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The map label for this gene is alkK [H]

Identifier: 227827150

GI number: 227827150

Start: 776671

End: 778347

Strand: Direct

Name: alkK [H]

Synonym: M1425_0825

Alternate gene names: 227827150

Gene position: 776671-778347 (Clockwise)

Preceding gene: 227827149

Following gene: 227827151

Centisome position: 29.77

GC content: 36.67

Gene sequence:

>1677_bases
ATGATGGCTAAAGTCTTCATAAAGGGTTTACCATCAACAATGATGGATGATTTTCAATTAAATGTAATTAATTTGCTTCA
GCATGCTGCAGAAAACTATGGAGAGAGGGAAATAAAGTCTAGAAAATTAGATGGTAAATTAGAAGTATTTAATTATAAGA
CTATTTACGAGAGGGTAAAGAGACTAGCAAATGCATTAGAATCATTAGGAGTTAAACCTACTGATAGAGTTGGAGTATTA
GGATTTAACACTCACAGATATTTTGAAGCCTATTTCGGCATAAGCGGTATTGGTGCAGTAATGGTTGAGATGAACTTCAG
ACTCTCACCGGATGAATTATCCTACATTGCTAAACACTCTAAAGTTAAAGTAATATTTGCGGATGAGCAATTAATACCGA
TAGTGGAGAAAGTTAACGATAAGGTGAACTCCGATAAGGTCGTGATTATGACCGATAAAGAAAGAACACCCTCTACTAAA
ATTTCTAAAGTTTATCACTATGAGGACTTACTTAAAAGTAGTTCTCCAAATTACGACTTCCCTATAATCGATGAGAAATC
AGCTTGTTTTGCTTGCTACACTTCCGGTACTACTGGAATGCCTAAAGGAGTTTATTATTCACATAGGGCCATAGTATTAC
ATTCTATGGCAGTAATTCAAGCAGTTCCCGTAAACGCTAATGACGCGCTTTTACAGTTAGTTCCTATGTTTCACGCAAAC
GGCTGGGGGTGGCCTTTTACCGCTACCATGGTTGGAGCTAAGCAAGTATTTCCTGGGCAATACAGTTTAGATAACTTAAA
GCCTATCATTGATCTGCTTATAAACGAAAATATCACAATATCTAATGGAGTTCCAACTCTGTGGATAGCAATTCATAACT
ATTTATCGTCACTAAAAGATAAGCCTAAATTTAACAATTTGAAAGTACTTATAGGCGGAAGCGAGCCTCCTTTAGCCTTA
ATAAAGGGGCTTAGGGAGTTCGGAATAGAGGTAATACACGGTTATGGGTCAACTGAAACTACGCCTCTATCCCATATAAA
CGTAATAAAGTACTATTTAGGATTAAGTCAGGAGGAATATGAGGAGTTAAAATCCAAGCAAGGAATTCCAGTATTTCCGA
TACAAAGAAAAACAGTAACTCCAGATGGAAGAGATATACCTTGGGATAATAAAACCATTGGAGAACTTTTAGAGAGAGGG
CCTTGGGTGGTTAAAGAATATTATAATGACCCAAGAACGTTAGAATCTTTCATAGGAGAGGGTTATGAAATTTGGTGGAA
GAGCGGCAATGCTGTTACTGTAGATAAGTACGGTTATATAAAGATAGCAGATAGGCTTAAAGATCTTATAAAGAGCGGAG
GTGAGTGGATATCAAGCGTAGATATGGAGAACTTCTTGATGGCTCATCCTAAGGTTCTTGAGGCTACAGTAGTTGGCGTC
CCTCATCCTAAATGGCAAGAGAGACCTTTAGCCTTTGTCGTTGTAAAACCAGAGTATAAGGGTAACGTAACTAAGGAAGA
ACTTTTAGAACATTTATTAAAGAGATTAGCAAAGTGGCAATTACCCGAAATAATTTTTACGGATTCCATCCCTAAAACCA
GTACTGGTAAGTTCGATAAAAAGGTATTGAGAGAACAATATAAGGATTATTTCATAAAAAGAGAAATGATAGAGTAA

Upstream 100 bases:

>100_bases
TATCCCTTTTATATGACGTTAAAAGTCAATTAACTCCTTAATAGCAACGTGTTCATATTAAGTGGAAGAAATACTTAAAA
TAGTTAAATTATATAAATAA

Downstream 100 bases:

