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The map label for this gene is hchA [H]

Identifier: 229917816

GI number: 229917816

Start: 2047445

End: 2048107

Strand: Reverse

Name: hchA [H]

Synonym: EAT1b_2094

Alternate gene names: 229917816

Gene position: 2048107-2047445 (Counterclockwise)

Preceding gene: 229917817

Following gene: 229917815

Centisome position: 68.27

GC content: 49.32

Gene sequence:

>663_bases
ATGAGTAAACATGTATTAATGGTCGTGACGAACGCAAAAGAAATGAAGGACGGTCACACGACTGGACTTTGGTTGTCCGA
GTTCGGTGAGGCATACGTCGAGTTCAAACAACAAGGATATGACATCACGGTCGCTAGCCCGAACGGTGGTCAATCTCCAA
TCGACGCACGCAGTTTAGAAGGAGAAGTACCGGCTGACATTCAAGCGACGGCTCCCCTTCTCGAAGACACAATCGATTTG
AAGACGATCACAAACTTCGATCAGTTTGATGCCATCTTCATGCCAGGTGGTCACGGAACGATGTTCGACCTTCCACAAAG
CGAAGCGTTGAATGTTGCGATCCGTTCACTATTTGAAGCAGGTAAAACGGTTGCTGCCGTCTGCCACGGTCCAGCTGGTC
TAGTCGGCGCGACATTGTCTGACGGTACACCACTCGTAAACGGTAAAACGATTGCGACGTTCACGGACGAAGAAGAACGT
GCGACAGGTCTTGATATCTACATGCCGTTCATGCTTGAAACGCGTCTTCGTGAACTCGGTGCGAACATCGTGCTCGCAGA
CAACTTCACAGAAAACGTTCAAGTCGATGGGAACTTGGTAACAGGCCAAAATCCACAATCGACCGTTGCTGTCGCAAAAG
CCGTCATCAACACATTAAACTAA

Upstream 100 bases:

>100_bases
TTATAGATAAGGTGTTTTTTCTTAATTTGTTTTTCGTTAAAGTGCGCGTTATTCTAGTCACGTACCGAAACAACGGATGA
TTTCTTAGGAGGAATGACAG

Downstream 100 bases:

>100_bases
TCGATTCTTTCTCGTTGCCAATCGATGAAAGAGAAAGACCCGCCTGTTCTCGAACAGGCGGGTTTTATAGATGAGGAGGA
ATGAAGATGATGAACATCTT

Product: ThiJ/PfpI domain protein

Products: NA

Alternate protein names: Hsp31 [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSKHVLMVVTNAKEMKDGHTTGLWLSEFGEAYVEFKQQGYDITVASPNGGQSPIDARSLEGEVPADIQATAPLLEDTIDL
KTITNFDQFDAIFMPGGHGTMFDLPQSEALNVAIRSLFEAGKTVAAVCHGPAGLVGATLSDGTPLVNGKTIATFTDEEER
ATGLDIYMPFMLETRLRELGANIVLADNFTENVQVDGNLVTGQNPQSTVAVAKAVINTLN

Sequences:

>Translated_220_residues
MSKHVLMVVTNAKEMKDGHTTGLWLSEFGEAYVEFKQQGYDITVASPNGGQSPIDARSLEGEVPADIQATAPLLEDTIDL
KTITNFDQFDAIFMPGGHGTMFDLPQSEALNVAIRSLFEAGKTVAAVCHGPAGLVGATLSDGTPLVNGKTIATFTDEEER
ATGLDIYMPFMLETRLRELGANIVLADNFTENVQVDGNLVTGQNPQSTVAVAKAVINTLN
>Mature_219_residues
SKHVLMVVTNAKEMKDGHTTGLWLSEFGEAYVEFKQQGYDITVASPNGGQSPIDARSLEGEVPADIQATAPLLEDTIDLK
TITNFDQFDAIFMPGGHGTMFDLPQSEALNVAIRSLFEAGKTVAAVCHGPAGLVGATLSDGTPLVNGKTIATFTDEEERA
TGLDIYMPFMLETRLRELGANIVLADNFTENVQVDGNLVTGQNPQSTVAVAKAVINTLN

Specific function: Uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. It rapidly releases them once stress has abated [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C56 family. HchA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788278, Length=130, Percent_Identity=33.0769230769231, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320742, Length=233, Percent_Identity=29.6137339055794, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324967, Length=220, Percent_Identity=27.7272727272727, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324975, Length=220, Percent_Identity=27.7272727272727, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323984, Length=220, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017283
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 23488; Mature: 23357

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHVLMVVTNAKEMKDGHTTGLWLSEFGEAYVEFKQQGYDITVASPNGGQSPIDARSLE
CCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
GEVPADIQATAPLLEDTIDLKTITNFDQFDAIFMPGGHGTMFDLPQSEALNVAIRSLFEA
CCCCCCCCHHCHHHHHCCCCEEECCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHC
GKTVAAVCHGPAGLVGATLSDGTPLVNGKTIATFTDEEERATGLDIYMPFMLETRLRELG
CCEEEEEECCCCCEEEEECCCCCCEECCCEEEEECCCHHHHCCCEEEHHHHHHHHHHHCC
ANIVLADNFTENVQVDGNLVTGQNPQSTVAVAKAVINTLN
CCEEEECCCCCCEEECCEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKHVLMVVTNAKEMKDGHTTGLWLSEFGEAYVEFKQQGYDITVASPNGGQSPIDARSLE
CCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
GEVPADIQATAPLLEDTIDLKTITNFDQFDAIFMPGGHGTMFDLPQSEALNVAIRSLFEA
CCCCCCCCHHCHHHHHCCCCEEECCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHC
GKTVAAVCHGPAGLVGATLSDGTPLVNGKTIATFTDEEERATGLDIYMPFMLETRLRELG
CCEEEEEECCCCCEEEEECCCCCCEECCCEEEEECCCHHHHCCCEEEHHHHHHHHHHHCC
ANIVLADNFTENVQVDGNLVTGQNPQSTVAVAKAVINTLN
CCEEEECCCCCCEEECCEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA