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The map label for this gene is amsH [H]

Identifier: 238024993

GI number: 238024993

Start: 2073378

End: 2074601

Strand: Reverse

Name: amsH [H]

Synonym: bglu_2g16500

Alternate gene names: 238024993

Gene position: 2074601-2073378 (Counterclockwise)

Preceding gene: 238024994

Following gene: 238024992

Centisome position: 73.38

GC content: 69.85

Gene sequence:

>1224_bases
ATGCTGAAACGCCTGTTTGCGCGCGATTGCGCGCTCCGCTTCGCCCCCCGCGCCGCACCCCGGGCGGCCCGCCGGGCCGC
GCCCGCCATGCTGGCGATGCTGCTGGCCGCCTGCTCGAGCGCGCCCGGCAACTACCTCGACACCTCGCGCCTCGAGGATC
ACGATCCGACGCCGGACAAGCACTACAACGTGAAGCTGATCACGCCGCAGCTGGTGGTGGCGCAGGCGCTCGCGCAGCGC
AAGGAGCGGCCGCTGCCGCCCGACATGTTCAGCGATCCGAACCAGTACGTCTACCGGATCGAGCCGCAGGACATTCTCGG
CGTGACGGTCTGGGACCATCCCGAGCTGACCACCCCGCAGGGCCAGACGTTCTCGGCGGGCGGCAACACCACCCAGACCA
TCGCGGGCGCGCTGCAGCAGCCGTACACCAACGCGCTGCCGGGCCAGGCCGACCCCTACGGCCAGACCGTGGGCGCCGAC
GGCACCATCTACTTCCCGTTCGTCGGCCGCGTGCGTGCCGCGGGGCGCACCGCCCAGCAGCTGCGCGACGAGCTGACCGC
GCGGCTCGCCAGGTACGTGCGCGATCCGCAGATCGACGTGCGCGTGCTGTCGTACCGCAGCCAGAAGGTGCAGGTCACGG
GCGAGGTCAAGACCCCCGGGCCGCTCGCCATCACCGACGTGCCGCTGCGCCTGGTGGATGCGATCACGCGCTCGGGCGGC
TCGACCGCCGAGGCCGACTTGCAGCGCGTGCGGCTCACGCGCGATGGCAAGTTCTACGTGCTCGACGCGAATGCCATGCT
CGATCGCGGCGACGCGGCGCAGAACGTGATGCTGCAGCCGGGCGACGTGATCAACGTGCCGGACCGCAGCGACAGCCGCG
TCTTCGTGATGGGCGAGGTGAAGACGCCCGCCACGGTGCCGATGCTCAAGGGGCGCCTGACCATCGCCGACGCGCTGACG
GCCGGCGGCGGGATTCTCGATACCGATGCCAACCCGCGCCAGGTCCTGGTGCTGCGCGACATGCAGGACAAGCCGGACTC
GCCCGACATCTACCGGCTCGACATGACCCAGCCCGACGCGCTGATGCTGTCGAGCCGCTTCCAGCTCAAGCCGCTCGACG
TGGTCTACGTGGGCACGGCGGCCTCGGTCGAGTTCAACCGCCTGTTGCAGCAGATTTTCCCGACGATCCAGTCGGTCTAC
TACATGAAGCAGATCACGCGCTGA

Upstream 100 bases:

>100_bases
AGGACGCGGAAATTTCCGATCCGCACGGCGGCAGCGAATCCGCCTACCGCGAAAGCTGCCGGTTGATCGAACGTCTGGTC
CAGGGATGGCTGCAAAAACT

Downstream 100 bases:

>100_bases
TCTGACCGTGCCGGCATCATCCCCGATGCCGGCGACCGAACCCGGAGCGGACAAGCCATCGTGAATACGCAAGTCAAACA
CCCTTACGCGGACCTCGCCG

Product: Capsular polysaccharide biosynthesis/export periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLKRLFARDCALRFAPRAAPRAARRAAPAMLAMLLAACSSAPGNYLDTSRLEDHDPTPDKHYNVKLITPQLVVAQALAQR
KERPLPPDMFSDPNQYVYRIEPQDILGVTVWDHPELTTPQGQTFSAGGNTTQTIAGALQQPYTNALPGQADPYGQTVGAD
GTIYFPFVGRVRAAGRTAQQLRDELTARLARYVRDPQIDVRVLSYRSQKVQVTGEVKTPGPLAITDVPLRLVDAITRSGG
STAEADLQRVRLTRDGKFYVLDANAMLDRGDAAQNVMLQPGDVINVPDRSDSRVFVMGEVKTPATVPMLKGRLTIADALT
AGGGILDTDANPRQVLVLRDMQDKPDSPDIYRLDMTQPDALMLSSRFQLKPLDVVYVGTAASVEFNRLLQQIFPTIQSVY
YMKQITR

