCGCGACTTCTTCCGTTACGGTGCGATCGCGCTGAAGCCGCTGGTGCTGC
GCGACATCGCCGAGGAGCTGGATCTGCACGAATCGACCATCTCGCGCGCCACCGGCAACAAGTACATGGCCACGCCGCAC
GGCACCTTCGAGTTCAAGCACTTCTTCCCGCGCAAGCTCGAGGCGGCGGGGCGCGGCGCCTGCTCGGCGGCGGCGGCCAA
GGTGCTGATCCGCGAGATGATCGCGGCCGAGCCCGGCGGCGAACCGCTTTCCGACGTGGCGCTGGCGCAGCGCCTGGCCG
GCCGCGGCGTGGTGCTGGCGCGGCGCACCGTCACCAAGTACCGGCAGGCCATGAAGATCCTGCCGGCCGAGTTGCGCCGG
CGCGTGCCGGTGTGA

Upstream 100 bases:

>100_bases
GGCGCAGCCCTGTCGGGCCGGGCGCGGGCGGGCCGAGGGCAGGCTGGTATGGATGTTGCAAAAGGAGAGGCAGGACCACT
TAACCGACAGGAGCATCCGG

Downstream 100 bases:

>100_bases
GGCGCGCGGCGGCCGTGCCGCCGTGCGGCGACGGTTTCACAATTCCAAACTGGAGGTAGACATGCTTCGATACGCGGTCA
TCTTTTTCATCATCGCCATC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPATLALQMRQHLTLTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQISGDDDTRTPFDEATAAAESAETSEPASL
VTAEPGPRDGELSYTAEPSMRGSLRRSNESFDVEPSDRLVLEPSLHEHLHEALCLYPLNTRDRAAARTIIDALDDDGYLR
QSLAELAATIADSDACFSEQELAVALRLVQSLDKPGLAARSLSECLMLQLNAMPGGRDDVECAKVIAEHHLERLARRETA
EIQRRIGCDTRTLRAACALVRKLDPRPGNHFGGASGDYVVPDVIVRQVSGRWVVTINPAVMPRARIHKLYAELFAKSSES
AQSPLGQQLQEARWLIRNAQKRFDTIQRVGECIVARQRDFFRYGAIALKPLVLRDIAEELDLHESTISRATGNKYMATPH
GTFEFKHFFPRKLEAAGRGACSAAAAKVLIREMIAAEPGGEPLSDVALAQRLAGRGVVLARRTVTKYRQAMKILPAELRR
RVPV

Sequences:

>Translated_484_residues
MPATLALQMRQHLTLTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQISGDDDTRTPFDEATAAAESAETSEPASL
VTAEPGPRDGELSYTAEPSMRGSLRRSNESFDVEPSDRLVLEPSLHEHLHEALCLYPLNTRDRAAARTIIDALDDDGYLR
QSLAELAATIADSDACFSEQELAVALRLVQSLDKPGLAARSLSECLMLQLNAMPGGRDDVECAKVIAEHHLERLARRETA
EIQRRIGCDTRTLRAACALVRKLDPRPGNHFGGASGDYVVPDVIVRQVSGRWVVTINPAVMPRARIHKLYAELFAKSSES
AQSPLGQQLQEARWLIRNAQKRFDTIQRVGECIVARQRDFFRYGAIALKPLVLRDIAEELDLHESTISRATGNKYMATPH
GTFEFKHFFPRKLEAAGRGACSAAAAKVLIREMIAAEPGGEPLSDVALAQRLAGRGVVLARRTVTKYRQAMKILPAELRR
RVPV
>Mature_483_residues
PATLALQMRQHLTLTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQISGDDDTRTPFDEATAAAESAETSEPASLV
TAEPGPRDGELSYTAEPSMRGSLRRSNESFDVEPSDRLVLEPSLHEHLHEALCLYPLNTRDRAAARTIIDALDDDGYLRQ
SLAELAATIADSDACFSEQELAVALRLVQSLDKPGLAARSLSECLMLQLNAMPGGRDDVECAKVIAEHHLERLARRETAE
IQRRIGCDTRTLRAACALVRKLDPRPGNHFGGASGDYVVPDVIVRQVSGRWVVTINPAVMPRARIHKLYAELFAKSSESA
QSPLGQQLQEARWLIRNAQKRFDTIQRVGECIVARQRDFFRYGAIALKPLVLRDIAEELDLHESTISRATGNKYMATPHG
TFEFKHFFPRKLEAAGRGACSAAAAKVLIREMIAAEPGGEPLSDVALAQRLAGRGVVLARRTVTKYRQAMKILPAELRRR
VPV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=485, Percent_Identity=37.319587628866, Blast_Score=315, Evalue=6e-87,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53635; Mature: 53504

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATLALQMRQHLTLTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQISGDDDTRT
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCEECCCCCCCC
PFDEATAAAESAETSEPASLVTAEPGPRDGELSYTAEPSMRGSLRRSNESFDVEPSDRLV
CHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCEE
LEPSLHEHLHEALCLYPLNTRDRAAARTIIDALDDDGYLRQSLAELAATIADSDACFSEQ
ECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCHH
ELAVALRLVQSLDKPGLAARSLSECLMLQLNAMPGGRDDVECAKVIAEHHLERLARRETA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIQRRIGCDTRTLRAACALVRKLDPRPGNHFGGASGDYVVPDVIVRQVSGRWVVTINPAV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
MPRARIHKLYAELFAKSSESAQSPLGQQLQEARWLIRNAQKRFDTIQRVGECIVARQRDF
CCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRYGAIALKPLVLRDIAEELDLHESTISRATGNKYMATPHGTFEFKHFFPRKLEAAGRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCHHHHHHCCCCH
CSAAAAKVLIREMIAAEPGGEPLSDVALAQRLAGRGVVLARRTVTKYRQAMKILPAELRR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
RVPV
CCCC
>Mature Secondary Structure 
PATLALQMRQHLTLTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQISGDDDTRT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCEECCCCCCCC
PFDEATAAAESAETSEPASLVTAEPGPRDGELSYTAEPSMRGSLRRSNESFDVEPSDRLV
CHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCEE
LEPSLHEHLHEALCLYPLNTRDRAAARTIIDALDDDGYLRQSLAELAATIADSDACFSEQ
ECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCHH
ELAVALRLVQSLDKPGLAARSLSECLMLQLNAMPGGRDDVECAKVIAEHHLERLARRETA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIQRRIGCDTRTLRAACALVRKLDPRPGNHFGGASGDYVVPDVIVRQVSGRWVVTINPAV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
MPRARIHKLYAELFAKSSESAQSPLGQQLQEARWLIRNAQKRFDTIQRVGECIVARQRDF
CCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRYGAIALKPLVLRDIAEELDLHESTISRATGNKYMATPHGTFEFKHFFPRKLEAAGRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCHHHHHHCCCCH
CSAAAAKVLIREMIAAEPGGEPLSDVALAQRLAGRGVVLARRTVTKYRQAMKILPAELRR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
RVPV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]