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The map label for this gene is otsB [H]

Identifier: 238026636

GI number: 238026636

Start: 1061082

End: 1061834

Strand: Direct

Name: otsB [H]

Synonym: bglu_1g09880

Alternate gene names: 238026636

Gene position: 1061082-1061834 (Clockwise)

Preceding gene: 238026633

Following gene: 238026637

Centisome position: 27.16

GC content: 71.05

Gene sequence:

>753_bases
ATGCAATCCGTCCCGGCTTCCCTCCCCCTGACCGATACCGCATTCTTCTTCGATTTCGACGGCACGCTCGTCGATCTCGC
GCCGACGCCAGACGGCATTCACGTACCCGCCTCGCTGCCCGCGCTGCTGACCGAGCTGCGCACGCTCACGCGCGGCGCGG
TGGCGATCGTCTCGGGCCGCGGCATCGACAGCATCGACGGCTTCCTCGGCCTCACGGACCTGCCGGTCGCCGGGCTGCAC
GGCGCCGAGCGGCGCGACGCGAACGGCGATACGCAGCGCGTGGGCTTCAACGACGAGCGCCTGCTGCGCATCGAGCACGA
GCTCGCCGCGGTGGTCGAGCGTCATCCCGGCATGCTGCTCGAGATCAAGGGCGCGGCCGTCGCGCTGCATTTCCGCAACG
CCCCCGAACGCGAGGCGGTGGCGCGCGAGGCGGCCGAGCGGCTCGTGGCCGACTATCCCGATGCCTACGTGCTGCAGCCC
GGCAAGATGGTGTTCGAGATCAAGCCGAAAGGCGTCGACAAGGGCCGCGCGATCGCGGCGTTCCTGAACGAGCCGCCGTT
CGCGGGCCGCAGGCCGGTGTTCGCCGGCGACGACCTGACCGACGAGAAGGGCTTCGCGGTGGTGGATGCGGCCGGCGGCC
TGTCGATCAAGATTGGCGCGGGCGATACCACGGCGCGCGCACGGCTCGATTCGGTCGAGGCGATGCGCGCGCAGATCGCC
GCCTGGATTGCGGCGGAGCCCGCCCGCGCATGA

Upstream 100 bases:

>100_bases
GCCCTCGGCCCGGCCGGCGCGGTAAGATGCCGTCATGCTTTCGTCGGCCGCTGCGCCGGCGGCGGCCAGCCATGCCCACG
CCACCCTCACCCGAGAACCG

Downstream 100 bases:

>100_bases
GCCGGCTGATCATCGTATCGAATCGGGTCGCGCCGATCTCCGAGGGCGAACCGGCCGCGGGCGGCCTCGCTATCGGCGTC
TACGACGCGCTCAAGGAAAC

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQSVPASLPLTDTAFFFDFDGTLVDLAPTPDGIHVPASLPALLTELRTLTRGAVAIVSGRGIDSIDGFLGLTDLPVAGLH
GAERRDANGDTQRVGFNDERLLRIEHELAAVVERHPGMLLEIKGAAVALHFRNAPEREAVAREAAERLVADYPDAYVLQP
GKMVFEIKPKGVDKGRAIAAFLNEPPFAGRRPVFAGDDLTDEKGFAVVDAAGGLSIKIGAGDTTARARLDSVEAMRAQIA
AWIAAEPARA

Sequences:

>Translated_250_residues
MQSVPASLPLTDTAFFFDFDGTLVDLAPTPDGIHVPASLPALLTELRTLTRGAVAIVSGRGIDSIDGFLGLTDLPVAGLH
GAERRDANGDTQRVGFNDERLLRIEHELAAVVERHPGMLLEIKGAAVALHFRNAPEREAVAREAAERLVADYPDAYVLQP
GKMVFEIKPKGVDKGRAIAAFLNEPPFAGRRPVFAGDDLTDEKGFAVVDAAGGLSIKIGAGDTTARARLDSVEAMRAQIA
AWIAAEPARA
>Mature_250_residues
MQSVPASLPLTDTAFFFDFDGTLVDLAPTPDGIHVPASLPALLTELRTLTRGAVAIVSGRGIDSIDGFLGLTDLPVAGLH
GAERRDANGDTQRVGFNDERLLRIEHELAAVVERHPGMLLEIKGAAVALHFRNAPEREAVAREAAERLVADYPDAYVLQP
GKMVFEIKPKGVDKGRAIAAFLNEPPFAGRRPVFAGDDLTDEKGFAVVDAAGGLSIKIGAGDTTARARLDSVEAMRAQIA
AWIAAEPARA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=231, Percent_Identity=48.0519480519481, Blast_Score=201, Evalue=3e-53,
Organism=Drosophila melanogaster, GI221473368, Length=249, Percent_Identity=27.3092369477912, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129307, Length=249, Percent_Identity=27.3092369477912, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582490, Length=249, Percent_Identity=27.3092369477912, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26538; Mature: 26538

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVPASLPLTDTAFFFDFDGTLVDLAPTPDGIHVPASLPALLTELRTLTRGAVAIVSGR
CCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCHHHHHHHHHHHHHCCEEEEEECC
GIDSIDGFLGLTDLPVAGLHGAERRDANGDTQRVGFNDERLLRIEHELAAVVERHPGMLL
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHCCCEEE
EIKGAAVALHFRNAPEREAVAREAAERLVADYPDAYVLQPGKMVFEIKPKGVDKGRAIAA
EECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCEEEE
FLNEPPFAGRRPVFAGDDLTDEKGFAVVDAAGGLSIKIGAGDTTARARLDSVEAMRAQIA
ECCCCCCCCCCCEECCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
AWIAAEPARA
HHHHCCCCCC
>Mature Secondary Structure
MQSVPASLPLTDTAFFFDFDGTLVDLAPTPDGIHVPASLPALLTELRTLTRGAVAIVSGR
CCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCHHHHHHHHHHHHHCCEEEEEECC
GIDSIDGFLGLTDLPVAGLHGAERRDANGDTQRVGFNDERLLRIEHELAAVVERHPGMLL
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHCCCEEE
EIKGAAVALHFRNAPEREAVAREAAERLVADYPDAYVLQPGKMVFEIKPKGVDKGRAIAA
EECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCEEEE
FLNEPPFAGRRPVFAGDDLTDEKGFAVVDAAGGLSIKIGAGDTTARARLDSVEAMRAQIA
ECCCCCCCCCCCEECCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
AWIAAEPARA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]