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The map label for this gene is acnB [H]

Identifier: 238024834

GI number: 238024834

Start: 1872042

End: 1874627

Strand: Reverse

Name: acnB [H]

Synonym: bglu_2g14740

Alternate gene names: 238024834

Gene position: 1874627-1872042 (Counterclockwise)

Preceding gene: 238024835

Following gene: 238024833

Centisome position: 66.3

GC content: 67.01

Gene sequence:

>2586_bases
ATGCTTGAAAACTTTCGTGCTCACGTGGCCGCCCGCGCCGCGCTTGGTATCCCGCCCCTGCCGTTGACGGCCCAACAGAC
CGCCGAGTTGGTGGAGCTGCTGGTGAATCCGCCCGCCGGCGAAGAGCAGACGCTGCTCGACCTGATCACCCACCGCGTGC
CCGCCGGCGTCGACGAAGCCGCGCGCGTGAAGGCCGGCTTCCTGGCCGCCGTGGCCAAGGGCGAGACCGCCTGCGCGCTG
ATCTCGCGCGAGCGCGCGACCGAGCTGCTCGGCACCATGCTGGGCGGCTACAACATCCAGCCGCTGATCGAACTGCTGTC
CGACGAGGCGGTGGCCGCCGTGGCCGCCGACGCGCTCAAGAAGACGCTGCTGATGTTCGACCAGTTCCACGACGTCAAGG
AACTGGCCGAGCAGGGCAACGCGCACGCCAAGGCCGTGCTGCAGAGCTGGGCCGACGCCGAGTGGTTCACGAGCCGCCCG
GAAGTGCCGCAGAGCCTGACCGTCACCGTCTTCAAGGTGCCGGGCGAAACCAATACCGACGATCTCTCGCCGGCCCCGGA
CGCCACCACGCGCCCCGACATCCCGCTGCACGCGCTGGCGATGCTGAAGAACGCGCGCCCCGGCATCACCCCGGAAGAGG
ACGGCAAGCGCGGCCCGGTGAAGTTCATCGAGTCGCTGAAGGAGAAGGGCCATCTGGTCGCCTACGTGGGCGACGTGGTC
GGCACCGGCTCCTCGCGCAAGTCGGCCACCAATTCGGTGCTTTGGTTCACGGGCGAGGACATCCCGTTCGTGCCGAACAA
GCGCTTCGGCGGCGTGTGCCTGGGCGGCAAGATCGCGCCGATCTTCTACAACACGATGGAAGATGCCGGCGCGCTGCCGA
TCGAACTCGACGTCTCGCAGATGGAAATGGGCGACGTGATCGAACTGCGTCCCTATGAAGGCAAGGCACTCAAGAACGGC
GAGGTGATCGCCGAGTTCCAGGTGAAGTCCGACGTGCTGTTCGACGAAGTGCGCGCCGGCGGCCGGATCCCGCTCATCAT
CGGCCGCGGCTTGACCGCCAAGGCGCGCGAGGCGCTGGGGCTGGCCCCGTCGACGCTGTTCCGTCTGCCGCATCAGCCGG
CCGACAGCGGCCGCGGCTTCTCGCTCGCGCAGAAGATGGTCGGCCGCGCCTGCGGCCTGCCGGAAGGCCAGGGCGTGCGC
CCGGGCACCTACTGCGAGCCGAAGATGACCTCGGTGGGTTCGCAGGACACCACCGGCCCGATGACGCGCGACGAGCTGAA
GGATCTGGCCTGCCTCGGCTTTTCTGCCGACCTCGTGATGCAGTCGTTCTGCCACACGGCGGCCTATCCGAAGCCGGTGG
ACGTGAAAACGCACCAGACGCTGCCGAACTTCATCAGCACGCGCGGCGGCATCGCGCTGCGCCCCGGCGACGGCGTGATC
CACTCCTGGCTGAACCGCATGCTGCTGCCCGACACGGTCGGCACCGGCGGCGATTCGCACACGCGTTTCCCGATCGGCAT
CAGCTTCCCGGCCGGCTCGGGCCTGGTCGCGTTCGCCGCCGCCACCGGCACGATGCCGCTCGACATGCCGGAATCGGTGC
TGGTCCGCTTCAAGGGCAAGATGCAGCCGGGCGTCACGCTGCGCGACCTCGTCAACGCGATTCCGCTCTATGCGATCAAG
CAGGGCATGCTGACGGTGGCCAAGCAGGGCAAGAAAAACATCTTCTCGGGCCGCATCCTCGAGATCGAGGGCCTGCCGGA
CCTGAAGGTCGAGCAGGCGTTCGAGCTGTCGGACGCGTCGGCCGAGCGTTCGGCCGCCGGCTGCACGGTGCGGCTGAACA
AGGAGCCGATCATCGAGTACCTCAACAGCAACGTCACGCTGCTGAAGTGGATGATCGCCGAGGGCTACCAGGACCCGCGC
AGCCTGCAGCGCCGCATCGCGGCGATGGAGCAGTGGCTGGCCGACCCGCAGTTGCTGGAGCCGGATGCCGACGCCGACTA
CGCCGCCGTGATCGAGATCGATCTGGCCGACATCCACGAGCCGATCGTGGCCTGCCCGAACGATCCGGACGACGTGAAGA
CGCTGTCGGACGTGGCCGGCGCCAAGATCGACGAAGTGTTCATCGGCTCGTGCATGACCAACATCGGCCACTTCCGCGCC
GCCTCGAAGCTGCTGGAAGGCAAGCGCGACATTCCCGTCAAGCTGTGGGTCGCGCCGCCGACCAAGATGGACCAGAAGCA
GCTGACCGAGGAAGGCCATTACGGCGTGTTCGGCACGGCCGGCGCGCGCACCGAAATGCCGGGCTGCTCGCTGTGCATGG
GCAACCAGGCACAGGTGCGCGAAGGCGCGACGGTGATGTCGACCTCCACGCGCAACTTCCCGAACCGTCTCGGCAAGAAC
ACCAACGTGTATCTCGGCTCGGCGGAACTGGCGGCGATCTGCTCGCGTCTGGGCAAGATCCCGACCAGGGAAGAGTACAT
GGCCGACATGGGCGTGCTGACCGCCAATGGCGACAAGATCTACCAGTACATGAACTTCGACCAGATCGCGGATTTCAAGG
AAGTGGCGGATACGGTCAAGGCGTAA

