Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 229918402

GI number: 229918402

Start: 2653208

End: 2654338

Strand: Reverse

Name: nifS [H]

Synonym: EAT1b_2687

Alternate gene names: 229918402

Gene position: 2654338-2653208 (Counterclockwise)

Preceding gene: 229918411

Following gene: 229918401

Centisome position: 88.48

GC content: 51.55

Gene sequence:

>1131_bases
ATGAACCGCTACTTCGACTACGCCGCTACACACCCGATGACGCATGCAGCATTAGACCATTACGTGGCGATGGCCAAAAA
CGTTTATGGGAACCCATCATCACTCCATACCCAAGGTTATATGGCAGAAGATTATTTGACAGAGGCACGACGCTTGATCA
AACAGGCGCTCGGGCTTGATATGTTGCGAGGAAAATTATTATTCACCGGGAGTGGATCGGAGAGCAATTACATCGCCCTG
ACAAGTTTACGTAAACGGATGAAGGGCAAAGAGGTGATCACGTCTTGGCATGAACATGATTCGGTCGCTCTCTTACTTGA
GGAGTGGGAACGGGAAGGAATAATCGTTCATCGGTTGAAACTGAAGGATGGGAAGTTTGATTTAGATGCATTCACCCACC
TACTCGACCGTGACATCGGGCTCGTCACGCTCCAGCACGTCAATTCCGACACGGGTTGGGTGTTACCGCTCCATCACATC
CAATGCCTCTTGAAATATAAGAAAATCCTCTTTCATGTGGACGGGGTCCAGGCACTCGGGAAAATTCCGATTTCTGTCGA
AGGCATCGATGCCTATTCGTTCAGTGCCCACAAAGTGGGTGGACCGAAAGGAGTCGGCGCCCTCTATATGGAACGTCTGA
TTCCTCCCCCATACTATCCGGGACATCATCAATACGGGATTCGGCCCGGAACGATTGACGTTCCTGGAATCAGTGCCTTT
GCCAAAGCACTCGGAGAACGTCAGGAACAATTGACACGTTTTCAGAGAAATTTCATGGCGTTTCGTGCTATCTTGAAAGA
GAAGACGTCAGCGCATGTCCATTGGCTCGAGTTCGACGAACAAAGTCCCGCCATTTTCTCCTTCGTCTCCCATCAACGCG
ACGGACAATGGTGGATGACCGAGCTCGACGACCTCGGGTTCCAAGTATCGGCTGGGTCGGCATGTCGAGCAGGCCAAAAT
GGACCGAACCGCATGCTCGAGGCACTCGGATATGAGACGAGCGCCTGTCATGGACTCGTCCGCATTTCCTTCGGCGCGTA
TACGACCGCCGAAGACACATCGGCCCTCGCCGATGCCATCGTCATTTTAGCAAGGAGAGTCGAACATCATGAACAAACGT
TTAGCCGGTGA

Upstream 100 bases:

>100_bases
GACACTTGTACATCACCTGTTTTTCATATGTAATGTTGACTTGTAAACTAGTTTCGCATAATTTTGTGTAGAAGACAACA
TGAGAAAAGGGGAGTCCATG

Downstream 100 bases:

>100_bases
AGAACGCCGTCGCACACTATTACAATTGATTGAATCGAGCAATGAGCCAATCACAGGATCTGAACTGGCAAGACATGTAT
CCGTCAGTCGCCAGGTCATC

Product: aminotransferase class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNRYFDYAATHPMTHAALDHYVAMAKNVYGNPSSLHTQGYMAEDYLTEARRLIKQALGLDMLRGKLLFTGSGSESNYIAL
TSLRKRMKGKEVITSWHEHDSVALLLEEWEREGIIVHRLKLKDGKFDLDAFTHLLDRDIGLVTLQHVNSDTGWVLPLHHI
QCLLKYKKILFHVDGVQALGKIPISVEGIDAYSFSAHKVGGPKGVGALYMERLIPPPYYPGHHQYGIRPGTIDVPGISAF
AKALGERQEQLTRFQRNFMAFRAILKEKTSAHVHWLEFDEQSPAIFSFVSHQRDGQWWMTELDDLGFQVSAGSACRAGQN
GPNRMLEALGYETSACHGLVRISFGAYTTAEDTSALADAIVILARRVEHHEQTFSR

Sequences:

>Translated_376_residues
MNRYFDYAATHPMTHAALDHYVAMAKNVYGNPSSLHTQGYMAEDYLTEARRLIKQALGLDMLRGKLLFTGSGSESNYIAL
TSLRKRMKGKEVITSWHEHDSVALLLEEWEREGIIVHRLKLKDGKFDLDAFTHLLDRDIGLVTLQHVNSDTGWVLPLHHI
QCLLKYKKILFHVDGVQALGKIPISVEGIDAYSFSAHKVGGPKGVGALYMERLIPPPYYPGHHQYGIRPGTIDVPGISAF
AKALGERQEQLTRFQRNFMAFRAILKEKTSAHVHWLEFDEQSPAIFSFVSHQRDGQWWMTELDDLGFQVSAGSACRAGQN
GPNRMLEALGYETSACHGLVRISFGAYTTAEDTSALADAIVILARRVEHHEQTFSR
>Mature_376_residues
MNRYFDYAATHPMTHAALDHYVAMAKNVYGNPSSLHTQGYMAEDYLTEARRLIKQALGLDMLRGKLLFTGSGSESNYIAL
TSLRKRMKGKEVITSWHEHDSVALLLEEWEREGIIVHRLKLKDGKFDLDAFTHLLDRDIGLVTLQHVNSDTGWVLPLHHI
QCLLKYKKILFHVDGVQALGKIPISVEGIDAYSFSAHKVGGPKGVGALYMERLIPPPYYPGHHQYGIRPGTIDVPGISAF
AKALGERQEQLTRFQRNFMAFRAILKEKTSAHVHWLEFDEQSPAIFSFVSHQRDGQWWMTELDDLGFQVSAGSACRAGQN
GPNRMLEALGYETSACHGLVRISFGAYTTAEDTSALADAIVILARRVEHHEQTFSR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=378, Percent_Identity=28.8359788359788, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI156713448, Length=399, Percent_Identity=26.5664160401003, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI48994898, Length=366, Percent_Identity=31.1475409836066, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25143064, Length=381, Percent_Identity=29.1338582677165, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=28.7925696594427, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=28.6486486486486, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI20129463, Length=378, Percent_Identity=30.952380952381, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42304; Mature: 42304

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYFDYAATHPMTHAALDHYVAMAKNVYGNPSSLHTQGYMAEDYLTEARRLIKQALGLD
CCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHH
MLRGKLLFTGSGSESNYIALTSLRKRMKGKEVITSWHEHDSVALLLEEWEREGIIVHRLK
HHCCEEEEEECCCCCCEEEHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
LKDGKFDLDAFTHLLDRDIGLVTLQHVNSDTGWVLPLHHIQCLLKYKKILFHVDGVQALG
ECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHC
KIPISVEGIDAYSFSAHKVGGPKGVGALYMERLIPPPYYPGHHQYGIRPGTIDVPGISAF
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AKALGERQEQLTRFQRNFMAFRAILKEKTSAHVHWLEFDEQSPAIFSFVSHQRDGQWWMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHH
ELDDLGFQVSAGSACRAGQNGPNRMLEALGYETSACHGLVRISFGAYTTAEDTSALADAI
HHHHCCEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCEEEEEEECCCCCCCHHHHHHHHH
VILARRVEHHEQTFSR
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRYFDYAATHPMTHAALDHYVAMAKNVYGNPSSLHTQGYMAEDYLTEARRLIKQALGLD
CCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHH
MLRGKLLFTGSGSESNYIALTSLRKRMKGKEVITSWHEHDSVALLLEEWEREGIIVHRLK
HHCCEEEEEECCCCCCEEEHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
LKDGKFDLDAFTHLLDRDIGLVTLQHVNSDTGWVLPLHHIQCLLKYKKILFHVDGVQALG
ECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHC
KIPISVEGIDAYSFSAHKVGGPKGVGALYMERLIPPPYYPGHHQYGIRPGTIDVPGISAF
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AKALGERQEQLTRFQRNFMAFRAILKEKTSAHVHWLEFDEQSPAIFSFVSHQRDGQWWMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHH
ELDDLGFQVSAGSACRAGQNGPNRMLEALGYETSACHGLVRISFGAYTTAEDTSALADAI
HHHHCCEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCEEEEEEECCCCCCCHHHHHHHHH
VILARRVEHHEQTFSR
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]