>100_bases
GTTCTTGCTAAAAGTTCACGTTCTTTTTCTTTTAATTTCTTCAATTACTGGCTTAGATAGTTCCATAAGCGTATAGAAGG
CATCAAGATTAGAGATCACG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MMAKVFIKGLPSTMMDDFQLNVINLLQHAAENYGEREIKSRKLDGKLEVFNYKTIYERVKRLANALESLGVKPTDRVGVL
GFNTHRYFEAYFGISGIGAVMVEMNFRLSPDELSYIAKHSKVKVIFADEQLIPIVEKVNDKVNSDKVVIMTDKERTPSTK
ISKVYHYEDLLKSSSPNYDFPIIDEKSACFACYTSGTTGMPKGVYYSHRAIVLHSMAVIQAVPVNANDALLQLVPMFHAN
GWGWPFTATMVGAKQVFPGQYSLDNLKPIIDLLINENITISNGVPTLWIAIHNYLSSLKDKPKFNNLKVLIGGSEPPLAL
IKGLREFGIEVIHGYGSTETTPLSHINVIKYYLGLSQEEYEELKSKQGIPVFPIQRKTVTPDGRDIPWDNKTIGELLERG
PWVVKEYYNDPRTLESFIGEGYEIWWKSGNAVTVDKYGYIKIADRLKDLIKSGGEWISSVDMENFLMAHPKVLEATVVGV
PHPKWQERPLAFVVVKPEYKGNVTKEELLEHLLKRLAKWQLPEIIFTDSIPKTSTGKFDKKVLREQYKDYFIKREMIE

Sequences:

>Translated_558_residues
MMAKVFIKGLPSTMMDDFQLNVINLLQHAAENYGEREIKSRKLDGKLEVFNYKTIYERVKRLANALESLGVKPTDRVGVL
GFNTHRYFEAYFGISGIGAVMVEMNFRLSPDELSYIAKHSKVKVIFADEQLIPIVEKVNDKVNSDKVVIMTDKERTPSTK
ISKVYHYEDLLKSSSPNYDFPIIDEKSACFACYTSGTTGMPKGVYYSHRAIVLHSMAVIQAVPVNANDALLQLVPMFHAN
GWGWPFTATMVGAKQVFPGQYSLDNLKPIIDLLINENITISNGVPTLWIAIHNYLSSLKDKPKFNNLKVLIGGSEPPLAL
IKGLREFGIEVIHGYGSTETTPLSHINVIKYYLGLSQEEYEELKSKQGIPVFPIQRKTVTPDGRDIPWDNKTIGELLERG
PWVVKEYYNDPRTLESFIGEGYEIWWKSGNAVTVDKYGYIKIADRLKDLIKSGGEWISSVDMENFLMAHPKVLEATVVGV
PHPKWQERPLAFVVVKPEYKGNVTKEELLEHLLKRLAKWQLPEIIFTDSIPKTSTGKFDKKVLREQYKDYFIKREMIE
>Mature_558_residues
MMAKVFIKGLPSTMMDDFQLNVINLLQHAAENYGEREIKSRKLDGKLEVFNYKTIYERVKRLANALESLGVKPTDRVGVL
GFNTHRYFEAYFGISGIGAVMVEMNFRLSPDELSYIAKHSKVKVIFADEQLIPIVEKVNDKVNSDKVVIMTDKERTPSTK
ISKVYHYEDLLKSSSPNYDFPIIDEKSACFACYTSGTTGMPKGVYYSHRAIVLHSMAVIQAVPVNANDALLQLVPMFHAN
GWGWPFTATMVGAKQVFPGQYSLDNLKPIIDLLINENITISNGVPTLWIAIHNYLSSLKDKPKFNNLKVLIGGSEPPLAL
IKGLREFGIEVIHGYGSTETTPLSHINVIKYYLGLSQEEYEELKSKQGIPVFPIQRKTVTPDGRDIPWDNKTIGELLERG
PWVVKEYYNDPRTLESFIGEGYEIWWKSGNAVTVDKYGYIKIADRLKDLIKSGGEWISSVDMENFLMAHPKVLEATVVGV
PHPKWQERPLAFVVVKPEYKGNVTKEELLEHLLKRLAKWQLPEIIFTDSIPKTSTGKFDKKVLREQYKDYFIKREMIE

