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The map label for this gene is exp5 [H]

Identifier: 229917707

GI number: 229917707

Start: 1941686

End: 1943314

Strand: Reverse

Name: exp5 [H]

Synonym: EAT1b_1984

Alternate gene names: 229917707

Gene position: 1943314-1941686 (Counterclockwise)

Preceding gene: 229917711

Following gene: 229917706

Centisome position: 64.78

GC content: 50.64

Gene sequence:

>1629_bases
ATGAAACAATTGATCTCATTCGATTTCTGGCAAAAGCTCGGTAAGGCCTTGATGGTCGTTATCGCCGTAATGCCAGCTGC
CGGTTTGATGATTTCCATCGGTAAATTGATTGGAATGTCAGCCGGAGATGTCGGTATTTTATTAACAACAGCTCGCATCA
TTGAAGATTTAGGTTGGGGCATCATCGTCAACTTACATATCCTCTTCGCAGTCGCGATTGGTGGTTCGTGGGCAAAAGAT
CGTGCAGGTGGTGCGTTCGCAGCACTTCTCGCCTTCATCTTGATCAACCGTGTCACAGGTGCCATCTTCGGTGTTAACGG
GGCCATGCTTCAAGATCCGGAAGCAACCATCGAGTCACTCTTCGGTGCGAACCTTGTCGTCGCTGACTACTTCACATCTG
TACTCGGCTCACCTGCCCTCAACATGGGTGTGTTCGTCGGGATCATCGCCGGTTTCCTCGGTGGTGCACTATACAACCGT
TTCTACAACTTCAACAAGTTACCGAACGCCCTCTCGTTCTTCAACGGGAAGCGCTTCGTACCATTCGTCGTCATCCTTGG
TTCAGTCGTTGCTGCCATCGTTCTATCGATTGTATGGCCAACTATCCAAGGGGCGCTCAACAGCTTTGGTGAATGGATTG
CCACATCACGTAACACGGCGCCTGTCCTCGCCCCGTTCATTTACGGTGCGCTCGAACGTCTCCTCTTGCCGTTCGGTTTA
CACCACATGCTCACGGTACCAATGAACTACACGGAGCTCGGTGGAACGTACACGATTTTAACTGGCGCGACAGCTGGATC
GGTCGTTGCCGGTCAAGACCCGCTCTGGTTGGCATGGGTAACGGACCTCGTCAACTTGCGTGCTGCTGGTGATACGGCTG
CGTACAATGCTCTTCTCGCTGATGTCGTTCCGGCCCGCTTCAAAGTCGGTCAAGTCATCTTGTCATTCGCTGCATTGATT
GGTGCCGCATATGCGATGTACCGCAACGTCGATGCGGATAAGAAAGCACAATACAAGCCGATGTTCTTGTCAGCTGGACT
CGCTGTCTTCTTGACAGGTGTCACAGAACCGATCGAATTCATGTTCATGTTCGTCGCACCAGTCCTTTATGTGGTGTACG
CGCTCATGGCCGGCGCTGCCTTCGCATTGGCAGATATCATCGACCTACGCGTCCACGCTTTCGGTGCAATCGAACTCTTG
ACGCGCATTCCGATGATCGTCAAAGCAGGACTTCTTCAAGACTTGATCAACTTCGTTCTCGTCTCTGTCGTGTTCTTCTT
CCTCAACTTTGGTGTCTTCAACTTCTTCATTAAGAAGATGAACTTGCCGACACCTGGACGTAACGGGAACTACATGGAAG
AAACGACAGAAAAATCATCCGTTACAGGTAACGAACAAGTCATGGGGATTATCGCACTTCTCGGTGGGGAAGAAAACATT
GAGGATGTCGATGCATGTATGACGCGTCTTCGTGTGACCGTGAAAGACGTTACACTCGTCTCTGACGAACAAGAATGGAA
GAAAAATGGCGCCATGGGTCTTATCGTGAAAGACCGTGGAGTCCAAGCCATCTATGGCCCGAAAGCCGACGTATTGAAAT
CAGACATTCAAGACGTCATCGGTGCATAA

