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The map label for this gene is folK-2 [H]

Identifier: 227080776

GI number: 227080776

Start: 577819

End: 578322

Strand: Reverse

Name: folK-2 [H]

Synonym: VCM66_0551

Alternate gene names: 227080776

Gene position: 578322-577819 (Counterclockwise)

Preceding gene: 227080777

Following gene: 227080775

Centisome position: 19.99

GC content: 51.98

Gene sequence:

>504_bases
ATGATGCTTGCCTACATTGCGATTGGCAGTAATTTGGGCGACCCTGTCGCCCAAGCACAGCTGGCCATACAACAGCTTAC
TCTCTTACCGAAGTCGCGCCTTATCGCGGCTTCATCGCTCTATAGCAGCACTCCGATGGGGCCGCAAAATCAGCCTGATT
ACATCAACGCTGTCGCCGCCATCGAAACCGAATTAACGCCACTGGAATTGCTCGATTGCACACAACGCATTGAGCTAGAA
CAGGGGCGTGTCCGTAAAAGCGAACGATGGGGACCGAGAACCCTCGATCTCGATATTGTGCTTTACGGCAATGAGGTGAT
AGAGACCGAGCGCCTCACCATTCCTCACTATGGAATGAAAGTACGTGAATTCGTGCTTTACCCGCTCGCGGAAATCGCAC
CAAATTTAACCCTCCCAGACGGGACTGAAATCCAAGCGTTATTGCAACAAGTACCGCTCAATGGGCTCAGCATTTGGCAC
TCGCCAACGTCGAGTAAGGACTAA

Upstream 100 bases:

>100_bases
AAGCCATGGTGGGTGATATCGGTAATGCAACGGGCAGCAGCAAATCTGGCGCTCGCCGCCGTAAACCATCCCGCAACAAA
TCCAAGAGTAAATCCAGCGA

Downstream 100 bases:

>100_bases
ACATGAAAAAAATTACCATAAACGACCTGATGAAATGGAAGCAGGAAGGTCGCAAATTTGCTACATCAACCGCTTATGAT
GCGAGTTTTGCTCAGTTATT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MMLAYIAIGSNLGDPVAQAQLAIQQLTLLPKSRLIAASSLYSSTPMGPQNQPDYINAVAAIETELTPLELLDCTQRIELE
QGRVRKSERWGPRTLDLDIVLYGNEVIETERLTIPHYGMKVREFVLYPLAEIAPNLTLPDGTEIQALLQQVPLNGLSIWH
SPTSSKD

Sequences:

>Translated_167_residues
MMLAYIAIGSNLGDPVAQAQLAIQQLTLLPKSRLIAASSLYSSTPMGPQNQPDYINAVAAIETELTPLELLDCTQRIELE
QGRVRKSERWGPRTLDLDIVLYGNEVIETERLTIPHYGMKVREFVLYPLAEIAPNLTLPDGTEIQALLQQVPLNGLSIWH
SPTSSKD
>Mature_167_residues
MMLAYIAIGSNLGDPVAQAQLAIQQLTLLPKSRLIAASSLYSSTPMGPQNQPDYINAVAAIETELTPLELLDCTQRIELE
QGRVRKSERWGPRTLDLDIVLYGNEVIETERLTIPHYGMKVREFVLYPLAEIAPNLTLPDGTEIQALLQQVPLNGLSIWH
SPTSSKD

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=58.4905660377358, Blast_Score=200, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324073, Length=134, Percent_Identity=41.044776119403, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18494; Mature: 18494

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLAYIAIGSNLGDPVAQAQLAIQQLTLLPKSRLIAASSLYSSTPMGPQNQPDYINAVAA
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
IETELTPLELLDCTQRIELEQGRVRKSERWGPRTLDLDIVLYGNEVIETERLTIPHYGMK
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEEEECCCEECCCCCHH
VREFVLYPLAEIAPNLTLPDGTEIQALLQQVPLNGLSIWHSPTSSKD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MMLAYIAIGSNLGDPVAQAQLAIQQLTLLPKSRLIAASSLYSSTPMGPQNQPDYINAVAA
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
IETELTPLELLDCTQRIELEQGRVRKSERWGPRTLDLDIVLYGNEVIETERLTIPHYGMK
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEEEECCCEECCCCCHH
VREFVLYPLAEIAPNLTLPDGTEIQALLQQVPLNGLSIWHSPTSSKD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]