Sequences:

>Translated_407_residues
MLKRLFARDCALRFAPRAAPRAARRAAPAMLAMLLAACSSAPGNYLDTSRLEDHDPTPDKHYNVKLITPQLVVAQALAQR
KERPLPPDMFSDPNQYVYRIEPQDILGVTVWDHPELTTPQGQTFSAGGNTTQTIAGALQQPYTNALPGQADPYGQTVGAD
GTIYFPFVGRVRAAGRTAQQLRDELTARLARYVRDPQIDVRVLSYRSQKVQVTGEVKTPGPLAITDVPLRLVDAITRSGG
STAEADLQRVRLTRDGKFYVLDANAMLDRGDAAQNVMLQPGDVINVPDRSDSRVFVMGEVKTPATVPMLKGRLTIADALT
AGGGILDTDANPRQVLVLRDMQDKPDSPDIYRLDMTQPDALMLSSRFQLKPLDVVYVGTAASVEFNRLLQQIFPTIQSVY
YMKQITR
>Mature_407_residues
MLKRLFARDCALRFAPRAAPRAARRAAPAMLAMLLAACSSAPGNYLDTSRLEDHDPTPDKHYNVKLITPQLVVAQALAQR
KERPLPPDMFSDPNQYVYRIEPQDILGVTVWDHPELTTPQGQTFSAGGNTTQTIAGALQQPYTNALPGQADPYGQTVGAD
GTIYFPFVGRVRAAGRTAQQLRDELTARLARYVRDPQIDVRVLSYRSQKVQVTGEVKTPGPLAITDVPLRLVDAITRSGG
STAEADLQRVRLTRDGKFYVLDANAMLDRGDAAQNVMLQPGDVINVPDRSDSRVFVMGEVKTPATVPMLKGRLTIADALT
AGGGILDTDANPRQVLVLRDMQDKPDSPDIYRLDMTQPDALMLSSRFQLKPLDVVYVGTAASVEFNRLLQQIFPTIQSVY
YMKQITR

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1787218, Length=389, Percent_Identity=38.0462724935733, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1788376, Length=398, Percent_Identity=36.4321608040201, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 44805; Mature: 44805

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLFARDCALRFAPRAAPRAARRAAPAMLAMLLAACSSAPGNYLDTSRLEDHDPTPDK
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
HYNVKLITPQLVVAQALAQRKERPLPPDMFSDPNQYVYRIEPQDILGVTVWDHPELTTPQ
CEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
GQTFSAGGNTTQTIAGALQQPYTNALPGQADPYGQTVGADGTIYFPFVGRVRAAGRTAQQ
CCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCHHHHH
LRDELTARLARYVRDPQIDVRVLSYRSQKVQVTGEVKTPGPLAITDVPLRLVDAITRSGG
HHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCEEEECCHHHHHHHHHHCCC
STAEADLQRVRLTRDGKFYVLDANAMLDRGDAAQNVMLQPGDVINVPDRSDSRVFVMGEV
CCHHHHHHHHEEECCCEEEEEECCHHHCCCCCCCCEEECCCCEEECCCCCCCEEEEEECC
KTPATVPMLKGRLTIADALTAGGGILDTDANPRQVLVLRDMQDKPDSPDIYRLDMTQPDA
CCCCCCHHHCCCEEEEHHHHCCCCEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCCCH
LMLSSRFQLKPLDVVYVGTAASVEFNRLLQQIFPTIQSVYYMKQITR
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRLFARDCALRFAPRAAPRAARRAAPAMLAMLLAACSSAPGNYLDTSRLEDHDPTPDK
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
HYNVKLITPQLVVAQALAQRKERPLPPDMFSDPNQYVYRIEPQDILGVTVWDHPELTTPQ
CEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
GQTFSAGGNTTQTIAGALQQPYTNALPGQADPYGQTVGADGTIYFPFVGRVRAAGRTAQQ
CCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCHHHHH
LRDELTARLARYVRDPQIDVRVLSYRSQKVQVTGEVKTPGPLAITDVPLRLVDAITRSGG
HHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCEEEECCHHHHHHHHHHCCC
STAEADLQRVRLTRDGKFYVLDANAMLDRGDAAQNVMLQPGDVINVPDRSDSRVFVMGEV
CCHHHHHHHHEEECCCEEEEEECCHHHCCCCCCCCEEECCCCEEECCCCCCCEEEEEECC
KTPATVPMLKGRLTIADALTAGGGILDTDANPRQVLVLRDMQDKPDSPDIYRLDMTQPDA
CCCCCCHHHCCCEEEEHHHHCCCCEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCCCH
LMLSSRFQLKPLDVVYVGTAASVEFNRLLQQIFPTIQSVYYMKQITR
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]