Upstream 100 bases:

>100_bases
ATGAATTTATGCAATCGATTACAATCATTTATGCAAGCGATTGCACTATTGGCAAACACAAAAATAGATAAAACTCCCTC
ACAGGAAGGAAGCGTATAGC

Downstream 100 bases:

>100_bases
TCTACTGGCCAACTCGCTCGAGAGTTGGCCTTTTTCTAAAGGAGGACTATCGTGAATATTTTAACGCTCAATTGTCATGC
CTGGTTAGAAGACCGCCAAC

Product: PTS system, IIBC subunit

Products: NA

Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKQLISFDFWQKLGKALMVVIAVMPAAGLMISIGKLIGMSAGDVGILLTTARIIEDLGWGIIVNLHILFAVAIGGSWAKD
RAGGAFAALLAFILINRVTGAIFGVNGAMLQDPEATIESLFGANLVVADYFTSVLGSPALNMGVFVGIIAGFLGGALYNR
FYNFNKLPNALSFFNGKRFVPFVVILGSVVAAIVLSIVWPTIQGALNSFGEWIATSRNTAPVLAPFIYGALERLLLPFGL
HHMLTVPMNYTELGGTYTILTGATAGSVVAGQDPLWLAWVTDLVNLRAAGDTAAYNALLADVVPARFKVGQVILSFAALI
GAAYAMYRNVDADKKAQYKPMFLSAGLAVFLTGVTEPIEFMFMFVAPVLYVVYALMAGAAFALADIIDLRVHAFGAIELL
TRIPMIVKAGLLQDLINFVLVSVVFFFLNFGVFNFFIKKMNLPTPGRNGNYMEETTEKSSVTGNEQVMGIIALLGGEENI
EDVDACMTRLRVTVKDVTLVSDEQEWKKNGAMGLIVKDRGVQAIYGPKADVLKSDIQDVIGA

Sequences:

>Translated_542_residues
MKQLISFDFWQKLGKALMVVIAVMPAAGLMISIGKLIGMSAGDVGILLTTARIIEDLGWGIIVNLHILFAVAIGGSWAKD
RAGGAFAALLAFILINRVTGAIFGVNGAMLQDPEATIESLFGANLVVADYFTSVLGSPALNMGVFVGIIAGFLGGALYNR
FYNFNKLPNALSFFNGKRFVPFVVILGSVVAAIVLSIVWPTIQGALNSFGEWIATSRNTAPVLAPFIYGALERLLLPFGL
HHMLTVPMNYTELGGTYTILTGATAGSVVAGQDPLWLAWVTDLVNLRAAGDTAAYNALLADVVPARFKVGQVILSFAALI
GAAYAMYRNVDADKKAQYKPMFLSAGLAVFLTGVTEPIEFMFMFVAPVLYVVYALMAGAAFALADIIDLRVHAFGAIELL
TRIPMIVKAGLLQDLINFVLVSVVFFFLNFGVFNFFIKKMNLPTPGRNGNYMEETTEKSSVTGNEQVMGIIALLGGEENI
EDVDACMTRLRVTVKDVTLVSDEQEWKKNGAMGLIVKDRGVQAIYGPKADVLKSDIQDVIGA
>Mature_542_residues
MKQLISFDFWQKLGKALMVVIAVMPAAGLMISIGKLIGMSAGDVGILLTTARIIEDLGWGIIVNLHILFAVAIGGSWAKD
RAGGAFAALLAFILINRVTGAIFGVNGAMLQDPEATIESLFGANLVVADYFTSVLGSPALNMGVFVGIIAGFLGGALYNR
FYNFNKLPNALSFFNGKRFVPFVVILGSVVAAIVLSIVWPTIQGALNSFGEWIATSRNTAPVLAPFIYGALERLLLPFGL
HHMLTVPMNYTELGGTYTILTGATAGSVVAGQDPLWLAWVTDLVNLRAAGDTAAYNALLADVVPARFKVGQVILSFAALI
GAAYAMYRNVDADKKAQYKPMFLSAGLAVFLTGVTEPIEFMFMFVAPVLYVVYALMAGAAFALADIIDLRVHAFGAIELL
TRIPMIVKAGLLQDLINFVLVSVVFFFLNFGVFNFFIKKMNLPTPGRNGNYMEETTEKSSVTGNEQVMGIIALLGGEENI
EDVDACMTRLRVTVKDVTLVSDEQEWKKNGAMGLIVKDRGVQAIYGPKADVLKSDIQDVIGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787343, Length=539, Percent_Identity=33.7662337662338, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1787908, Length=544, Percent_Identity=28.8602941176471, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786894, Length=544, Percent_Identity=33.6397058823529, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011300 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 58183; Mature: 58183

