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The map label for this gene is kinB2 [H]

Identifier: 227814539

GI number: 227814539

Start: 1774701

End: 1775969

Strand: Direct

Name: kinB2 [H]

Synonym: BAMEG_1947

Alternate gene names: 227814539

Gene position: 1774701-1775969 (Clockwise)

Preceding gene: 227814538

Following gene: 227814544

Centisome position: 33.93

GC content: 28.61

Gene sequence:

>1269_bases
ATGAACTTTGTGTATATCAATCAACACGTGTTAAATAACCTTTTATACATTTTAAGTAGTATATTTATTTTTTATTTTAT
ATATGATAGCGGGCGCTATTTAAAGAAATATAAAAAACTACTTATTATACTTTGTACGAGTATCCCTCTTATTTTATGTA
TGCGTTATCCTATCTATATGGATGAAAATTGTATTCATGATTTAAGACAAATACCCGTTATAATAGGTACGCTTTATGGT
GGCTTTCCTGTCGGTATAACATTGTTTGCTATTTTATTAATTACAAGATTTTCATTTTATGGATTTAGTATGTTAACAAT
AGTAGTGTATGGCACAATGTTTATCATTACAGCATTCGCATCCGCAAAATTTAATACGTATAATAGAAAAATCAAAGTAA
CTTATGCAATGTTTTTAACATTTTTTCTGGCAATATTTACAACTGTAATTGTATTAACTTTATCAGATTTTGAAGTGAAT
AATTTGTATATTATTTATTTCATAATATTACCAACTATTTTAATGTTGTTTGTCGTTTATTTTAATGAAGTTTTAAAAGA
TGCATTATGTATGCGATCGAAGTTAATTAAAATGGAAAAAATGGAGATTGTTAGCCAACTCGCTGCTAGTATATCGCACG
AAGTAAGAAATCCCTTAACTGTCGTAAAAGGATTTACTCAACTTTTAAAAACACCAAATTTAACTCCAGAATCAAGAGAT
GAGTATATTAAGCATATACTAGAAGAATTACATCGTGCTCAAGGAATTATTGATGATTATTTAACGTTTGCCAAACCTGC
TTCAGAAAAATTAGATGAGATTTCAGTAGAACAAGAGTTAAATCGAGTTATTAATATGATCTTACCTTTATGTAATATGA
ATACAATACATATTACAAAAGATTTCTCTATGGCAACAATAGTTGGTAATAAACAGCACTTTCAGCAATGTTTTTTAAAT
TTAATAAAAAATGGTATTGAAGCAATGCCTAATGGCGGTACTTTAAATATCTCCTCATCTATTAGTAATAATAAGGTTAT
CATACGTATTGAAGATAGCGGAATTGGTATGTCACAAGAGCAAGTAAATCGATTTGGTGAACCGTATTTTAATACAAAAA
CGAAAGGTACAGGCTTAGGTACAATGGTTGCTGTTAAGATCATTGAAACGATGCAAGGTAGTTTAAGAATCCGAAGCGTC
GTTAATAAAGGTACCACTTTAACAATTACATTTCCGAAATGTAGTCAGAACTCATCTGATAACAAATAA

Upstream 100 bases:

>100_bases
TAAAAAGATATAAACCTATATTATTCAAACAAATATATAGTTTATACTATTATCATGATAATATATATGAAAAGGAAATA
AAAATAACGAGGCAGATATT

Downstream 100 bases:

>100_bases
AAAAGGAGCAATTATGCTCCTTTTTTATTTGTTTCGATACATAAGTATCTTCTATGTTTTTTTAGTTTACATTTGTGGAG
ACTAATTAGAAATTAGTACC

Product: sporulation kinase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNFVYINQHVLNNLLYILSSIFIFYFIYDSGRYLKKYKKLLIILCTSIPLILCMRYPIYMDENCIHDLRQIPVIIGTLYG
GFPVGITLFAILLITRFSFYGFSMLTIVVYGTMFIITAFASAKFNTYNRKIKVTYAMFLTFFLAIFTTVIVLTLSDFEVN
NLYIIYFIILPTILMLFVVYFNEVLKDALCMRSKLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTPNLTPESRD
EYIKHILEELHRAQGIIDDYLTFAKPASEKLDEISVEQELNRVINMILPLCNMNTIHITKDFSMATIVGNKQHFQQCFLN
LIKNGIEAMPNGGTLNISSSISNNKVIIRIEDSGIGMSQEQVNRFGEPYFNTKTKGTGLGTMVAVKIIETMQGSLRIRSV
VNKGTTLTITFPKCSQNSSDNK

Sequences:

>Translated_422_residues
MNFVYINQHVLNNLLYILSSIFIFYFIYDSGRYLKKYKKLLIILCTSIPLILCMRYPIYMDENCIHDLRQIPVIIGTLYG
GFPVGITLFAILLITRFSFYGFSMLTIVVYGTMFIITAFASAKFNTYNRKIKVTYAMFLTFFLAIFTTVIVLTLSDFEVN
NLYIIYFIILPTILMLFVVYFNEVLKDALCMRSKLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTPNLTPESRD
EYIKHILEELHRAQGIIDDYLTFAKPASEKLDEISVEQELNRVINMILPLCNMNTIHITKDFSMATIVGNKQHFQQCFLN
LIKNGIEAMPNGGTLNISSSISNNKVIIRIEDSGIGMSQEQVNRFGEPYFNTKTKGTGLGTMVAVKIIETMQGSLRIRSV
VNKGTTLTITFPKCSQNSSDNK
>Mature_422_residues
MNFVYINQHVLNNLLYILSSIFIFYFIYDSGRYLKKYKKLLIILCTSIPLILCMRYPIYMDENCIHDLRQIPVIIGTLYG
GFPVGITLFAILLITRFSFYGFSMLTIVVYGTMFIITAFASAKFNTYNRKIKVTYAMFLTFFLAIFTTVIVLTLSDFEVN
NLYIIYFIILPTILMLFVVYFNEVLKDALCMRSKLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTPNLTPESRD
EYIKHILEELHRAQGIIDDYLTFAKPASEKLDEISVEQELNRVINMILPLCNMNTIHITKDFSMATIVGNKQHFQQCFLN
LIKNGIEAMPNGGTLNISSSISNNKVIIRIEDSGIGMSQEQVNRFGEPYFNTKTKGTGLGTMVAVKIIETMQGSLRIRSV
VNKGTTLTITFPKCSQNSSDNK

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=278, Percent_Identity=26.2589928057554, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=27.6595744680851, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789149, Length=232, Percent_Identity=29.7413793103448, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=28.0487804878049, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=28.6343612334802, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786600, Length=242, Percent_Identity=23.1404958677686, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=226, Percent_Identity=24.3362831858407, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790861, Length=232, Percent_Identity=21.9827586206897, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48147; Mature: 48147

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVYINQHVLNNLLYILSSIFIFYFIYDSGRYLKKYKKLLIILCTSIPLILCMRYPIYM
CCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DENCIHDLRQIPVIIGTLYGGFPVGITLFAILLITRFSFYGFSMLTIVVYGTMFIITAFA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAKFNTYNRKIKVTYAMFLTFFLAIFTTVIVLTLSDFEVNNLYIIYFIILPTILMLFVVY
HCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHH
FNEVLKDALCMRSKLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTPNLTPESRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHH
EYIKHILEELHRAQGIIDDYLTFAKPASEKLDEISVEQELNRVINMILPLCNMNTIHITK
HHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
DFSMATIVGNKQHFQQCFLNLIKNGIEAMPNGGTLNISSSISNNKVIIRIEDSGIGMSQE
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCHH
QVNRFGEPYFNTKTKGTGLGTMVAVKIIETMQGSLRIRSVVNKGTTLTITFPKCSQNSSD
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
NK
CC
>Mature Secondary Structure
MNFVYINQHVLNNLLYILSSIFIFYFIYDSGRYLKKYKKLLIILCTSIPLILCMRYPIYM
CCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DENCIHDLRQIPVIIGTLYGGFPVGITLFAILLITRFSFYGFSMLTIVVYGTMFIITAFA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAKFNTYNRKIKVTYAMFLTFFLAIFTTVIVLTLSDFEVNNLYIIYFIILPTILMLFVVY
HCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHH
FNEVLKDALCMRSKLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTPNLTPESRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHH
EYIKHILEELHRAQGIIDDYLTFAKPASEKLDEISVEQELNRVINMILPLCNMNTIHITK
HHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
DFSMATIVGNKQHFQQCFLNLIKNGIEAMPNGGTLNISSSISNNKVIIRIEDSGIGMSQE
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCHH
QVNRFGEPYFNTKTKGTGLGTMVAVKIIETMQGSLRIRSVVNKGTTLTITFPKCSQNSSD
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]