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The map label for this gene is sfiIM [H]

Identifier: 238024955

GI number: 238024955

Start: 2032497

End: 2033639

Strand: Direct

Name: sfiIM [H]

Synonym: bglu_2g16100

Alternate gene names: 238024955

Gene position: 2032497-2033639 (Clockwise)

Preceding gene: 238024954

Following gene: 238024956

Centisome position: 71.89

GC content: 52.93

Gene sequence:

>1143_bases
GTGAGTGATGGAATCATTGAGGCGGTGAGCGGCGTATCGAACCGCAAGCAGAAAGTAAGCGATCGCCGCATGGTATCGCA
CGATATCGCGTCAAAAGACTTTGGCGAGGCTTTCGCTTCCTACGAACAGGAGGTGACCACTAATCCGTTATTGCTCAACG
GTGATGCGCTCAACGTGCTCCAAGCGCTTCCTGACGAATGCATCGACTGTGCGATGACCTCCCCGCCTTACTGGGGTAAG
CGTGAATATGAGAACGGCGGCATCGGTTTGGAAGCAGACTACCGCGACTTCGTCCGCGACCTCGCTGCAATATTCCTTGA
GCTCAAAAGGGTTCTAAAACCGACTGGATCATTCTGGCTCAACCTCGGCGATACATACGATGGCAAAGGACTTCTCGGTA
TTCCTTGGCGGGTTGCCTTCGAGCTAACAGACAACCAAGGTTGGACTATGCGCAACAGCGTAGTCTGGAACAAGTTGAAG
GGAGGCATGGACAACAGCAAGGACCGCCTAGCTAACGTTCACGAAAACCTATTTCACTTTGTGAAGAAAGCTAAGGGCTA
TTACTACAACGCTGACGCAATCCGTTCGAAGCCACGCGAGGCGAAAGTTGTGAATGGCGCCGTCATCTCCGCCACCGGGG
TTTCTGGAGTTCGGTACAAGCGACAGATTGAATTGTCTACGGCCCTTGACGACAATGAGAAGAAGGCTGCTTTCGCCGCA
CTTGATTCGATGCTTGCCGATCTTGCGGCGGGCAATGTCTCAGACTTCCGAATGATCATTCGCGGTCAACAGCGTGCGAC
CCATTCTGATAGCGAAAAGGTCTCGGGGCGAGCTAAGGAACTCCGGGATAAGGGCTTCTACTTTCTCAAATACCATCCCA
ATGGCAGTAAGCCGGGCGACGTGTGGGACATAATTCCCGAGGATACGCAACGGCGCGGCACACATTTTGCTCCTTATCCT
GTGGACCTTTGCCGTAGACCGTTACTCGCAACATGCCCACCGCGAGGCGTAGTTCTTGATCCCTTCTGCGGAACTGGGAC
AACCCTTCTCGCCGCACGTCAGATTGGCCTTAAGTCAATCGGAATCGATATTTCGCGCCAATACCTCGAACTATCACAAG
AAAGATGCAACAGCCTTCTATGA

Upstream 100 bases:

>100_bases
AATCAGTGACAGGTGGTCGCCCGAGCGCTCAAATTTAAACGCTTCTACTTTACTTACAACTCTCGGCTTAACTGGCCGCA
CGCAAAGTCAGGAATTTTTA

Downstream 100 bases:

>100_bases
GCAAAGTTGTTGAGTTATTCGGCAAAGCAGTGGACGCTCCGGGAATTGAGTGGCAAAAGACAATTGCCGAGCAGGGTTGC
CCATTCTTGGCCAAGCGATG

Product: DNA methyltransferase

Products: NA

Alternate protein names: M.SfiI; N-4 cytosine-specific methyltransferase SfiI [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSDGIIEAVSGVSNRKQKVSDRRMVSHDIASKDFGEAFASYEQEVTTNPLLLNGDALNVLQALPDECIDCAMTSPPYWGK
REYENGGIGLEADYRDFVRDLAAIFLELKRVLKPTGSFWLNLGDTYDGKGLLGIPWRVAFELTDNQGWTMRNSVVWNKLK
GGMDNSKDRLANVHENLFHFVKKAKGYYYNADAIRSKPREAKVVNGAVISATGVSGVRYKRQIELSTALDDNEKKAAFAA
LDSMLADLAAGNVSDFRMIIRGQQRATHSDSEKVSGRAKELRDKGFYFLKYHPNGSKPGDVWDIIPEDTQRRGTHFAPYP
VDLCRRPLLATCPPRGVVLDPFCGTGTTLLAARQIGLKSIGIDISRQYLELSQERCNSLL

Sequences:

>Translated_380_residues
MSDGIIEAVSGVSNRKQKVSDRRMVSHDIASKDFGEAFASYEQEVTTNPLLLNGDALNVLQALPDECIDCAMTSPPYWGK
REYENGGIGLEADYRDFVRDLAAIFLELKRVLKPTGSFWLNLGDTYDGKGLLGIPWRVAFELTDNQGWTMRNSVVWNKLK
GGMDNSKDRLANVHENLFHFVKKAKGYYYNADAIRSKPREAKVVNGAVISATGVSGVRYKRQIELSTALDDNEKKAAFAA
LDSMLADLAAGNVSDFRMIIRGQQRATHSDSEKVSGRAKELRDKGFYFLKYHPNGSKPGDVWDIIPEDTQRRGTHFAPYP
VDLCRRPLLATCPPRGVVLDPFCGTGTTLLAARQIGLKSIGIDISRQYLELSQERCNSLL
>Mature_379_residues
SDGIIEAVSGVSNRKQKVSDRRMVSHDIASKDFGEAFASYEQEVTTNPLLLNGDALNVLQALPDECIDCAMTSPPYWGKR
EYENGGIGLEADYRDFVRDLAAIFLELKRVLKPTGSFWLNLGDTYDGKGLLGIPWRVAFELTDNQGWTMRNSVVWNKLKG
GMDNSKDRLANVHENLFHFVKKAKGYYYNADAIRSKPREAKVVNGAVISATGVSGVRYKRQIELSTALDDNEKKAAFAAL
DSMLADLAAGNVSDFRMIIRGQQRATHSDSEKVSGRAKELRDKGFYFLKYHPNGSKPGDVWDIIPEDTQRRGTHFAPYPV
DLCRRPLLATCPPRGVVLDPFCGTGTTLLAARQIGLKSIGIDISRQYLELSQERCNSLL

Specific function: This methylase recognizes the double-stranded sequence GGCCNNNNNGGCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the SfiI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 42276; Mature: 42145

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGIIEAVSGVSNRKQKVSDRRMVSHDIASKDFGEAFASYEQEVTTNPLLLNGDALNVL
CCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCHHHHHH
QALPDECIDCAMTSPPYWGKREYENGGIGLEADYRDFVRDLAAIFLELKRVLKPTGSFWL
HHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
NLGDTYDGKGLLGIPWRVAFELTDNQGWTMRNSVVWNKLKGGMDNSKDRLANVHENLFHF
ECCCCCCCCEEEECCEEEEEEEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VKKAKGYYYNADAIRSKPREAKVVNGAVISATGVSGVRYKRQIELSTALDDNEKKAAFAA
HHHHCCCEECCHHHHCCCCHHEEECCEEEEECCCCCCEEEEEEEEHHCCCCCHHHHHHHH
LDSMLADLAAGNVSDFRMIIRGQQRATHSDSEKVSGRAKELRDKGFYFLKYHPNGSKPGD
HHHHHHHHHCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
VWDIIPEDTQRRGTHFAPYPVDLCRRPLLATCPPRGVVLDPFCGTGTTLLAARQIGLKSI
EEECCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCHHHH
GIDISRQYLELSQERCNSLL
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDGIIEAVSGVSNRKQKVSDRRMVSHDIASKDFGEAFASYEQEVTTNPLLLNGDALNVL
CCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCHHHHHH
QALPDECIDCAMTSPPYWGKREYENGGIGLEADYRDFVRDLAAIFLELKRVLKPTGSFWL
HHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
NLGDTYDGKGLLGIPWRVAFELTDNQGWTMRNSVVWNKLKGGMDNSKDRLANVHENLFHF
ECCCCCCCCEEEECCEEEEEEEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VKKAKGYYYNADAIRSKPREAKVVNGAVISATGVSGVRYKRQIELSTALDDNEKKAAFAA
HHHHCCCEECCHHHHCCCCHHEEECCEEEEECCCCCCEEEEEEEEHHCCCCCHHHHHHHH
LDSMLADLAAGNVSDFRMIIRGQQRATHSDSEKVSGRAKELRDKGFYFLKYHPNGSKPGD
HHHHHHHHHCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
VWDIIPEDTQRRGTHFAPYPVDLCRRPLLATCPPRGVVLDPFCGTGTTLLAARQIGLKSI
EEECCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCHHHH
GIDISRQYLELSQERCNSLL
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA