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The map label for this gene is narH [H]

Identifier: 227815054

GI number: 227815054

Start: 2252291

End: 2253652

Strand: Reverse

Name: narH [H]

Synonym: BAMEG_2465

Alternate gene names: 227815054

Gene position: 2253652-2252291 (Counterclockwise)

Preceding gene: 227815055

Following gene: 227815053

Centisome position: 43.09

GC content: 38.99

Gene sequence:

>1362_bases
ATGTATTTCAATAACGTAGAAACAAAACCTGGTATTGGTTATCCGAAGCAGTGGGAGGATCAGGAGAAATATAAAGGCGG
CTGGGAATTGAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAAATGAAGCGCCTTATGAATATTTTCCACAATCCAG
ATCAACCAACAATCGATGATTACTTTGAACCGTGGAACTATGATTATGAAACATTAACAACTAGTCCGCAGCGTAAGCAT
CAGCCAGTTGCTCGTCCAAAATCAGCAATTACAGGCGAATTTATCGACAAAATTGAATGGGGTCCAAACTGGGAAGATGA
TTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAGAAAATGGAAGAAGAAATTAAAACCGATTTTGAAA
ATGTTTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAATCCATCTTGCGTATCGTCTTGTCCATCTGGTGCG
ATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTAGATCAAAATGCATGTCGTGCATGGAGATTCTGCGTATCGTCTTG
CCCATATAAAAAAGTGTACGTTAACTGGCAAACGAATAAAGCGGAAAAATGTACAATGTGTTTCCCGCGTATTGAAGCTG
GTATGCCAACAATTTGTTCGGAAACATGTGTAGGGCGTATTCGGTATATTGGAGTAATGCTATATGACGCTGACAAGGTA
AAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTCAGCTTACTGTTTTCCTAGATCCAAATGATCCAGA
AGTAGTAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAATGGATTAAAGCGGCGCAAGAGTCGCCAATCTATAAAATGA
TTATCGATTGGAAAATTGCTCTGCCTCTTCATCCAGAGTATCGTACAATGCCGATGGTTTGGTATATCCCGCCGCTTAGC
CCAATTATGAACATGGTGGAAGGGAAAGGTAGTAACTGGCAAGCAGAAGAGATTTTCCCAGCTATTGATAATATGCGTAT
TCCAATTCAATATTTAGCGAATTTACTCACTGCAGGAGATGAATCACATATTCGACTTACATTGAAAAAAATGGCTGTTA
TGCGAACACATATGAGAGCACTGCAAATTAATAAAGAACCAAATGAGGCTGTATTAAAAGAACTTGGCTTAACGAAACAG
GATGTAGAAGATATGTATCGTCTTCTTGCTATCGCAAAATATAAAGATCGTTTTGTTATCCCGACGTCTCACCGCGAACA
AGTTGCAGACTTATATAACGAACAAGGAAGCTGTGGTTTATCATTTGCAGGTGGTCCAGGTTCTTGTATGACAATTTCTT
AA

Upstream 100 bases:

>100_bases
ACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACGTGCAAAAACACCTGGACAA
ATCGTCCAGGTGCTGAATAT

Downstream 100 bases:

>100_bases
ATAATGGGGGCGGAAAAATATGAAACAAAGTCTACAAATTGCTTTTTCTTGTTCTTCATTCCTTCTCTCTTACCCGGAAC
TTGGGTGGAGAGAAGCTTTA

Product: respiratory nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSFAGGPGSCMTIS

Sequences:

>Translated_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSFAGGPGSCMTIS
>Mature_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSFAGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=445, Percent_Identity=58.4269662921348, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787478, Length=448, Percent_Identity=57.5892857142857, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 51981; Mature: 51981

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTTSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYVNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEEECCCCCCHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
PTSHREQVADLYNEQGSCGLSFAGGPGSCMTIS
CCCHHHHHHHHHCCCCCCEEEECCCCCCEEEEC
>Mature Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTTSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYVNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEEECCCCCCHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEE
PTSHREQVADLYNEQGSCGLSFAGGPGSCMTIS
CCCHHHHHHHHHCCCCCCEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]