Click here to switch to the map view.

The map label for this gene is flbD [H]

Identifier: 238026805

GI number: 238026805

Start: 1276100

End: 1277083

Strand: Reverse

Name: flbD [H]

Synonym: bglu_1g11630

Alternate gene names: 238026805

Gene position: 1277083-1276100 (Counterclockwise)

Preceding gene: 238026813

Following gene: 238026802

Centisome position: 32.69

GC content: 69.92

Gene sequence:

>984_bases
ATGTCGAGACTCACTAGCGAAGGCCGGCGTCTTGCCGGCTCCTCGCCGCGCATCCAGGCGCTGCTGTCGAAGCTCGAGAA
GATTGCCGCGACCCGCGCGAGCGTGCTGATCGTCGGCGAGAGCGGCGCGGGCAAGGACATCGCCGCGCGCCTCGTCCACG
ATTCGAGCCCGCGCGCGAACGGCCCGTTCGTCGCCGTGAATTGCGGCGCGATCCCGGCCGATATCGCCGAATCGCAGCTG
TTCGGCCACGAGAAGGGCAGCTTCACCGGCGCGAGCGCGCAGCATATCGGCTTCTTCGAGGCCGCGCGCGGCGGCACGAT
CTTCCTCGACGAAATCGCCGAGATGCCGCGCGACCTGCAGGTCAAGCTGCTGCGCGCGCTCGAATCGAACACCATCATGC
GCGTGGGCGGCACCGAGACGATCGCGCTCGACGTGCGGGTGGTGGCCGCCACGCATCACAATCCGGCCGATGCGATCCGC
GACGGCCGCCTGCGCGAGGACCTGTTCTACCGGCTCGCGGCGATGGCGCTGCACGTGCCGGCGCTGCGCCATCGCGACGG
CGACGTCGAGGAGATCGCGCGCGCGCTGATCGACACGCTCAACACGCACCACGGCACCCACAAGGCGCTCTCGGCGCAGG
CGCTCAAGCTGCTGCGCAGCTACCCGTGGCCCGGCAACGTGCGCGAGCTGCGCAACACCATCGAGCGCGCGTTCATCCTG
GCCGACGACACCATCGAACTCGATCTGCCGCGCCAGCTGCCGCCGCGCGCCGACGTGCGCGACAACGTGATGTCGCTGCC
GCTCGGCGCGACGCTCGCCAATGCGCAGCAGCGCTTCATCATGGCCTCGCTGCGCCACTTCGACGGCGACAAGCCGCGCA
CGGCAAAGGCGCTCGGCATCAGCCTGAAAACGCTCTACAACCGGCTCGCGCTGATGCGCGAGCACGGCATGCAGCCCGAG
CTCGACGAGGGCGCGCGCCGCTAG

Upstream 100 bases:

>100_bases
ATTAAGTTCGTCAGGATTCGATACGCTTGCGGTTATAAAAATTACACGCTACGCGGCGAGCGCCCATCCAGGCGCCTCCT
TTCTACCATGAGAACCATCC

Downstream 100 bases:

>100_bases
GCACGCGCCGCCCGTCGTCAGCGCCGCGGCGGGTCCGCTGCGGGGCCGGCCGGCCCCGCCGGCGGCTCGGCCTCGCCCGG
TCCGAGCGCGCCGCACGGAT

Product: Sigma-54 interaction domain/Fis family transcriptional regulator domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSRLTSEGRRLAGSSPRIQALLSKLEKIAATRASVLIVGESGAGKDIAARLVHDSSPRANGPFVAVNCGAIPADIAESQL
FGHEKGSFTGASAQHIGFFEAARGGTIFLDEIAEMPRDLQVKLLRALESNTIMRVGGTETIALDVRVVAATHHNPADAIR
DGRLREDLFYRLAAMALHVPALRHRDGDVEEIARALIDTLNTHHGTHKALSAQALKLLRSYPWPGNVRELRNTIERAFIL
ADDTIELDLPRQLPPRADVRDNVMSLPLGATLANAQQRFIMASLRHFDGDKPRTAKALGISLKTLYNRLALMREHGMQPE
LDEGARR

Sequences:

>Translated_327_residues
MSRLTSEGRRLAGSSPRIQALLSKLEKIAATRASVLIVGESGAGKDIAARLVHDSSPRANGPFVAVNCGAIPADIAESQL
FGHEKGSFTGASAQHIGFFEAARGGTIFLDEIAEMPRDLQVKLLRALESNTIMRVGGTETIALDVRVVAATHHNPADAIR
DGRLREDLFYRLAAMALHVPALRHRDGDVEEIARALIDTLNTHHGTHKALSAQALKLLRSYPWPGNVRELRNTIERAFIL
ADDTIELDLPRQLPPRADVRDNVMSLPLGATLANAQQRFIMASLRHFDGDKPRTAKALGISLKTLYNRLALMREHGMQPE
LDEGARR
>Mature_326_residues
SRLTSEGRRLAGSSPRIQALLSKLEKIAATRASVLIVGESGAGKDIAARLVHDSSPRANGPFVAVNCGAIPADIAESQLF
GHEKGSFTGASAQHIGFFEAARGGTIFLDEIAEMPRDLQVKLLRALESNTIMRVGGTETIALDVRVVAATHHNPADAIRD
GRLREDLFYRLAAMALHVPALRHRDGDVEEIARALIDTLNTHHGTHKALSAQALKLLRSYPWPGNVRELRNTIERAFILA
DDTIELDLPRQLPPRADVRDNVMSLPLGATLANAQQRFIMASLRHFDGDKPRTAKALGISLKTLYNRLALMREHGMQPEL
DEGARR

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=314, Percent_Identity=41.0828025477707, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=41.1764705882353, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI87082117, Length=228, Percent_Identity=46.4912280701754, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=37.8378378378378, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=37.4213836477987, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=43.9655172413793, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=37.6175548589342, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=38.5802469135802, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=45.7627118644068, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=35.9133126934984, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=34.1692789968652, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789828, Length=280, Percent_Identity=30.3571428571429, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=30.2721088435374, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35751; Mature: 35620

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLTSEGRRLAGSSPRIQALLSKLEKIAATRASVLIVGESGAGKDIAARLVHDSSPRAN
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
GPFVAVNCGAIPADIAESQLFGHEKGSFTGASAQHIGFFEAARGGTIFLDEIAEMPRDLQ
CCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCEEEHHHHHCCCHHHH
VKLLRALESNTIMRVGGTETIALDVRVVAATHHNPADAIRDGRLREDLFYRLAAMALHVP
HHHHHHHHCCCEEEECCCEEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ALRHRDGDVEEIARALIDTLNTHHGTHKALSAQALKLLRSYPWPGNVRELRNTIERAFIL
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEE
ADDTIELDLPRQLPPRADVRDNVMSLPLGATLANAQQRFIMASLRHFDGDKPRTAKALGI
ECCEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLKTLYNRLALMREHGMQPELDEGARR
HHHHHHHHHHHHHHCCCCCCHHCCCCC
>Mature Secondary Structure 
SRLTSEGRRLAGSSPRIQALLSKLEKIAATRASVLIVGESGAGKDIAARLVHDSSPRAN
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
GPFVAVNCGAIPADIAESQLFGHEKGSFTGASAQHIGFFEAARGGTIFLDEIAEMPRDLQ
CCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCEEEHHHHHCCCHHHH
VKLLRALESNTIMRVGGTETIALDVRVVAATHHNPADAIRDGRLREDLFYRLAAMALHVP
HHHHHHHHCCCEEEECCCEEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ALRHRDGDVEEIARALIDTLNTHHGTHKALSAQALKLLRSYPWPGNVRELRNTIERAFIL
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEE
ADDTIELDLPRQLPPRADVRDNVMSLPLGATLANAQQRFIMASLRHFDGDKPRTAKALGI
ECCEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLKTLYNRLALMREHGMQPELDEGARR
HHHHHHHHHHHHHHCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]