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The map label for this gene is cca

Identifier: 227815617

GI number: 227815617

Start: 2769285

End: 2770478

Strand: Reverse

Name: cca

Synonym: BAMEG_3036

Alternate gene names: 227815617

Gene position: 2770478-2769285 (Counterclockwise)

Preceding gene: 227815618

Following gene: 227815616

Centisome position: 52.97

GC content: 37.69

Gene sequence:

>1194_bases
ATGGAAAGATTTAAAAAAGCTAGTTCTATTATTGAGACTTTAAAGCAGCAAGGACATGAGGCTTACTTTGTCGGTGGAAG
CGTAAGAGACCTTATTATCGATAGGCCGATTGGAGATATTGATATTGCGACATCTGCTTTACCCGAAGAAGTGATGGCTA
TATTTCCAAGACATGTTCCTGTTGGTCTTGAACATGGAACTGTAATTGTAGTAGAAAATGGTGAGCCTTATGAGGTAACC
ACTTTTCGAACAGAGAGTGAATATGAAGATTTTCGAAGACCTAGTAGTGTTCAATTTGTTCGTTCATTAGAAGAGGATTT
AAAACGTCGTGATTTCACAATGAATGCAATTGCCATGACAGAAGAAGGCGAAATGGTCGATTTATTTGCAGGACAGGAAG
CGATTCAAAAGAGAGAAATTGTGACAGTGGGAAATGCTGCGGATCGTTTTCAAGAAGATGCCCTGCGAATGATGCGTGGT
ATTCGGTTCGTAAGTACGTTAGGTTTTTCTTTAGAGACGAAAACGAAACAAGCAATTGAAACATATGGACATTTGCTGGA
ACATATAGCAATTGAGAGAATTACAGTGGAGTTTGAAAAACTGTTAACTGGCACATATTGCGTGAAGGGTCTCAAAGAAT
TAGTGGAAACGAAACTATTTTCTCATCTGCCATATCTACAAATGTCAGAAGAGAGATTATTAAAAGCTACGCAGTATAAC
TGGGATTCTTTTGAAACAGACATTGAGGCATGGGCGTTTTTCTTATATTGTATTGGAGAAGAACATCCATCTGTCTTTTT
ACGTCAATGGAAGTTTTCAAATAAAAAAATAAAAGATATCGTCGCAGTTTTATTAACAATCCGTAAGAGAAAGGAGAAGG
ATTGGGATACAGTCCTTCTTTATAAAACGGGAATTCATATAGCTGAAATGGCAGAAAGAGTATATGAAGCGATGATTGAA
AGCTATGATCATACATCTGTTGAACGGGTACAAACGTTGTTTCAAGCATTACCAATTAAAAGTCGACAAGAGATGGATGT
GACTGGGAATGATTTGTTAAACTGGGCAAGTAAAAAGCCCGGTCCATGGGTTGCTGAAATGATTCAAAAGATTGAGGAAG
CAATCGTACAAGGAAACGTAGTTAATGAGAAAGAGTGTATAAGGGAGTGGCTGCAAGAATGCAATCTACTATAA

Upstream 100 bases:

>100_bases
GGAGAGCGAGCAAGAGAAAGTGTTTACGAGCAGTTTCGCTCTGAAAAAATCGTCTCACAATATGAAACGATTTACTATGA
TGTACTAAGGGATGACAAAA

Downstream 100 bases:

>100_bases
GAAAGCAGTTATTGCAAGTTTTTTCTGAGGCGGATGGAGAGTTTGTATCTGGCCAAACGATTAGTGATAAACTTGGTTGC
TCGAGAACCGCTGTATGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Sequences:

>Translated_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
>Mature_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=32.6086956521739, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=32.6315789473684, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281360112, Length=269, Percent_Identity=29.7397769516729, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357337, Length=269, Percent_Identity=29.7397769516729, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BACAA (C3P5Q6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002866039.1
- ProteinModelPortal:   C3P5Q6
- SMR:   C3P5Q6
- EnsemblBacteria:   EBBACT00000125130
- GeneID:   7850666
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1626
- GeneTree:   EBGT00050000000996
- ProtClustDB:   PRK13299
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45932; Mature: 45932

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 29-29 BINDING 110-110 BINDING 153-153 BINDING 156-156 BINDING 159-159 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
EEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEECCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
EEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEECCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA