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The map label for this gene is yidA [H]

Identifier: 229917734

GI number: 229917734

Start: 1964048

End: 1964869

Strand: Reverse

Name: yidA [H]

Synonym: EAT1b_2011

Alternate gene names: 229917734

Gene position: 1964869-1964048 (Counterclockwise)

Preceding gene: 229917739

Following gene: 229917733

Centisome position: 65.5

GC content: 50.24

Gene sequence:

>822_bases
ATGAGTTACAAGATGATTGTATTGGATATGGATGATACGCTCCTCACGAGCGACCATACGATTTCCCCTAAGACAAAAGA
AGCACTTCTCGATGCACAGGCGCGCGGCTATAAGGTCGTACTCGCGAGCGGACGACCGACGTTTGCGATGTGGGATGCAG
CCCGTGAATTAGAACTTGCGAAATACGGAAGCTACATCTTATCGTTCAACGGTGCCTCGGTCATCAATTGTGCGACAAAC
GAGGAGATTTTCTCGAGCACGCTCCAACCGGATACAGTCGCCCATCTCCATAATATCAGTGAACGGGAAGGAATCGCGAT
TCATACGTACGTCGGTAACGAAATCGTAACCGATCGCCCGAACCCTTATACGGATATCGAAGGTGAGTTGACAGGAATGC
CGGTCATTCATGTCGACAACTTTAAAGATGCCGTGACAGCCCCGGTCGTCAAATGTTTGATGCTTGCGGATGGTGACACA
CTCGCTCCGATCGAAACAAAGCTACAAGAAGAATTGGCTGGAAAATTGGCCGTGGCCCGTTCAAAACCGTTCTTCCTTGA
GTTTACGGAAGCGGGTGTCACAAAAGGAACGAGTCTCGCCTTCCTTGCAGACCAGCTCGGCATCGCGCAAAAAGAAGTCA
TCGCTTGTGGTGACGGAAACAATGACTTGACGATGATTGAATGGGCCGGCCTCGGCGTCGCGATGGGAAATGCCAACACG
ACGGTCAAAGGGAAAGCGGACTTCATCACAAAGTCAAACAACGAAGACGGAATCGCTCATGTCATCGAAACGTATATGAC
GGACGTACTCGTCACGCAATAA

Upstream 100 bases:

>100_bases
TCTCTACATTATAGTTACGTTTTTGCGACAAGGTTGTCAATTGACCTTAAAAAGGTTTAGGATGGAGTCATTAGATTCTG
AAGGAGGACCCCCTACTACT

Downstream 100 bases:

>100_bases
GCTTCTGATTGTAGAAAGGGAGGAAGGAAGATGAATAAAGGGTTGAAACGAGCTGGAGTCGGATTGATTGGAACTGCGGT
CGCAGGAGCGGCTTATGTGT

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSYKMIVLDMDDTLLTSDHTISPKTKEALLDAQARGYKVVLASGRPTFAMWDAARELELAKYGSYILSFNGASVINCATN
EEIFSSTLQPDTVAHLHNISEREGIAIHTYVGNEIVTDRPNPYTDIEGELTGMPVIHVDNFKDAVTAPVVKCLMLADGDT
LAPIETKLQEELAGKLAVARSKPFFLEFTEAGVTKGTSLAFLADQLGIAQKEVIACGDGNNDLTMIEWAGLGVAMGNANT
TVKGKADFITKSNNEDGIAHVIETYMTDVLVTQ

Sequences:

>Translated_273_residues
MSYKMIVLDMDDTLLTSDHTISPKTKEALLDAQARGYKVVLASGRPTFAMWDAARELELAKYGSYILSFNGASVINCATN
EEIFSSTLQPDTVAHLHNISEREGIAIHTYVGNEIVTDRPNPYTDIEGELTGMPVIHVDNFKDAVTAPVVKCLMLADGDT
LAPIETKLQEELAGKLAVARSKPFFLEFTEAGVTKGTSLAFLADQLGIAQKEVIACGDGNNDLTMIEWAGLGVAMGNANT
TVKGKADFITKSNNEDGIAHVIETYMTDVLVTQ
>Mature_272_residues
SYKMIVLDMDDTLLTSDHTISPKTKEALLDAQARGYKVVLASGRPTFAMWDAARELELAKYGSYILSFNGASVINCATNE
EIFSSTLQPDTVAHLHNISEREGIAIHTYVGNEIVTDRPNPYTDIEGELTGMPVIHVDNFKDAVTAPVVKCLMLADGDTL
APIETKLQEELAGKLAVARSKPFFLEFTEAGVTKGTSLAFLADQLGIAQKEVIACGDGNNDLTMIEWAGLGVAMGNANTT
VKGKADFITKSNNEDGIAHVIETYMTDVLVTQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=272, Percent_Identity=37.5, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI48994981, Length=245, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786982, Length=273, Percent_Identity=27.1062271062271, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081790, Length=272, Percent_Identity=26.1029411764706, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081741, Length=243, Percent_Identity=26.3374485596708, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29507; Mature: 29376

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKMIVLDMDDTLLTSDHTISPKTKEALLDAQARGYKVVLASGRPTFAMWDAARELELA
CCEEEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHH
KYGSYILSFNGASVINCATNEEIFSSTLQPDTVAHLHNISEREGIAIHTYVGNEIVTDRP
HCCCEEEEECCCEEEEECCCHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCEEECCCC
NPYTDIEGELTGMPVIHVDNFKDAVTAPVVKCLMLADGDTLAPIETKLQEELAGKLAVAR
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEECCCCEECHHHHHHHHHHHHHEEEEC
SKPFFLEFTEAGVTKGTSLAFLADQLGIAQKEVIACGDGNNDLTMIEWAGLGVAMGNANT
CCCEEEEEECCCCCCCCCCEEHHHHHCCCCEEEEEECCCCCCEEEEEEECCEEEECCCCC
TVKGKADFITKSNNEDGIAHVIETYMTDVLVTQ
EEECCEEEEECCCCCCHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SYKMIVLDMDDTLLTSDHTISPKTKEALLDAQARGYKVVLASGRPTFAMWDAARELELA
CEEEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHH
KYGSYILSFNGASVINCATNEEIFSSTLQPDTVAHLHNISEREGIAIHTYVGNEIVTDRP
HCCCEEEEECCCEEEEECCCHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCEEECCCC
NPYTDIEGELTGMPVIHVDNFKDAVTAPVVKCLMLADGDTLAPIETKLQEELAGKLAVAR
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEECCCCEECHHHHHHHHHHHHHEEEEC
SKPFFLEFTEAGVTKGTSLAFLADQLGIAQKEVIACGDGNNDLTMIEWAGLGVAMGNANT
CCCEEEEEECCCCCCCCCCEEHHHHHCCCCEEEEEECCCCCCEEEEEEECCEEEECCCCC
TVKGKADFITKSNNEDGIAHVIETYMTDVLVTQ
EEECCEEEEECCCCCCHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]