Upstream 100 bases:

>100_bases
TTTGATCCAATCCGATTAGAATCGTGGCCGAAGCAGCGCCCGTACGCATTCCGGAGCGTCTGACGCGCCATCCCCTGAAC
GCCATACCAGCAGGTTCCCC

Downstream 100 bases:

>100_bases
GCAGCCTGAGGATCGGGCCACGCGCGAGGGATGCGGCGTGGCCTCCGAGAAGCGCCGCGGGGGAATACCCGCGGCGCTTT
TTTTGTCGGTGCGGGCGCGG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLVNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACAL
ISRERATELLGTMLGGYNIQPLIELLSDEAVAAVAADALKKTLLMFDQFHDVKELAEQGNAHAKAVLQSWADAEWFTSRP
EVPQSLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSQMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLAPSTLFRLPHQPADSGRGFSLAQKMVGRACGLPEGQGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNVTLLKWMIAEGYQDPR
SLQRRIAAMEQWLADPQLLEPDADADYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGKIPTREEYMADMGVLTANGDKIYQYMNFDQIADFKEVADTVKA

Sequences:

>Translated_861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLVNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACAL
ISRERATELLGTMLGGYNIQPLIELLSDEAVAAVAADALKKTLLMFDQFHDVKELAEQGNAHAKAVLQSWADAEWFTSRP
EVPQSLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSQMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLAPSTLFRLPHQPADSGRGFSLAQKMVGRACGLPEGQGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNVTLLKWMIAEGYQDPR
SLQRRIAAMEQWLADPQLLEPDADADYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGKIPTREEYMADMGVLTANGDKIYQYMNFDQIADFKEVADTVKA
>Mature_861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLVNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACAL
ISRERATELLGTMLGGYNIQPLIELLSDEAVAAVAADALKKTLLMFDQFHDVKELAEQGNAHAKAVLQSWADAEWFTSRP
EVPQSLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSQMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLAPSTLFRLPHQPADSGRGFSLAQKMVGRACGLPEGQGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNVTLLKWMIAEGYQDPR
SLQRRIAAMEQWLADPQLLEPDADADYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGKIPTREEYMADMGVLTANGDKIYQYMNFDQIADFKEVADTVKA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=74.3619489559165, Blast_Score=1280, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=375, Percent_Identity=25.0666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=360, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92696; Mature: 92696

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLVNPPAGEEQTLLDLITHRVPAGVDEA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKAGFLAAVAKGETACALISRERATELLGTMLGGYNIQPLIELLSDEAVAAVAADALK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
KTLLMFDQFHDVKELAEQGNAHAKAVLQSWADAEWFTSRPEVPQSLTVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSQ
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHHCCCCEEEEEHHH
MEMGDVIELRPYEGKALKNGEVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALG
HCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
LAPSTLFRLPHQPADSGRGFSLAQKMVGRACGLPEGQGVRPGTYCEPKMTSVGSQDTTGP
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
MTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGIALRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHCCCC
MQPGVTLRDLVNAIPLYAIKQGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
AERSAAGCTVRLNKEPIIEYLNSNVTLLKWMIAEGYQDPRSLQRRIAAMEQWLADPQLLE
CHHCCCCCEEEECCHHHHHHHCCCHHEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
PDADADYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPTREEYMADMGVLTANGDKI
CCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCCEEECCCHHE
YQYMNFDQIADFKEVADTVKA
EECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLVNPPAGEEQTLLDLITHRVPAGVDEA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKAGFLAAVAKGETACALISRERATELLGTMLGGYNIQPLIELLSDEAVAAVAADALK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
KTLLMFDQFHDVKELAEQGNAHAKAVLQSWADAEWFTSRPEVPQSLTVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSQ
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHHCCCCEEEEEHHH
MEMGDVIELRPYEGKALKNGEVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALG
HCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
LAPSTLFRLPHQPADSGRGFSLAQKMVGRACGLPEGQGVRPGTYCEPKMTSVGSQDTTGP
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
MTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGIALRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHCCCC
MQPGVTLRDLVNAIPLYAIKQGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
AERSAAGCTVRLNKEPIIEYLNSNVTLLKWMIAEGYQDPRSLQRRIAAMEQWLADPQLLE
CHHCCCCCEEEECCHHHHHHHCCCHHEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
PDADADYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGKIPTREEYMADMGVLTANGDKI
CCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCCEEECCCHHE
YQYMNFDQIADFKEVADTVKA
EECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]