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The map label for this gene is hmp

Identifier: 227815705

GI number: 227815705

Start: 2850486

End: 2851694

Strand: Reverse

Name: hmp

Synonym: BAMEG_3125

Alternate gene names: 227815705

Gene position: 2851694-2850486 (Counterclockwise)

Preceding gene: 227815706

Following gene: 227815703

Centisome position: 54.52

GC content: 34.41

Gene sequence:

>1209_bases
ATGTTAAGCGAAAAAACAATTGAAATCGTAAAGTCAACAGTACCATTATTACAGGAAAAAGGCGTTGAAATTACAACGAG
ATTTTATGAAATTTTATTTTCGGAACATCCGGAGTTATTGAATATTTTCAACCATACAAATCAGAAAAAGGGAAGACAAC
AACAAGCGTTAGCGAATGCTGTTTATGCAGCTGCAACGTACATTGATAATTTAGAAGCTATTATTCCAGTTGTAAAACAA
ATTGGTCATAAGCATAGAAGTTTAGGTATTAAAGCAGAGCATTATCCGATTGTAGGTACATGTTTATTACGTGCAATTAA
AGAGGTCGCAGGTGCACCGGATGAAGTATTAAACGCATGGGGAGAAGCGTATGGTGTCATTGCTGATGCATTCATTAGCA
TTGAAGCAGAGATGTATGAAGAAGCGGCACATAAAGAAGGTGGATGGAAAGATTTCCGTAACTTTGTGGTTGTTAAAAAA
GTGAAGGAAAGCGATGTTATCACGTCATTGTATTTAAAACCTGAAGATGGAGGGAAAGTTTCTTCATTCATTCCAGGACA
ATATGTAACGGTTCAAATAAATATCGAAGGTGAAACATATACACATAATCGTCAATATAGCTTATCAGATGCTCCTGGAA
AAGAATATTATCGTATTAGTGTCAAGAAAGAAAAAGGTGTAGATACACCGGATGGTAAAGTATCTAATTACTTACATGAT
CATGTAAAAGAAGGTGATATGTTACCAGTAAGTGCACCAGCAGGAGATTTCGTATTAAATATGGATTCAACTTTACCAGT
TGTCTTAATTAGTGGTGGAGTAGGTATTACACCAATGATGAGTATGCTAAATACGTTAATTGAACAAGATTCAAAACGTA
ATGTATGTTTTGTTCATGCAGCGATAAATAGTAATACACATGCAATGAAAGAACACGTTGAGGCAGTAGATAATGAATAC
GAACAAGTTAAAGCATATACTTGTTATTCTGCACCAACTGAAAAAGATTTAGAAATGAAGAACTTTGATAAAGAAGGTTT
TGTGGAAAGAGAATGGTTACAAACTATTATTCCGACAACTGAAGCAGAGTTTTATTTCTGTGGTCCAGTACCATTTATGA
AACATATAAATGCTGTACTAACTGATTTAGGTGTGAAACAAGAACATATTCATTATGAATTTTTCGGCCCAGCAGCAAGC
TTACAATAA

Upstream 100 bases:

>100_bases
TTTCACAAAAACACCGTGTTAATAGTGATTAAAATCACAATCTGTATTATACAATTCAGTTAAACTAAAAACAGTTAAGG
AAATAGAGGAGTGGGATACA

Downstream 100 bases:

>100_bases
ATTGAGCTTTAATAAGCAAAAAAAAACGAGGTGTTATTTCACCTCGTTTTTTATTTGTTCTCGTAATAGTCGATTTACTG
TTTCGCGGCGTATACCGATC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSLYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Sequences:

>Translated_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSLYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ
>Mature_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSLYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=45.5919395465995, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1787658, Length=252, Percent_Identity=23.4126984126984, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787098, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=42.1307506053269, Blast_Score=272, Evalue=8e-74,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_BACAN (Q81T23)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843922.1
- RefSeq:   YP_018089.1
- RefSeq:   YP_027626.1
- ProteinModelPortal:   Q81T23
- SMR:   Q81T23
- EnsemblBacteria:   EBBACT00000012677
- EnsemblBacteria:   EBBACT00000016416
- EnsemblBacteria:   EBBACT00000020384
- GeneID:   1084422
- GeneID:   2815527
- GeneID:   2850511
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1467
- KEGG:   bar:GBAA_1467
- KEGG:   bat:BAS1357
- TIGR:   BA_1467
- TIGR:   GBAA_1467
- GeneTree:   EBGT00050000001043
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- ProtClustDB:   PRK13289
- BioCyc:   BANT260799:BAS1357-MONOMER
- BioCyc:   BANT261594:GBAA1467-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10
- PRINTS:   PR00371
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 45008; Mature: 45008

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 95-95 ACT_SITE 135-135 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSLYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEEECCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTEKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
>Mature Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSLYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEEECCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTEKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629