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The map label for this gene is dagK [H]

Identifier: 229917727

GI number: 229917727

Start: 1957529

End: 1958413

Strand: Reverse

Name: dagK [H]

Synonym: EAT1b_2004

Alternate gene names: 229917727

Gene position: 1958413-1957529 (Counterclockwise)

Preceding gene: 229917728

Following gene: 229917726

Centisome position: 65.28

GC content: 51.41

Gene sequence:

>885_bases
ATGGACACAGTCATGTTAATCGTCAATCCATCCTCAGGAAAAGAATTGGGGGAACAACACGCAACGCATGCCGAAGAGGT
GTTGCGCGAACGATATGGACACGTCGATGTCCGCTTCACTGAAAAGGAACAGGATGCGACGAACTTCGCACGTGAAGCGG
CACAAAAGCACTATCAGGCCGTCATTGCGATGGGTGGGGACGGAACCTTGAACGAAGCGGTGACCGGTCTAGCTGAGGCT
ACTTATCGACCTGACTTCGGCATCATTCCACTCGGGACGGTCAACGACCTCGCCCGTGCACTCGGTGTCCCAAGTGACCC
TAAGCAAGCGATCGAGGCACTCCGTGACGCTGAACCGACACCGATGGATATCGGCAAATATGAGAACGGTTATTTCATGA
ACGTCATCGCCATCGGGCTCATTGCCGAAGCCGTCGACGAAGTAAGTGTGGAAGAGAAGACGAAATGGGGACCGTTCGCT
TACTTGATTGAGGGTGTAAAAGCGTTCCGTGAGCATAGTCCATATGAGTTGGCACTTGACAGTACAGACGGTCAGTTTAA
TGGCGAAGCCTATCTCGTCGTCATCGCCTTGACGAACTCAGTCGGAGGATTTGAAAACTTCGAACCAGACGCGCGGTTGA
ATGATGGGCTTCTTCATGTCTATATCTTTGAAGAACTCGGGTTAAAGGACGCACTTCAATTAACACCCGCCCTCTTCACA
GGAAAGTTGAAGGAAACAGATAGCGTCACCTCGTTTTGTACGAAACGGGTTAAGGTGACCTCACCCGAGGCGCTTCCTGT
AAATGCTGACGGGGACACAGGGGGCACGCTCCCACTTACTTTTGAAGTATTGCCGAGCCATTTAAACGTATTAAAGCCGG
TCTAA

Upstream 100 bases:

>100_bases
AGACCTAAATAGCTAATCGACATGGGGATTTTTTAGGAGTATCTGTTTTCTTTGACGGGAATAAAGGACAGTATTCGAGT
TGAAAAGGAGACATGTTGGA

Downstream 100 bases:

>100_bases
GAGCAGGAGTTTTGACGATTGAATCGAATGTGTTCAAGCGAGAAAGGAGAATGACCTATCAACATCTCGCTTGTTCCATT
AAAAACTGCATCCGTCGACA

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDTVMLIVNPSSGKELGEQHATHAEEVLRERYGHVDVRFTEKEQDATNFAREAAQKHYQAVIAMGGDGTLNEAVTGLAEA
TYRPDFGIIPLGTVNDLARALGVPSDPKQAIEALRDAEPTPMDIGKYENGYFMNVIAIGLIAEAVDEVSVEEKTKWGPFA
YLIEGVKAFREHSPYELALDSTDGQFNGEAYLVVIALTNSVGGFENFEPDARLNDGLLHVYIFEELGLKDALQLTPALFT
GKLKETDSVTSFCTKRVKVTSPEALPVNADGDTGGTLPLTFEVLPSHLNVLKPV

Sequences:

>Translated_294_residues
MDTVMLIVNPSSGKELGEQHATHAEEVLRERYGHVDVRFTEKEQDATNFAREAAQKHYQAVIAMGGDGTLNEAVTGLAEA
TYRPDFGIIPLGTVNDLARALGVPSDPKQAIEALRDAEPTPMDIGKYENGYFMNVIAIGLIAEAVDEVSVEEKTKWGPFA
YLIEGVKAFREHSPYELALDSTDGQFNGEAYLVVIALTNSVGGFENFEPDARLNDGLLHVYIFEELGLKDALQLTPALFT
GKLKETDSVTSFCTKRVKVTSPEALPVNADGDTGGTLPLTFEVLPSHLNVLKPV
>Mature_294_residues
MDTVMLIVNPSSGKELGEQHATHAEEVLRERYGHVDVRFTEKEQDATNFAREAAQKHYQAVIAMGGDGTLNEAVTGLAEA
TYRPDFGIIPLGTVNDLARALGVPSDPKQAIEALRDAEPTPMDIGKYENGYFMNVIAIGLIAEAVDEVSVEEKTKWGPFA
YLIEGVKAFREHSPYELALDSTDGQFNGEAYLVVIALTNSVGGFENFEPDARLNDGLLHVYIFEELGLKDALQLTPALFT
GKLKETDSVTSFCTKRVKVTSPEALPVNADGDTGGTLPLTFEVLPSHLNVLKPV

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=294, Percent_Identity=27.2108843537415, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 31968; Mature: 31968

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVMLIVNPSSGKELGEQHATHAEEVLRERYGHVDVRFTEKEQDATNFAREAAQKHYQA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHE
VIAMGGDGTLNEAVTGLAEATYRPDFGIIPLGTVNDLARALGVPSDPKQAIEALRDAEPT
EEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCC
PMDIGKYENGYFMNVIAIGLIAEAVDEVSVEEKTKWGPFAYLIEGVKAFREHSPYELALD
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEE
STDGQFNGEAYLVVIALTNSVGGFENFEPDARLNDGLLHVYIFEELGLKDALQLTPALFT
CCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHC
GKLKETDSVTSFCTKRVKVTSPEALPVNADGDTGGTLPLTFEVLPSHLNVLKPV
CCCCCCHHHHHHHHHCEEECCCCEEEECCCCCCCCCCEEEHEEHHHHHHHHCCC
>Mature Secondary Structure
MDTVMLIVNPSSGKELGEQHATHAEEVLRERYGHVDVRFTEKEQDATNFAREAAQKHYQA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHE
VIAMGGDGTLNEAVTGLAEATYRPDFGIIPLGTVNDLARALGVPSDPKQAIEALRDAEPT
EEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCC
PMDIGKYENGYFMNVIAIGLIAEAVDEVSVEEKTKWGPFAYLIEGVKAFREHSPYELALD
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEE
STDGQFNGEAYLVVIALTNSVGGFENFEPDARLNDGLLHVYIFEELGLKDALQLTPALFT
CCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHC
GKLKETDSVTSFCTKRVKVTSPEALPVNADGDTGGTLPLTFEVLPSHLNVLKPV
CCCCCCHHHHHHHHHCEEECCCCEEEECCCCCCCCCCEEEHEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA