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The map label for this gene is xylX [H]

Identifier: 238025433

GI number: 238025433

Start: 2631055

End: 2632443

Strand: Reverse

Name: xylX [H]

Synonym: bglu_2g21230

Alternate gene names: 238025433

Gene position: 2632443-2631055 (Counterclockwise)

Preceding gene: 238025434

Following gene: 238025432

Centisome position: 93.11

GC content: 63.5

Gene sequence:

>1389_bases
GTGAATGCAGGGAAAGTTACGTCGCAATGGCAGGAATTCGTCGAAGGATGCCTCGATTTCCGCCCGGACGAGGGCGTCTA
CCGCATTGCCCGAGACATGTTTACCGAGCCGCAACTGTTCGATCTGGAGATGGAGTTCATCTTCGAGAAGAACTGGATTT
ACGCGTGTCACGAGAGCGAGATTCCCCAGCCGCACGACTTCGTGACCATGCGGGCGGGCCGCCAGCCGCTGATCGTCTCG
CGCGGCGGCAACGGCGAGCTGAACGCCATGATCAATGCCTGCCAGCATCGCGGCGCAACGCTCACGCGCATGGGCAAGGG
CAACCAGTCCACCTTCACCTGTCCGTTTCATGCGTGGTGCTACAAGAGCGACGGGCGCCTCGTGAAGGTCAAGGCGCCGG
GCGAGTATGGCGAGGACTTCGACAAGTCCAAGCGGGGTCTCAAGAAGGCGCGCATCGCCAGCTACAAGGGCTTCGTGTTC
GTCAGCCTCGACGCGGCCGGCACCGACACGCTCGAAGACTATCTGGGCGACGCGCGCGTCTTCCTCGACATGATGGTGGC
GCAGTCGCCCACCGGCGAGCTGGAAGTGCTGCCGGGCAAGTCCACCTACACCTACGAGGGAAACTGGAAGCTGCAGAACG
AGAACGGCCTCGACGGCTATCACGTGAGTACCGTGCACTACAACTACGTCGCCACGGTGCAGCATCGGCAGGAGGCGAAC
GCGCGCAAGGGCGGCGCGACGGCCGGGACGCTCGACTACAGCAAGCTGGGCGCGGGCGATGCGGATACCGACGACGGCTG
GTTCGCGTTCCGCAACGGTCACAGCGTGCTGTTCAGCGACATGCCCAACCCGAGCGTGCGCCCCGGCTATGCCTCCGTCA
TGCCGCGCCTCGTGGCTGCCCACGGCCAGCAGAAGGCCGAGTGGATGATGCACCGGCTGCGCAACCTGAACGTCTATCCG
AGCCTGTTCTTCATGGACCAGATCAGTTCGCAGCTGCGCATCGTGCGCCCGGTCGCCTGGAACAAGACCGAAATCATCAG
CCAGTGCATCGGAGTGAAAGGCGAATCGGACAGCGACCGCGAAAGCCGCATTCGCCAGTTCGAGGACTTCTTCAACGTAT
CGGGAATGGGCACGCCCGACGATCTCGTGGAATTCCGCGAGCAGCAGCGCGGCTTCCAGGCGCGCCTCGAACGCTGGAGC
GACGTGTCGCGCGGGCATCAGAAATGGGTGGCGGGGGAGACGGCAAACACGCGCCTGCTCGGCATCGCCCCCGTGCTGTG
CGGTACCGAGCTGACCCATGAGGGGCTTTACGTCAACCAGCACGGCGCCTGGCAGAAGTTTCTGATGCAGGGGCTCGCAC
AACGATCGAATGCCGGGAAGGAGGATTGA

Upstream 100 bases:

>100_bases
AAGGCGATCAGGTGGATAGCGGCCGAATCGGCCCGGGTATTCAATCGAGTCCATCAAGGACCAGGCGGCAAGGCCGCCGA
GCAGCCGGAGGGTACCCATC

Downstream 100 bases:

>100_bases
ATCATGGCTGTCACGCATGCGCTGCAACAATCGGTCGAGCAGTTTCTGTACCGGATGGCCGAGCTTTGCGACGCCCAGAG
CTGGGACGAGTACCTCGACC

Product: anthranilate 1,2-dioxygenase subunit alpha

Products: catechol; CO2; NAD+; NADP+; NH3

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNAGKVTSQWQEFVEGCLDFRPDEGVYRIARDMFTEPQLFDLEMEFIFEKNWIYACHESEIPQPHDFVTMRAGRQPLIVS
RGGNGELNAMINACQHRGATLTRMGKGNQSTFTCPFHAWCYKSDGRLVKVKAPGEYGEDFDKSKRGLKKARIASYKGFVF
VSLDAAGTDTLEDYLGDARVFLDMMVAQSPTGELEVLPGKSTYTYEGNWKLQNENGLDGYHVSTVHYNYVATVQHRQEAN
ARKGGATAGTLDYSKLGAGDADTDDGWFAFRNGHSVLFSDMPNPSVRPGYASVMPRLVAAHGQQKAEWMMHRLRNLNVYP
SLFFMDQISSQLRIVRPVAWNKTEIISQCIGVKGESDSDRESRIRQFEDFFNVSGMGTPDDLVEFREQQRGFQARLERWS
DVSRGHQKWVAGETANTRLLGIAPVLCGTELTHEGLYVNQHGAWQKFLMQGLAQRSNAGKED

Sequences:

>Translated_462_residues
MNAGKVTSQWQEFVEGCLDFRPDEGVYRIARDMFTEPQLFDLEMEFIFEKNWIYACHESEIPQPHDFVTMRAGRQPLIVS
RGGNGELNAMINACQHRGATLTRMGKGNQSTFTCPFHAWCYKSDGRLVKVKAPGEYGEDFDKSKRGLKKARIASYKGFVF
VSLDAAGTDTLEDYLGDARVFLDMMVAQSPTGELEVLPGKSTYTYEGNWKLQNENGLDGYHVSTVHYNYVATVQHRQEAN
ARKGGATAGTLDYSKLGAGDADTDDGWFAFRNGHSVLFSDMPNPSVRPGYASVMPRLVAAHGQQKAEWMMHRLRNLNVYP
SLFFMDQISSQLRIVRPVAWNKTEIISQCIGVKGESDSDRESRIRQFEDFFNVSGMGTPDDLVEFREQQRGFQARLERWS
DVSRGHQKWVAGETANTRLLGIAPVLCGTELTHEGLYVNQHGAWQKFLMQGLAQRSNAGKED
>Mature_462_residues
MNAGKVTSQWQEFVEGCLDFRPDEGVYRIARDMFTEPQLFDLEMEFIFEKNWIYACHESEIPQPHDFVTMRAGRQPLIVS
RGGNGELNAMINACQHRGATLTRMGKGNQSTFTCPFHAWCYKSDGRLVKVKAPGEYGEDFDKSKRGLKKARIASYKGFVF
VSLDAAGTDTLEDYLGDARVFLDMMVAQSPTGELEVLPGKSTYTYEGNWKLQNENGLDGYHVSTVHYNYVATVQHRQEAN
ARKGGATAGTLDYSKLGAGDADTDDGWFAFRNGHSVLFSDMPNPSVRPGYASVMPRLVAAHGQQKAEWMMHRLRNLNVYP
SLFFMDQISSQLRIVRPVAWNKTEIISQCIGVKGESDSDRESRIRQFEDFFNVSGMGTPDDLVEFREQQRGFQARLERWS
DVSRGHQKWVAGETANTRLLGIAPVLCGTELTHEGLYVNQHGAWQKFLMQGLAQRSNAGKED

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=379, Percent_Identity=28.4960422163588, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788103, Length=213, Percent_Identity=30.5164319248826, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.12.1

Molecular weight: Translated: 52066; Mature: 52066

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGKVTSQWQEFVEGCLDFRPDEGVYRIARDMFTEPQLFDLEMEFIFEKNWIYACHESE
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCEEEEECCCC
IPQPHDFVTMRAGRQPLIVSRGGNGELNAMINACQHRGATLTRMGKGNQSTFTCPFHAWC
CCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEECCEEEE
YKSDGRLVKVKAPGEYGEDFDKSKRGLKKARIASYKGFVFVSLDAAGTDTLEDYLGDARV
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCHHHH
FLDMMVAQSPTGELEVLPGKSTYTYEGNWKLQNENGLDGYHVSTVHYNYVATVQHRQEAN
HHHHHHHCCCCCCEEEECCCCEEEECCCEEECCCCCCCCEEEEEEEEEEEEEEHHHHHCC
ARKGGATAGTLDYSKLGAGDADTDDGWFAFRNGHSVLFSDMPNPSVRPGYASVMPRLVAA
CCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
HGQQKAEWMMHRLRNLNVYPSLFFMDQISSQLRIVRPVAWNKTEIISQCIGVKGESDSDR
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCHH
ESRIRQFEDFFNVSGMGTPDDLVEFREQQRGFQARLERWSDVSRGHQKWVAGETANTRLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEE
GIAPVLCGTELTHEGLYVNQHGAWQKFLMQGLAQRSNAGKED
EEHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAGKVTSQWQEFVEGCLDFRPDEGVYRIARDMFTEPQLFDLEMEFIFEKNWIYACHESE
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCEEEEECCCC
IPQPHDFVTMRAGRQPLIVSRGGNGELNAMINACQHRGATLTRMGKGNQSTFTCPFHAWC
CCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEECCEEEE
YKSDGRLVKVKAPGEYGEDFDKSKRGLKKARIASYKGFVFVSLDAAGTDTLEDYLGDARV
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCHHHH
FLDMMVAQSPTGELEVLPGKSTYTYEGNWKLQNENGLDGYHVSTVHYNYVATVQHRQEAN
HHHHHHHCCCCCCEEEECCCCEEEECCCEEECCCCCCCCEEEEEEEEEEEEEEHHHHHCC
ARKGGATAGTLDYSKLGAGDADTDDGWFAFRNGHSVLFSDMPNPSVRPGYASVMPRLVAA
CCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
HGQQKAEWMMHRLRNLNVYPSLFFMDQISSQLRIVRPVAWNKTEIISQCIGVKGESDSDR
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCHH
ESRIRQFEDFFNVSGMGTPDDLVEFREQQRGFQARLERWSDVSRGHQKWVAGETANTRLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEE
GIAPVLCGTELTHEGLYVNQHGAWQKFLMQGLAQRSNAGKED
EEHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: anthranilate; NADH; NADPH; H+; O2

Specific reaction: anthranilate + NAD(P)H + 2 H+ + O2 = catechol + CO2 + NAD(P)+ + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]