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The map label for this gene is dnaG [H]

Identifier: 227080701

GI number: 227080701

Start: 509828

End: 511591

Strand: Reverse

Name: dnaG [H]

Synonym: VCM66_0476

Alternate gene names: 227080701

Gene position: 511591-509828 (Counterclockwise)

Preceding gene: 227080702

Following gene: 227080700

Centisome position: 17.69

GC content: 46.43

Gene sequence:

>1764_bases
ATGGCAGGACACATCCCCCGCAGTTTTATTGATGACCTCCTTGCTCGACTCGATATTGTCGACGTTATTGACGCACGCGT
AAAACTGAAGAAGAAAGGCAAAAACTACAGCGCCTGCTGCCCTTTCCATAACGAAAAAACCCCTTCATTCAGTGTCAGTC
AGGAAAAACAGTTCTATCACTGCTTCGGCTGTGGTGCTCATGGCAATGCCATCGATTTTGTCATGGAATATGAACGCATG
GAGTTTCCTGAGGCCATTGAAGAGCTGGCGTCGACCATAGGTCTTGAAGTTCAGCGTGAAGAAAGAACGCCTTCCAGCCC
CTTTGCGAAAAATACGCCAGCCGTCAAAAGTGAAGATAAGCGCAGCCTGTATGACTTGATGGGCAGCATCGCCCAGTTTT
ATCGTCAGCAGCTTAAAGTTCCTGCCAACAAGCATGCGATTGAATACTTAAAAAATCGTGGTTTATCTGGAGAAATCGTC
CAGAAATTTGGCATTGGATACATAGCGGATGAATGGGATCTTGTACGGCGCAATTTTGGTCAGCAGCGCAGCAGTGAAGA
TATGCTTGTCACCGCAGGTATGCTGATTGAAAACGATAACGGCCGCCGTTATGACCGCTTTCGCGGACGAGTCATGTTCC
CAATTCGCGATCGTCGAGGTCGAGTCATTGGTTTTGGTGGCCGTATTACAGAGCAAGGTACACCGAAATACCTGAACTCG
CCTGAAACCCCGATTTTCCATAAAGGCAAAGAGCTGTATGGCCTTTATGAAGTCCTGCAGGCATACCGTGAACCACCACA
AATCCTAGTGGTGGAAGGCTATATGGATGTCGTGGCACTCGCTCAATATGGTGTCGATTACTCTGTAGCCTCTCTGGGCA
CCTCAACCACAGGCGATCACTTACAACTGTTGTTTCGCCAAACTAACCATGTCATCTGTTGTTATGATGGTGACCGAGCT
GGACGTGATGCCGCATGGCGCGCATTAGAAAATGCACTCAGCTACCTCAAAAGCGGCAATATCTTAAAGTTTCTGTTTCT
GCCGGACGGTGAAGATCCCGATAGCTACGTGCGTAAATACGGCAAAGCCGATTTTGAACAACAGGTTGCCAATGCTACAC
CACTGTCGCAGTACTTATTTGAGAACTTGATTGAACTGCACCAAATTAACTTGGGTAGCCATGAAGGTAAATCGGCACTG
CGCGCCGTTGCAACACCACTTATCGACAAAATTCCGGATCCTTTTTTACAAGAGATCCTAGAGAAATTACTCGATGAACG
GACAGGCTTTGATCACCAACTGCGCCGTAAAAAAGCACGTAAGACAGAAAATCGACCCGCACCACATAAAGCAATAAAAC
GGACCCCAATGCGTGATGTTATCGCGCTCTTGATACAAAATCCGAGCTATGCTGAATTGGTACCAGACCTAGCGAGCGTG
CGTCATTTGATGATCCCTGGTCTCGATACCTTCAGTGAAGTACTTGAGAAATGTCGTCAATATCCCCATATCACTACGGG
TCAATTACTCGAACATTGGCGAGACTCAAAAAATGAGACACTTCTGTCTCGTCTGGCAAGTTGGGAAATTCCCCTTGTCG
AAGACAATCAAGAAGAACTATTTTTAGACTCATTGGACAAAATTCTTGCCCAGTGCGTAGAAAAACAAATTGAAAATTTG
CAGGCCAAAGAGCGAAGTGTCGGTTTATCAACCGAAGAGAAAAGAGAGCTACAAGACTTGATCTTAAAAGGCTTGAAAGC
GTAA

Upstream 100 bases:

>100_bases
AACTGGCTTAATCCCAACCCAATTACAGCAAGCCGTGCAATCCTTTGGAAAGCACGGCTTGTTTGTATTTGACCCTATCT
TTCGGTGCATGCAAAGATTT

Downstream 100 bases:

>100_bases
CTCTGTTGAGATAGTCAGCAAAGAATAAATTTGTTAGTATACGTGGTTTGCATTGCGCATACTTATTCCTTCACCAGATA
CGAAGTTGGATACCGTCTAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYHCFGCGAHGNAIDFVMEYERM
EFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIV
QKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNS
PETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHLQLLFRQTNHVICCYDGDRA
GRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSAL
RAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMRDVIALLIQNPSYAELVPDLASV
RHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQEELFLDSLDKILAQCVEKQIENL
QAKERSVGLSTEEKRELQDLILKGLKA

Sequences:

>Translated_587_residues
MAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYHCFGCGAHGNAIDFVMEYERM
EFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIV
QKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNS
PETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHLQLLFRQTNHVICCYDGDRA
GRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSAL
RAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMRDVIALLIQNPSYAELVPDLASV
RHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQEELFLDSLDKILAQCVEKQIENL
QAKERSVGLSTEEKRELQDLILKGLKA
>Mature_586_residues
AGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYHCFGCGAHGNAIDFVMEYERME
FPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIVQ
KFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNSP
ETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHLQLLFRQTNHVICCYDGDRAG
RDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSALR
AVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMRDVIALLIQNPSYAELVPDLASVR
HLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQEELFLDSLDKILAQCVEKQIENLQ
AKERSVGLSTEEKRELQDLILKGLKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=587, Percent_Identity=54.5144804088586, Blast_Score=633, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66939; Mature: 66808

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCEEE
CFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDK
EECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
RSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHC
QQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNS
CCCCCCCEEEEECEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCCCCC
PETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDH
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHHHCCCCCCCHH
LQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKY
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
GKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSALRAVATPLIDKIPDPFLQEIL
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
EKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMRDVIALLIQNPSYAELVPDLASV
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHH
RHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQEEL
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCHHHH
FLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQDLILKGLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCEEE
CFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDK
EECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
RSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHC
QQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNS
CCCCCCCEEEEECEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCCCCC
PETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDH
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHHHCCCCCCCHH
LQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKY
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
GKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSALRAVATPLIDKIPDPFLQEIL
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
EKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMRDVIALLIQNPSYAELVPDLASV
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHH
RHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQEEL
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCHHHH
FLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQDLILKGLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]