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The map label for this gene is dat1 [H]

Identifier: 227814929

GI number: 227814929

Start: 2134512

End: 2135387

Strand: Reverse

Name: dat1 [H]

Synonym: BAMEG_2340

Alternate gene names: 227814929

Gene position: 2135387-2134512 (Counterclockwise)

Preceding gene: 227814930

Following gene: 227814928

Centisome position: 40.83

GC content: 34.02

Gene sequence:

>876_bases
ATGGAAGCTACTCATAAAGATTGGATTTTATTTAACGGAAGAATTGTAAATACGAAGGAAGAACAACCGATGATTCCATT
AGAGGAGAGAGGCTTTCAATTTGGTGATGGTATATACGAGGTATTCAGACTATATGATGGGAAGCCACATTTATTAGATT
TACATTTAGAACGATTCTTTCATTCTATGGAAGAAATAAAATTAATTCCACCATTTACTAAGGAAGAGTTGGCGGAAGAG
TTACATCAAATGATTGAAAAAAATCAATTTCAAGAAGATGGGAATGTATATTTGCAAATATCAAGAGGCGCCCAAGCACG
TAATCATGTATATGAGTCAAATATGCAACCAACATATTTTGCAAATATTGTTTCGTTTCCACGACCAATTGCTACTATGG
AACAAGGAATAAAAGTTACTGTAGAAGAGGATATACGCTGGAAGTTTTGTCATATAAAATCTTTAAATCTGCTACCTAAT
ATTATGATTAAAAATAAAATAAACGAACAAGGCTATCAAGAAGCGATATTAGTGCGAGATGGAATTGTAACGGAAGGTTG
TCATTCAAATTTCTTTATGGTGAAAAATAATAAATTGATTACACATCCGGCTGACAATTTCATTCTACACGGCATTACAC
GTCATTATGTTATTACATTAGCGAAGGAGTTACATATTGAAGTAGAAGAAAGAGAATTTTCACTTCAAGAAGTATATGAG
GTCGATGAGTGCTTCTTCACAGCGACACCACTTGAAATATTCCCAGTCGTTCAAATTGGTGACGAGCAGTTTGGAAACGG
AGAAAGAGGGCCAATTACAAAGAGACTCCAAGTTGCATATGAAGAAAGTATTCGTTTGTTTAAAGTTACGAACTAA

Upstream 100 bases:

>100_bases
TGATGCATTTTTTATTTTGTTAAAATACAGAAAATTCCTTCTTTTGAGTGTGGATAAGTTGTACAATTAAAATAATCATG
GAATAAAGGAGTGGGAATAA

Downstream 100 bases:

>100_bases
TACTATGAAAAAAAGCTGGTATTCCTCCAGCTTTTTTTCATGATATAATTCAAGCATAGGACAAGATTTGAAAACGCTGT
AAAAAGGGGATTTAAGGTAT

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MEATHKDWILFNGRIVNTKEEQPMIPLEERGFQFGDGIYEVFRLYDGKPHLLDLHLERFFHSMEEIKLIPPFTKEELAEE
LHQMIEKNQFQEDGNVYLQISRGAQARNHVYESNMQPTYFANIVSFPRPIATMEQGIKVTVEEDIRWKFCHIKSLNLLPN
IMIKNKINEQGYQEAILVRDGIVTEGCHSNFFMVKNNKLITHPADNFILHGITRHYVITLAKELHIEVEEREFSLQEVYE
VDECFFTATPLEIFPVVQIGDEQFGNGERGPITKRLQVAYEESIRLFKVTN

Sequences:

>Translated_291_residues
MEATHKDWILFNGRIVNTKEEQPMIPLEERGFQFGDGIYEVFRLYDGKPHLLDLHLERFFHSMEEIKLIPPFTKEELAEE
LHQMIEKNQFQEDGNVYLQISRGAQARNHVYESNMQPTYFANIVSFPRPIATMEQGIKVTVEEDIRWKFCHIKSLNLLPN
IMIKNKINEQGYQEAILVRDGIVTEGCHSNFFMVKNNKLITHPADNFILHGITRHYVITLAKELHIEVEEREFSLQEVYE
VDECFFTATPLEIFPVVQIGDEQFGNGERGPITKRLQVAYEESIRLFKVTN
>Mature_291_residues
MEATHKDWILFNGRIVNTKEEQPMIPLEERGFQFGDGIYEVFRLYDGKPHLLDLHLERFFHSMEEIKLIPPFTKEELAEE
LHQMIEKNQFQEDGNVYLQISRGAQARNHVYESNMQPTYFANIVSFPRPIATMEQGIKVTVEEDIRWKFCHIKSLNLLPN
IMIKNKINEQGYQEAILVRDGIVTEGCHSNFFMVKNNKLITHPADNFILHGITRHYVITLAKELHIEVEEREFSLQEVYE
VDECFFTATPLEIFPVVQIGDEQFGNGERGPITKRLQVAYEESIRLFKVTN

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=302, Percent_Identity=29.1390728476821, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787338, Length=253, Percent_Identity=26.0869565217391, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 34088; Mature: 34088

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATHKDWILFNGRIVNTKEEQPMIPLEERGFQFGDGIYEVFRLYDGKPHLLDLHLERFF
CCCCCCCEEEECCEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHH
HSMEEIKLIPPFTKEELAEELHQMIEKNQFQEDGNVYLQISRGAQARNHVYESNMQPTYF
HHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCHHH
ANIVSFPRPIATMEQGIKVTVEEDIRWKFCHIKSLNLLPNIMIKNKINEQGYQEAILVRD
HHHHHCCCCHHHHHCCCEEEEECCCEEEEEEEEECCCCCCCCEECCCCCCCHHEEEEEEC
GIVTEGCHSNFFMVKNNKLITHPADNFILHGITRHYVITLAKELHIEVEEREFSLQEVYE
CCEECCCCCCEEEEECCEEEECCCCCCEEHHHHHHHHHHEEHHHEEEEHHHHCCHHHHHH
VDECFFTATPLEIFPVVQIGDEQFGNGERGPITKRLQVAYEESIRLFKVTN
HHHHEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MEATHKDWILFNGRIVNTKEEQPMIPLEERGFQFGDGIYEVFRLYDGKPHLLDLHLERFF
CCCCCCCEEEECCEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHH
HSMEEIKLIPPFTKEELAEELHQMIEKNQFQEDGNVYLQISRGAQARNHVYESNMQPTYF
HHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCHHH
ANIVSFPRPIATMEQGIKVTVEEDIRWKFCHIKSLNLLPNIMIKNKINEQGYQEAILVRD
HHHHHCCCCHHHHHCCCEEEEECCCEEEEEEEEECCCCCCCCEECCCCCCCHHEEEEEEC
GIVTEGCHSNFFMVKNNKLITHPADNFILHGITRHYVITLAKELHIEVEEREFSLQEVYE
CCEECCCCCCEEEEECCEEEECCCCCCEEHHHHHHHHHHEEHHHEEEEHHHHCCHHHHHH
VDECFFTATPLEIFPVVQIGDEQFGNGERGPITKRLQVAYEESIRLFKVTN
HHHHEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]