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLISFDFWQKLGKALMVVIAVMPAAGLMISIGKLIGMSAGDVGILLTTARIIEDLGWG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
IIVNLHILFAVAIGGSWAKDRAGGAFAALLAFILINRVTGAIFGVNGAMLQDPEATIESL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHH
FGANLVVADYFTSVLGSPALNMGVFVGIIAGFLGGALYNRFYNFNKLPNALSFFNGKRFV
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHH
PFVVILGSVVAAIVLSIVWPTIQGALNSFGEWIATSRNTAPVLAPFIYGALERLLLPFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
HHMLTVPMNYTELGGTYTILTGATAGSVVAGQDPLWLAWVTDLVNLRAAGDTAAYNALLA
HHHEECCCCHHHCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
DVVPARFKVGQVILSFAALIGAAYAMYRNVDADKKAQYKPMFLSAGLAVFLTGVTEPIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
MFMFVAPVLYVVYALMAGAAFALADIIDLRVHAFGAIELLTRIPMIVKAGLLQDLINFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVVFFFLNFGVFNFFIKKMNLPTPGRNGNYMEETTEKSSVTGNEQVMGIIALLGGEENI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHCCCCCHHHHHHHHHHCCCCCH
EDVDACMTRLRVTVKDVTLVSDEQEWKKNGAMGLIVKDRGVQAIYGPKADVLKSDIQDVI
HHHHHHHHHHHHHHHHEEEECCHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHH
GA
CC
>Mature Secondary Structure
MKQLISFDFWQKLGKALMVVIAVMPAAGLMISIGKLIGMSAGDVGILLTTARIIEDLGWG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
IIVNLHILFAVAIGGSWAKDRAGGAFAALLAFILINRVTGAIFGVNGAMLQDPEATIESL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHH
FGANLVVADYFTSVLGSPALNMGVFVGIIAGFLGGALYNRFYNFNKLPNALSFFNGKRFV
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHH
PFVVILGSVVAAIVLSIVWPTIQGALNSFGEWIATSRNTAPVLAPFIYGALERLLLPFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
HHMLTVPMNYTELGGTYTILTGATAGSVVAGQDPLWLAWVTDLVNLRAAGDTAAYNALLA
HHHEECCCCHHHCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
DVVPARFKVGQVILSFAALIGAAYAMYRNVDADKKAQYKPMFLSAGLAVFLTGVTEPIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
MFMFVAPVLYVVYALMAGAAFALADIIDLRVHAFGAIELLTRIPMIVKAGLLQDLINFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVVFFFLNFGVFNFFIKKMNLPTPGRNGNYMEETTEKSSVTGNEQVMGIIALLGGEENI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHCCCCCHHHHHHHHHHCCCCCH
EDVDACMTRLRVTVKDVTLVSDEQEWKKNGAMGLIVKDRGVQAIYGPKADVLKSDIQDVI
HHHHHHHHHHHHHHHHEEEECCHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHH
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 7934910 [H]