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The map label for this gene is yiaE [C]

Identifier: 227815737

GI number: 227815737

Start: 2881750

End: 2882769

Strand: Reverse

Name: yiaE [C]

Synonym: BAMEG_3160

Alternate gene names: 227815737

Gene position: 2882769-2881750 (Counterclockwise)

Preceding gene: 227815738

Following gene: 227815734

Centisome position: 55.12

GC content: 36.96

Gene sequence:

>1020_bases
ATGAAAGATAAGAGATATCCTATTTTGTATAGAAGAGGTGTTAGTATAGTGGCAAAAATATTAGTAGCAGGAAAAATTCC
CGAAATCGGATTAGAACTATTAAAAGATCATGATGTGGAAATGTATGATAAAGAGGAGTTAATTTCTTTAGATGAATTAA
CAGAGCGTGTAAAAGACAAAGATGCATTGCTAAGCCTACTTTCTACAAAGGTGACAAAAGAAGTGATTGATGCGGCACCT
AGTTTGAAGATTGTTGCAAACTACGGTGCTGGTTACGATAATATTGATTACACGTATGCTGGAGAAAAAGGGATTGCGGT
AACGAATACACCGAAAGTATCAACGGAAGCGACAGCAGAATTAACATTTGCACTTCTTTTAGCAGCTGCACGTAGAATTC
CTGAAGGTGATACGCTATGTCGTACAACGGGATTTAACGGATGGGCACCGTTATTTTTCTTAGGCCGAGAAGTACATGGT
AAAACAATTGGAATTATTGGCCTTGGAGAAATCGGAAAGGCAGTTGCAAAACGTGCGAAAGCATTTGGAATGAATATTTT
ATATACAGGACCAAATCGAAAACCTGAAGCTGAAAGTGAACTGGAAGCAACATATGTAACATTGGAAGAGTTATTACAAA
CAGCAGACTTTATTACAATTAACTGTGCGTATAATCCGAAATTGCATCATATGATTGATGAAGAGCAGTTTAAAATGATG
AAGAAAACAGCGTATATTGTAAATGCTTCACGTGGACCAATTATGCACGAAGCGGCACTTGCTCATGCGTTAAAAACGAA
TGAAATTGAAGGAGCAGCTCTTGACGTGTTTGAATTTGAGCCGAAAATTACTGAAGAGTTAAAAGGATTAAAGAATGTAG
TACTTGCTCCTCACGTAGGAAATGCAACATTTGAAACGCGTGATGCGATGGCTGAAATGGCAGTAAGAAATATTTTAGCT
GTATTAAAGGGTGAAGAACCTGTAACACCTGTAAATCAAAAAGTATTCGTTACAAAATAA

Upstream 100 bases:

>100_bases
AAAAAGAGGGGAGACTTGCATCTCCTCTTTTTTCATGGAAAAGTGTCGATTTTTAAAACTATAATCAAATTCCTTGGTTT
TTATATGTGGATTTCTTACA

Downstream 100 bases:

>100_bases
AAAGAAACCTTCCAGTTCCGGAAGGTTTCTTTTTATTTTTCTTTATTTGCTCTTTTTGATGGTCTTTGTCTAAGGAATTG
AGATAGCATGTACAAAATAT

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKDKRYPILYRRGVSIVAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAP
SLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHG
KTIGIIGLGEIGKAVAKRAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMM
KKTAYIVNASRGPIMHEAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILA
VLKGEEPVTPVNQKVFVTK

Sequences:

>Translated_339_residues
MKDKRYPILYRRGVSIVAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAP
SLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHG
KTIGIIGLGEIGKAVAKRAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMM
KKTAYIVNASRGPIMHEAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILA
VLKGEEPVTPVNQKVFVTK
>Mature_339_residues
MKDKRYPILYRRGVSIVAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAP
SLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHG
KTIGIIGLGEIGKAVAKRAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMM
KKTAYIVNASRGPIMHEAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILA
VLKGEEPVTPVNQKVFVTK

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=313, Percent_Identity=38.9776357827476, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI23308577, Length=320, Percent_Identity=33.75, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI61743967, Length=320, Percent_Identity=35.3125, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI4557497, Length=320, Percent_Identity=35.3125, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI145580578, Length=334, Percent_Identity=33.2335329341317, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4557499, Length=334, Percent_Identity=33.2335329341317, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI145580575, Length=346, Percent_Identity=32.6589595375723, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=38.4328358208955, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789279, Length=265, Percent_Identity=32.0754716981132, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787645, Length=259, Percent_Identity=31.2741312741313, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788660, Length=257, Percent_Identity=29.1828793774319, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=309, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25147481, Length=318, Percent_Identity=30.5031446540881, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=245, Percent_Identity=37.1428571428571, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6322116, Length=314, Percent_Identity=31.2101910828025, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=31.6455696202532, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324964, Length=322, Percent_Identity=27.0186335403727, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=29.6511627906977, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571528, Length=306, Percent_Identity=40.1960784313725, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28574286, Length=318, Percent_Identity=35.5345911949685, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45552429, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45551003, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI28574284, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24585514, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574282, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19921140, Length=317, Percent_Identity=34.0694006309148, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24646446, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646448, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646452, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646450, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI62472511, Length=319, Percent_Identity=34.1692789968652, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24585516, Length=317, Percent_Identity=29.3375394321767, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 37335; Mature: 37335

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKRYPILYRRGVSIVAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDK
CCCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DALLSLLSTKVTKEVIDAAPSLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCHHHHH
LTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAK
HHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHH
AFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMM
HHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHH
KKTAYIVNASRGPIMHEAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVG
HHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCC
NATFETRDAMAEMAVRNILAVLKGEEPVTPVNQKVFVTK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKDKRYPILYRRGVSIVAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDK
CCCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DALLSLLSTKVTKEVIDAAPSLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCHHHHH
LTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAK
HHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHH
AFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMM
HHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHH
KKTAYIVNASRGPIMHEAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVG
HHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCC
NATFETRDAMAEMAVRNILAVLKGEEPVTPVNQKVFVTK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]