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The map label for this gene is argT [H]

Identifier: 238026663

GI number: 238026663

Start: 1095781

End: 1096578

Strand: Direct

Name: argT [H]

Synonym: bglu_1g10160

Alternate gene names: 238026663

Gene position: 1095781-1096578 (Clockwise)

Preceding gene: 238026662

Following gene: 238026666

Centisome position: 28.05

GC content: 67.67

Gene sequence:

>798_bases
ATGAAGTTGAACTGGCGAAAGATGGCCGCATGCGCGGTGGCGACGGCCGCCGCGGTGTCCTTCCTGTCGGGCGCGCACGC
GGCCGAGCTCAAGCAGATCCGTTTCGGCGTCGAGGCGTCGTATGCGCCGTTCGAATACAAGACGCCCGACGGCAAGCTCG
CCGGTTTCGACGTCGACGTCGGCAATGCGGTCTGCGCGAAGCTGAAGGTGCCGTGCGTGTGGGTGGAGAACGACTTCGAC
GGCCTGATCCCGGCGCTGCAGGCGCGCAAGTTCGACGCGATCAACTCCGACATGACGATCACGCCGCAGCGCCTGCACGC
GATCGCGTTCACCGATCCGATCTACTCGATCCCGAACCAGCTGATCGCGAAGAAGGGCAGCGGCCTGCTGCCGACGCCGG
CCTCGCTAAAGGGCAAGCGGGTGGGCGTGCTGCAGGGCACGATCCAGGAGGCCTACGCGAAGGCGCGCTGGGCGCCGGCC
GGCGTCGAGGTGGTGCCCTACCAGACCCAGGATCTGGCCTACGCCGACCTGCAGTCGGGCCGGCTCGACGCGACCTTCCA
GGATTCGGAGGCGGCCGCCAAGGGCTTCCTGTCGAAGCCGCAGGCAGCCGGGTTCGCGTTCGCCGGCCCGCAGGTGGTGG
ACGCCGAGATCCTCGGCTCGGGCGTCGGCTACGGCCTGCGCAAGAACGACACGGCGCTGCGCGCCGCGCTCAACCAGGCG
CTCAAGGAGCTGAAGGCGGACGGTACGATCGACGCAATCGCGAAGAAGTATTTCAGCGTGCCGGTGGTGCTGAAGTAA

Upstream 100 bases:

>100_bases
GCGCCGCCCGCGCGTTTGGTCCGGACGGCGTGCCCACGAGGCGCGTGTGATCCGGTTGTCGTAGCACCCTTCCTGAAGAA
AGAGCGAGGAGAGCATTCCA

Downstream 100 bases:

>100_bases
GCGGGCCGCGGGCGCGGGCGGCCGCCAGGCTCGCCCGGCGGCCGCGCCAAGGCGGCCGGGCCGGCGCTTCCCGGGCAAGG
GGGGGCGCCGGCTTTTTTTC

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKLNWRKMAACAVATAAAVSFLSGAHAAELKQIRFGVEASYAPFEYKTPDGKLAGFDVDVGNAVCAKLKVPCVWVENDFD
GLIPALQARKFDAINSDMTITPQRLHAIAFTDPIYSIPNQLIAKKGSGLLPTPASLKGKRVGVLQGTIQEAYAKARWAPA
GVEVVPYQTQDLAYADLQSGRLDATFQDSEAAAKGFLSKPQAAGFAFAGPQVVDAEILGSGVGYGLRKNDTALRAALNQA
LKELKADGTIDAIAKKYFSVPVVLK

Sequences:

>Translated_265_residues
MKLNWRKMAACAVATAAAVSFLSGAHAAELKQIRFGVEASYAPFEYKTPDGKLAGFDVDVGNAVCAKLKVPCVWVENDFD
GLIPALQARKFDAINSDMTITPQRLHAIAFTDPIYSIPNQLIAKKGSGLLPTPASLKGKRVGVLQGTIQEAYAKARWAPA
GVEVVPYQTQDLAYADLQSGRLDATFQDSEAAAKGFLSKPQAAGFAFAGPQVVDAEILGSGVGYGLRKNDTALRAALNQA
LKELKADGTIDAIAKKYFSVPVVLK
>Mature_265_residues
MKLNWRKMAACAVATAAAVSFLSGAHAAELKQIRFGVEASYAPFEYKTPDGKLAGFDVDVGNAVCAKLKVPCVWVENDFD
GLIPALQARKFDAINSDMTITPQRLHAIAFTDPIYSIPNQLIAKKGSGLLPTPASLKGKRVGVLQGTIQEAYAKARWAPA
GVEVVPYQTQDLAYADLQSGRLDATFQDSEAAAKGFLSKPQAAGFAFAGPQVVDAEILGSGVGYGLRKNDTALRAALNQA
LKELKADGTIDAIAKKYFSVPVVLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=49.609375, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=50, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=37.8048780487805, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787088, Length=238, Percent_Identity=35.2941176470588, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=32.1428571428571, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28161; Mature: 28161

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNWRKMAACAVATAAAVSFLSGAHAAELKQIRFGVEASYAPFEYKTPDGKLAGFDVDV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEC
GNAVCAKLKVPCVWVENDFDGLIPALQARKFDAINSDMTITPQRLHAIAFTDPIYSIPNQ
CCHHHHEEECCEEEEECCCCHHHHHHHHHHHHCCCCCCEECHHHHEEEEECCCHHHHHHH
LIAKKGSGLLPTPASLKGKRVGVLQGTIQEAYAKARWAPAGVEVVPYQTQDLAYADLQSG
HHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCC
RLDATFQDSEAAAKGFLSKPQAAGFAFAGPQVVDAEILGSGVGYGLRKNDTALRAALNQA
CCCCEECCHHHHHHHHCCCCCCCCEEECCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHH
LKELKADGTIDAIAKKYFSVPVVLK
HHHHCCCCCHHHHHHHHHCCCEEEC
>Mature Secondary Structure
MKLNWRKMAACAVATAAAVSFLSGAHAAELKQIRFGVEASYAPFEYKTPDGKLAGFDVDV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEC
GNAVCAKLKVPCVWVENDFDGLIPALQARKFDAINSDMTITPQRLHAIAFTDPIYSIPNQ
CCHHHHEEECCEEEEECCCCHHHHHHHHHHHHCCCCCCEECHHHHEEEEECCCHHHHHHH
LIAKKGSGLLPTPASLKGKRVGVLQGTIQEAYAKARWAPAGVEVVPYQTQDLAYADLQSG
HHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCC
RLDATFQDSEAAAKGFLSKPQAAGFAFAGPQVVDAEILGSGVGYGLRKNDTALRAALNQA
CCCCEECCHHHHHHHHCCCCCCCCEEECCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHH
LKELKADGTIDAIAKKYFSVPVVLK
HHHHCCCCCHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]