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=540, Percent_Identity=23.8888888888889, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI187761345, Length=535, Percent_Identity=24.6728971962617, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI187761343, Length=535, Percent_Identity=24.6728971962617, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI42544132, Length=571, Percent_Identity=24.5183887915937, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=23.7451737451737, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=23.1499051233397, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157311624, Length=523, Percent_Identity=23.3269598470363, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=523, Percent_Identity=23.3269598470363, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI83745141, Length=508, Percent_Identity=24.6062992125984, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=23.062015503876, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI115511026, Length=523, Percent_Identity=23.7093690248566, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI27477105, Length=511, Percent_Identity=24.853228962818, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=147, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=27.3972602739726, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=26.6791044776119, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=24.3713733075435, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=22.7005870841487, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1790505, Length=564, Percent_Identity=23.4042553191489, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788595, Length=524, Percent_Identity=23.4732824427481, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789201, Length=446, Percent_Identity=21.9730941704036, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786801, Length=439, Percent_Identity=23.0068337129841, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=519, Percent_Identity=27.3603082851638, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=546, Percent_Identity=25.8241758241758, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=24.282982791587, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=521, Percent_Identity=26.1036468330134, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=24.2201834862385, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=24.2201834862385, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=24.2201834862385, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=533, Percent_Identity=22.7016885553471, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=516, Percent_Identity=24.2248062015504, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=25.1923076923077, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=511, Percent_Identity=25.0489236790607, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=507, Percent_Identity=22.0907297830375, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17551278, Length=528, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=24.8633879781421, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115534440, Length=518, Percent_Identity=22.5868725868726, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=538, Percent_Identity=21.003717472119, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=538, Percent_Identity=21.003717472119, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=28.099173553719, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=538, Percent_Identity=22.8624535315985, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581924, Length=549, Percent_Identity=28.0510018214936, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21355181, Length=530, Percent_Identity=27.5471698113208, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI18859661, Length=493, Percent_Identity=27.1805273833671, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472339, Length=581, Percent_Identity=23.0636833046472, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667955, Length=551, Percent_Identity=23.049001814882, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922652, Length=506, Percent_Identity=22.5296442687747, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648260, Length=515, Percent_Identity=24.0776699029126, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24667959, Length=511, Percent_Identity=22.7005870841487, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17933690, Length=499, Percent_Identity=22.8456913827655, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356947, Length=509, Percent_Identity=24.5579567779961, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653035, Length=498, Percent_Identity=22.8915662650602, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 63467; Mature: 63467

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKVFIKGLPSTMMDDFQLNVINLLQHAAENYGEREIKSRKLDGKLEVFNYKTIYERVK
CCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEHHHHHHHHH
RLANALESLGVKPTDRVGVLGFNTHRYFEAYFGISGIGAVMVEMNFRLSPDELSYIAKHS
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEEEEEECEECHHHHHHHHHCC
KVKVIFADEQLIPIVEKVNDKVNSDKVVIMTDKERTPSTKISKVYHYEDLLKSSSPNYDF
EEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
PIIDEKSACFACYTSGTTGMPKGVYYSHRAIVLHSMAVIQAVPVNANDALLQLVPMFHAN
CEECCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCC
GWGWPFTATMVGAKQVFPGQYSLDNLKPIIDLLINENITISNGVPTLWIAIHNYLSSLKD
CCCCCHHHHHHCHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCC
KPKFNNLKVLIGGSEPPLALIKGLREFGIEVIHGYGSTETTPLSHINVIKYYLGLSQEEY
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHH
EELKSKQGIPVFPIQRKTVTPDGRDIPWDNKTIGELLERGPWVVKEYYNDPRTLESFIGE
HHHHHCCCCCEECCCCCEECCCCCCCCCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHCC
GYEIWWKSGNAVTVDKYGYIKIADRLKDLIKSGGEWISSVDMENFLMAHPKVLEATVVGV
CEEEEEECCCEEEEECCCEEEHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCEEEEEEECC
PHPKWQERPLAFVVVKPEYKGNVTKEELLEHLLKRLAKWQLPEIIFTDSIPKTSTGKFDK
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
KVLREQYKDYFIKREMIE
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMAKVFIKGLPSTMMDDFQLNVINLLQHAAENYGEREIKSRKLDGKLEVFNYKTIYERVK
CCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEHHHHHHHHH
RLANALESLGVKPTDRVGVLGFNTHRYFEAYFGISGIGAVMVEMNFRLSPDELSYIAKHS
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEEEEEECEECHHHHHHHHHCC
KVKVIFADEQLIPIVEKVNDKVNSDKVVIMTDKERTPSTKISKVYHYEDLLKSSSPNYDF
EEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
PIIDEKSACFACYTSGTTGMPKGVYYSHRAIVLHSMAVIQAVPVNANDALLQLVPMFHAN
CEECCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCC
GWGWPFTATMVGAKQVFPGQYSLDNLKPIIDLLINENITISNGVPTLWIAIHNYLSSLKD
CCCCCHHHHHHCHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCC
KPKFNNLKVLIGGSEPPLALIKGLREFGIEVIHGYGSTETTPLSHINVIKYYLGLSQEEY
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHH
EELKSKQGIPVFPIQRKTVTPDGRDIPWDNKTIGELLERGPWVVKEYYNDPRTLESFIGE
HHHHHCCCCCEECCCCCEECCCCCCCCCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHCC
GYEIWWKSGNAVTVDKYGYIKIADRLKDLIKSGGEWISSVDMENFLMAHPKVLEATVVGV
CEEEEEECCCEEEEECCCEEEHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCEEEEEEECC
PHPKWQERPLAFVVVKPEYKGNVTKEELLEHLLKRLAKWQLPEIIFTDSIPKTSTGKFDK
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
KVLREQYKDYFIKREMIE
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]