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The map label for this gene is proP [H]

Identifier: 227827002

GI number: 227827002

Start: 611180

End: 612469

Strand: Direct

Name: proP [H]

Synonym: M1425_0660

Alternate gene names: 227827002

Gene position: 611180-612469 (Clockwise)

Preceding gene: 227826998

Following gene: 227827004

Centisome position: 23.43

GC content: 34.96

Gene sequence:

>1290_bases
ATGTCAAATAGTGGAGGAAAGGACTGGAGAACAGCACTAAAGGTAGCCATATCAGCCAATTTGGGTTGGGGATTTGAACT
ATTTGATTTAGTAGTTTACCTATATGTTGCAACTACAATATCTCCTCTATTCTTTCCTTCTTCAAGCAAAATAGCTAGCT
TATTGCTTTTTTTATTAACTATAGTTATTGGATATTTTGCTAGACCTTTAGGAGGTATATTCTTTGGACACTATGGAGAT
AAGATAGGAAGGAAGCGAATATGGTTTGTTTCACTGCTTGGAATGGGTATTGCCACTACGCTAATAGGATTTTTGCCCAC
TTATAACCAAGCTGGTATACTTGCCACCTTATTACTGATTTTGTTACGAATATTACAAGGCTTTTTCTTGGCAGGAGAAT
GGGGAGGAGGAATGACGTTAGTTAATGAGTTTTCTCCAAGTAATTTAAGGGGATTAATGAGTGGTATACAGCAAGGAGGA
GCAGCCTTGGGTTTAATATTTGCAGTAATTGCTAGTGAAATAGCTTCATCTTTAGCTCCTGGTGCTGCCTTTACTAAATA
TGGTTGGAGAATTATGTTCTGGTTTGGTATTATTCCATTAATAATAGCTTTAACTGTAAGATGGAAGGTTGGGGAAAGTA
TTGAATGGCTAACTAAGGTAGCAGATAAACCTGAAAAAATTCCTATTGCAACTGTTCTTAAGAAATGGTGGAAGCTGGTT
ATAATAGCTGCTGTAGTGCTGTTTAGTAGTGGATCCATCTATTACGGAATTATAGCTTATATGCCCACGTTTTTTGAACT
TTACACACATTTATCGATATATGAAATAGATAATATTGTATTGGCAACCAATTTAATATGGTTATTCCTATCTCCATTGA
CTGGTTATTTGAGCGATGTATTGAAGATGAGAAAAATACTAATAGCAATACTAGGATTCGTAATTGGTATCCTAATATAT
CCAATAATAAATTTGCTTTATACCGGCTCATATATCTTAAGCATCTTAGTTGGTATGCTAGTAGGATTTTTATTCGCATT
TCAATATTCAATATTTCCTGCTTGGTTATCTGAAAACATAACTACTAATGTGAGATATTCGTACATAGCTTTCTCAATCA
ATTTAGGAGTAGCACTTTCGTCTTTTGCACCGTACATAGTAACAGCGCTAGGGTTAATTTTTCATAATCCAGTTTTAGGT
ATTGCAATTTTTGATATATCTGCTTCAATATTGGGAGGAATAGCTGCATTACTATCGCCCCAAGATAAAGTGGGAATGCA
ACTTAGTTGA

Upstream 100 bases:

>100_bases
TTGTGAACACAATAAATTTGTCTTAACAGTTGAATTCCATTTCAAGTTTTTCGTCGGGAAACATAAGTATTTAAATTTTA
AAATAATATATTTATTTACC

Downstream 100 bases:

>100_bases
AATAAGTCAACTTTTTTCTTCTTCTTTTTTTCTTAGCTCAAATCTCTTTAATTTACCAGTCTCGGTTCTAGGTAATTCCT
TAACGAATTCTATTTCTCTA

Product: major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSNSGGKDWRTALKVAISANLGWGFELFDLVVYLYVATTISPLFFPSSSKIASLLLFLLTIVIGYFARPLGGIFFGHYGD
KIGRKRIWFVSLLGMGIATTLIGFLPTYNQAGILATLLLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGIQQGG
AALGLIFAVIASEIASSLAPGAAFTKYGWRIMFWFGIIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVLKKWWKLV
IIAAVVLFSSGSIYYGIIAYMPTFFELYTHLSIYEIDNIVLATNLIWLFLSPLTGYLSDVLKMRKILIAILGFVIGILIY
PIINLLYTGSYILSILVGMLVGFLFAFQYSIFPAWLSENITTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVLG
IAIFDISASILGGIAALLSPQDKVGMQLS

Sequences:

>Translated_429_residues
MSNSGGKDWRTALKVAISANLGWGFELFDLVVYLYVATTISPLFFPSSSKIASLLLFLLTIVIGYFARPLGGIFFGHYGD
KIGRKRIWFVSLLGMGIATTLIGFLPTYNQAGILATLLLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGIQQGG
AALGLIFAVIASEIASSLAPGAAFTKYGWRIMFWFGIIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVLKKWWKLV
IIAAVVLFSSGSIYYGIIAYMPTFFELYTHLSIYEIDNIVLATNLIWLFLSPLTGYLSDVLKMRKILIAILGFVIGILIY
PIINLLYTGSYILSILVGMLVGFLFAFQYSIFPAWLSENITTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVLG
IAIFDISASILGGIAALLSPQDKVGMQLS
>Mature_428_residues
SNSGGKDWRTALKVAISANLGWGFELFDLVVYLYVATTISPLFFPSSSKIASLLLFLLTIVIGYFARPLGGIFFGHYGDK
IGRKRIWFVSLLGMGIATTLIGFLPTYNQAGILATLLLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGIQQGGA
ALGLIFAVIASEIASSLAPGAAFTKYGWRIMFWFGIIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVLKKWWKLVI
IAAVVLFSSGSIYYGIIAYMPTFFELYTHLSIYEIDNIVLATNLIWLFLSPLTGYLSDVLKMRKILIAILGFVIGILIYP
IINLLYTGSYILSILVGMLVGFLFAFQYSIFPAWLSENITTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVLGI
AIFDISASILGGIAALLSPQDKVGMQLS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=389, Percent_Identity=29.0488431876607, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789941, Length=385, Percent_Identity=33.7662337662338, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=28.2051282051282, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788942, Length=323, Percent_Identity=26.9349845201238, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082231, Length=225, Percent_Identity=29.7777777777778, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082404, Length=312, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46989; Mature: 46858

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSGGKDWRTALKVAISANLGWGFELFDLVVYLYVATTISPLFFPSSSKIASLLLFLLT
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQAGILATLLLI
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGIQQGGAALGLIFAVIASEIASSLAP
HHHHHHHHEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GAAFTKYGWRIMFWFGIIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVLKKWWKLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IIAAVVLFSSGSIYYGIIAYMPTFFELYTHLSIYEIDNIVLATNLIWLFLSPLTGYLSDV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMRKILIAILGFVIGILIYPIINLLYTGSYILSILVGMLVGFLFAFQYSIFPAWLSENI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVLGIAIFDISASILGGIAALLSP
CCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
QDKVGMQLS
CHHCCCCCC
>Mature Secondary Structure 
SNSGGKDWRTALKVAISANLGWGFELFDLVVYLYVATTISPLFFPSSSKIASLLLFLLT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQAGILATLLLI
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGIQQGGAALGLIFAVIASEIASSLAP
HHHHHHHHEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GAAFTKYGWRIMFWFGIIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVLKKWWKLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IIAAVVLFSSGSIYYGIIAYMPTFFELYTHLSIYEIDNIVLATNLIWLFLSPLTGYLSDV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMRKILIAILGFVIGILIYPIINLLYTGSYILSILVGMLVGFLFAFQYSIFPAWLSENI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVLGIAIFDISASILGGIAALLSP
CCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
QDKVGMQLS
CHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]