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The map label for this gene is nirB [H]

Identifier: 227815035

GI number: 227815035

Start: 2234531

End: 2236936

Strand: Direct

Name: nirB [H]

Synonym: BAMEG_2446

Alternate gene names: 227815035

Gene position: 2234531-2236936 (Clockwise)

Preceding gene: 227815034

Following gene: 227815036

Centisome position: 42.72

GC content: 38.11

Gene sequence:

>2406_bases
ATGAAAAAACGTTTAGTTATGATCGGAAATGGTATGGCTGGCATACGTTGTATGGAAGAAATATTAAAACATGATTGTGA
TTCATATGAGATTACTATTTTTGGAGATGAACCACACCCAAACTATAATCGCATTATGCTCTCTCATGTTTTACAAGGTA
AAACAAATATACAAGATATCATTATGAATGAATACAGTTGGTATGAAGAAAACGAAATAACTTTGTATACAAATGAAAGA
GTTCAAAGTATTGACCGAGAAGAAAAAGTTATTATGACAGAAAAGAATCGTACTCTTACATATGACAAGCTTATTATTGC
AACAGGTTCTAGTGCTTTTATTTTACCTGTTGAAGGTTCCACTCTTCCTGGTGTAACAGGTTTTCGAACCATTGAAGATA
CACAGTTTATGATGGATACTGCTAAAGAAAAGAAAAAGGCCGTTGTCATTGGTGGTGGATTACTTGGTTTAGAAGCAGCA
CGAGGTCTTATTGACTTAGGAATGGACGTACATGTTGTTCATTTAATGCCTAGCTTAATGGAGCAACAACTAGATACGAA
AGCCGCTTCTCTACTGCGTGAAGATTTAGAAGCACAAGGCATGAAGTTTCTCATGGAAAAGAAAACTGTAAAAATTCTTG
GTACAAACCATGTTGAGGGCATTCAATTTGAAGATGGTGAAGTTGTAGATTGTGATTTAATCGTAATGGCTGTCGGAATC
CGACCAAATACGCAAATAGCAAGAGATGCTGGTTTAATTGTCAATCGCGGCATTGTAGTCAATGACTATATGCTAACAAA
TGATGAGTCCATTTATGCAGTTGGCGAATGTGCAGAACATGACGGTATCGCATATGGACTTGTTGCCCCTCTTTATGAAC
AAGGTGCAATATTAGCGAAACATATATCGAATTTACAAACTGATGGATATTCGGGCAGTATCGTCGGTACGCAATTAAAA
GTTGCTGGTTGTGATTTATTCTCTGCAGGCCAAATTTATGAAGATGATCAAACGAAAGCAATATCCATCTTTAATGAATG
CAAACGTTCCTATAAAAAAGTATTAATTCGTGACAATAAAGTCGTCGGTATCGTTTTATATGGTGACACAGCTGATGGTA
CACGCCTCTTTAGCATGTTAAAGAAAGAAGAAGATATACAAGAATATACACCAGCGTCCATTCTTCACAAAGCTGGTGAA
GAAAGTGAACTTGATGTTGCTACAATGAGTGCGGATGACACGATTTGTGGATGTAATGGTGTTACGAAAGGAACAATCGT
TCATGCCATTTTAGAACAAGAGTTAACGACTTTTGAAGAAGTTAAAGGCTGTACGAAAGCCGCAGGGTCTTGTGGTAAAT
GTCGTCCGCTTGTAGAACAAGTTTTATCTCATACACTCGGAGATGCTTTTGATGCCTCAGCCCAGTCTGCTGGTATGTGC
GGATGTACACCTTTATCACGTGATGAAGTCGTAGCGGCAATTCATGAAAAAGGCTTAAAATCACCAAAAGAAGTACGAAA
TGTTCTTGGTTTTGTACATGAAGATGGCTGTTCAAAATGTCGTCCTGCTTTGAACTACTATTTACGTATGGCGATTCCAG
AAGAATATGAAGATGATAAAGCCTCCCGTTTCGTTAATGAAAGAATGAATGGTAACATCCAGCACGATGGTACATTCTCT
GTTATTCCACGTATGTACGGAGGCGTTACAACAGCAGATGATTTAATGAAAATTGCTGAAGTTGCGAAAAAGTATGATGT
TCCACTTGTGAAAATTACCGGTGCAAGTCGAATTGGCTTATACGGTGTTAAGAAACAAGATTTACCTAACGTATGGGCTG
ACTTAAATATGACTTCTGGATATGCGTATTCAAAATCTCTTCGTAATGTAAAATCATGTGTTGGCTCTCGTTTCTGCCGT
TTCGGTACGAAAGATTCATTAGGACTTGGTATGCTCCTTGAACAATCATTAGAAATGGTAGATACACCTCATAAAATGAA
GATGGGTGTAACGGGCTGTCCGCGTAACTGTGCGGAAGTACTTACGAAAGATTTTGGCGTTGTTTGTGTCGAAAATGGAT
ACCAACTTTATATTGGTGGAAATGGTGGTACAGAAGTACGTGAAGCTGATTTTGTAATGATTGTCCCTACTGAAGATGAT
GTTCTTCACATCGCTACAGCTTACATGCAATATTATCGTGAAACTGGTATTTACGGAGAGCGTACTGCCTACTGGACAGA
ACGATTAGACTTCGATCACATAAAAGAAATACTGCAAGATGCAAATATGGTCACTAAGTTAAATGAACGTTTCCAAACGG
CCCGTGGCACATATAAAGAAGCTTGGGGACAAGCACTAGAAACGAAATCATTAAAAGCGATGTATGAAGTAGAAACTGTG
AAATAA

Upstream 100 bases:

>100_bases
TCAAAAGTGTGATAAATATCACATCTCCCCCTTTTTTGATTTTCTATAATAGAAATATAATCCTTTGCTCAATACTTCAC
ATAAAAAAGGGGAAACGAAA

Downstream 100 bases:

>100_bases
GGAGCTGTGATCTATATGATACAAACGAAAGATAAAATAAAAGTTATGCGTGCAGAAGATCTTCCTATTCAAATCGGTAA
AGAGGTCCAAATGAAAGGTA

Product: nitrite reductase [NAD(P)H], large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIMTEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLDFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Sequences:

>Translated_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIMTEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLDFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K
>Mature_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIMTEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLDFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=27.1739130434783, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=27.1739130434783, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=27.1739130434783, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI22202629, Length=266, Percent_Identity=26.6917293233083, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI4757732, Length=266, Percent_Identity=26.6917293233083, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1789765, Length=816, Percent_Identity=35.0490196078431, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=29.7734627831715, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=24.6428571428571, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=405, Percent_Identity=22.962962962963, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32565766, Length=210, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281359715, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI281359713, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24639250, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI18543267, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24639252, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24639257, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585130, Length=347, Percent_Identity=22.478386167147, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88727; Mature: 88727

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIMTEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCCEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCHHHHH
HISNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEESELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLDFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIMTEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCCEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCHHHHH
HISNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEESELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLDFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]