Click here to switch to the map view.

The map label for this gene is pucI [H]

Identifier: 227827044

GI number: 227827044

Start: 655834

End: 657312

Strand: Direct

Name: pucI [H]

Synonym: M1425_0703

Alternate gene names: 227827044

Gene position: 655834-657312 (Clockwise)

Preceding gene: 227827039

Following gene: 227827048

Centisome position: 25.14

GC content: 37.39

Gene sequence:

>1479_bases
ATGTCAGAACAAGAAATAAACCACGTTAATTTAGACTTAACGGAATATAATCAAGGAACGACTGTAGTACCAGACAGTTA
CTATAATCCAAACATAGCGCCGCTTCCTAAAAACGCAAAAACATGGACATGGATAAATTATGCTACCATATGGGCTGGAA
TGATACATAACGTCCCCGCATTTATGCTTGCGGGGCTATTAACATTTGAGTTCGGCCCACTAATAGCATTAATGATCATC
GCAATAGCCTACTTTACCTTGCTAATAGCGCTATACTTAAATGGGCATATAGGTACAAAATGGGGAATTCCATTCCCCTC
ATCAATTAGACCAATGTTCGGAATAAGGGGTGCTAGAATACCAGTAATAATGAGGGCAATTTCAGCATTGTTTTGGTTCT
CCGTTGAGACCTATGCTGGCGGTCTAATATTAGATGCGCTCATCTCAATCTTTTATCCCTCATGGTCAACAATCTCAGCA
GACCTCTTAGGAATGCCACTCCATCTGGCAATTTCGTTCTTCCTCTTTTGGTTCCTTAACGTATTAGTCTTATTTAAGGG
AATGGATGATATAAAGAAATTTGAACTAATTGCTGGTCCCTTGGTAATAATAATCTTAGGAGGTTTGATGATTCACGTAA
TTACTCTTGCAAATGGTCTATCATCATTGTTTCAAATAAGGGGGGATAACGTTTCATTACCTAACATAGCCTTAGCAATA
TCCACAATGGCAGGTTTTTGGGCAACCCTAGTCCTAAACATTCCGGACTTTACGAGATTTTCTAGAAGCCAAAAGGACCA
ACTAATAGGACAAACTGTTGGTCTACCTATACTTACGTTGCTTTTCAGCTTCATAGCAGTTGGGTTAGCATCGGCAGTAA
TTTATATTTACAATATTCCAAGTAATGACGCAATTAATTATGTAAACCCAGTAAATATAATGTATCTCTTTACTGACAAT
CCTTACATAACGTTAATCTTAGGAATCAGTCTAGTTATTGCAACAATCTCAGTTAACGTTGCAGCAAATATTGTATCACC
CGTTTACGACTTGATAAGTTTATTCCCAAAGAAGCTTAACACGTGGTCTAAATCAGCTATTGTATCTGCAATTCTGGGTT
TACTTTACGCCCCATGGTTATGGTACAATAACGCTTCAAGTATAGAAAATGTGATAAATTTGATTGGTGCCGGTCTAGGT
TCTGTTGCCGGAGTCATGATAGCCCACTACTGGATATTAGGAAAAACTGAAATTAAACTTGCAGATCTATTTAAGCCAAA
TGGAAGATATTGGTATGTGTCAGGCTATAACGTTAATGCGTTAGTTGCAATGATCATAGGGTTCTCTGTACCAGTAATAG
GATTTCTAATTCCTAAACTATCCTTGCTATATGACTATGGTTGGTATCTTGGATTATTTTTGAGTATAGCAATATATTTG
GGATTAGAGAGAAGAAGAGAAGTGAAAATGGAACCTTAA

Upstream 100 bases:

>100_bases
ATTGGAGATTTTTGGTATTTCCTAATTCACGATTAGATTAAAAATTACTCTCTCTTTTAATCAGTTTAATTTATATACCC
CTTTTTAGCAATACTGAAAG

Downstream 100 bases:

>100_bases
TTTTTTATTTCTTTCTAAGTAGATATCTTTGTATTTTACCACTCTCAGTCTTTGGCAATTTATCAACAAAGTCAACCCTT
TCTAGATGTACATGCCTAGA

Product: permease for cytosine/purines uracil thiamine allantoin

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPAFMLAGLLTFEFGPLIALMII
AIAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARIPVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISA
DLLGMPLHLAISFFLFWFLNVLVLFKGMDDIKKFELIAGPLVIIILGGLMIHVITLANGLSSLFQIRGDNVSLPNIALAI
STMAGFWATLVLNIPDFTRFSRSQKDQLIGQTVGLPILTLLFSFIAVGLASAVIYIYNIPSNDAINYVNPVNIMYLFTDN
PYITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLNTWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLG
SVAGVMIAHYWILGKTEIKLADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYL
GLERRREVKMEP

Sequences:

>Translated_492_residues
MSEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPAFMLAGLLTFEFGPLIALMII
AIAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARIPVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISA
DLLGMPLHLAISFFLFWFLNVLVLFKGMDDIKKFELIAGPLVIIILGGLMIHVITLANGLSSLFQIRGDNVSLPNIALAI
STMAGFWATLVLNIPDFTRFSRSQKDQLIGQTVGLPILTLLFSFIAVGLASAVIYIYNIPSNDAINYVNPVNIMYLFTDN
PYITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLNTWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLG
SVAGVMIAHYWILGKTEIKLADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYL
GLERRREVKMEP
>Mature_491_residues
SEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPAFMLAGLLTFEFGPLIALMIIA
IAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARIPVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISAD
LLGMPLHLAISFFLFWFLNVLVLFKGMDDIKKFELIAGPLVIIILGGLMIHVITLANGLSSLFQIRGDNVSLPNIALAIS
TMAGFWATLVLNIPDFTRFSRSQKDQLIGQTVGLPILTLLFSFIAVGLASAVIYIYNIPSNDAINYVNPVNIMYLFTDNP
YITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLNTWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLGS
VAGVMIAHYWILGKTEIKLADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYLG
LERRREVKMEP

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=482, Percent_Identity=30.9128630705394, Blast_Score=202, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322219, Length=483, Percent_Identity=24.0165631469979, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319495, Length=494, Percent_Identity=25.9109311740891, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319429, Length=449, Percent_Identity=25.3897550111359, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324766, Length=441, Percent_Identity=24.2630385487528, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=443, Percent_Identity=22.7990970654628, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324645, Length=439, Percent_Identity=21.1845102505695, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 54487; Mature: 54356

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPA
CCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCHH
FMLAGLLTFEFGPLIALMIIAIAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCHH
PVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISADLLGMPLHLAISFFLFWFLN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
VLVLFKGMDDIKKFELIAGPLVIIILGGLMIHVITLANGLSSLFQIRGDNVSLPNIALAI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
STMAGFWATLVLNIPDFTRFSRSQKDQLIGQTVGLPILTLLFSFIAVGLASAVIYIYNIP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECC
SNDAINYVNPVNIMYLFTDNPYITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLN
CCCCCCCCCCEEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLGSVAGVMIAHYWILGKTEIKL
CHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEE
ADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYL
ECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLERRREVKMEP
HHHHHCCCCCCC
>Mature Secondary Structure 
SEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPA
CCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCHH
FMLAGLLTFEFGPLIALMIIAIAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCHH
PVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISADLLGMPLHLAISFFLFWFLN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
VLVLFKGMDDIKKFELIAGPLVIIILGGLMIHVITLANGLSSLFQIRGDNVSLPNIALAI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
STMAGFWATLVLNIPDFTRFSRSQKDQLIGQTVGLPILTLLFSFIAVGLASAVIYIYNIP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECC
SNDAINYVNPVNIMYLFTDNPYITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLN
CCCCCCCCCCEEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLGSVAGVMIAHYWILGKTEIKL
CHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEE
ADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYL
ECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLERRREVKMEP
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]